Tributyl phosphate

Repeated dose toxicity: oral

Administrative data

Endpoint:

sub-chronic toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

1 (reliable without restriction)

Rationale for reliability incl. deficiencies:

other: guideline study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1991

Report date:

1991

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

EPA OTS 798.2650 (90-Day Oral Toxicity in Rodents)

Principles of method if other than guideline:

Tributyl phosphate was administered in the diet to CD-1 mice (15/sex/group at study initiation) at concentrations of 0, 500, 2000, or 8000 ppm for ninety days. Control animals received standard laboratory diet.

GLP compliance:

yes

Test material

Test material information

Constituent 1

Reference substance name:

Tributyl phosphate

EC Number:

204-800-2

EC Name:

Tributyl phosphate

Cas Number:

126-73-8

Molecular formula:

C12H27O4P

IUPAC Name:

tributyl phosphate

Details on test material:

purity 99.7 %

Test animals

Species:

mouse

Strain:

CD-1

Sex:

male/female

Administration / exposure

Route of administration:

oral: feed

Vehicle:

other: diet

Analytical verification of doses or concentrations:

yes

Duration of treatment / exposure:

3 month

Frequency of treatment:

daily (feeding study)

Doses / concentrations

Remarks:

Doses / Concentrations:
500, 2000 and 8000 mg/kg diet (75, 300 and 1200 mg/kg bw/day)
Basis:

No. of animals per sex per dose:

15/sex/group

Control animals:

yes

Details on study design:

Post-exposure period: no

Results and discussion

Effect levels

Dose descriptor:

NOEL

Effect level:

500 ppm

Sex:

male/female

Basis for effect level:

other: 500 ppm = 75 mg/kg bw

Target system / organ toxicity

Critical effects observed:

not specified

Any other information on results incl. tables

All animals survived, in the highest concentration body weight loss and reduced body weight gain with reduced food
consumption and reduced fecal volume, elevation of absolute
and relative liver weights with hepatocyte hypertrophy,
slight to moderate epithelial hyperplasia of the urinary
bladder, some slight hematological alterations and some
effects on clinical chemistry parameters of liver function.
In the middle concentration slight decrease of weight gains,
elevated terminal ALT and AST in females and moderately
elevated liver weights in both sexes, slight hepatocyte
hypertrophy and minimal or slight epithelial hyperplasia of
the urinary bladder.

No effects of test material administration was evident in

animals receiving the lowest concentration (500 ppm).
NOEL: 75 mg/kg bw/day

Applicant's summary and conclusion

Executive summary:

Tributyl phosphate was administered in the diet to CD-1 mice (15/sex/group at study initiation) at concentrations of 0, 500, 2000, or 8000 ppm for ninety days. Control animals received standard laboratory diet. All animals survived until their sheduled termination dates. No effects of test material administration were evident in animals receiving the lowest concentration (500 ppm = 75 mg/kg bw).