Registration Dossier
Registration Dossier
Diss Factsheets
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EC number: 204-800-2 | CAS number: 126-73-8
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Proprietary GLP study similar to guidelines, summarised in OECD SIDS (secondary source). Officially accepted and peer-reviewed by the OECD SIDS evaluation programme.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant - Analytical monitoring:
- no
- Details on sampling:
- No information available
- Vehicle:
- not specified
- Details on test solutions:
- Static freshwater
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Pimephales promelas, fathead minnow
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information available
- Hardness:
- No information available
- Test temperature:
- No information available
- pH:
- No information available
- Dissolved oxygen:
- No information available
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Nominal
- Details on test conditions:
- No further information available
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 6.4 mg/L
- Nominal / measured:
- nominal
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 3.2 mg/L
- Nominal / measured:
- nominal
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 10 mg/L
- Nominal / measured:
- nominal
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 9.6 mg/L
- Nominal / measured:
- nominal
- Details on results:
- The 96 hour LC50 of tributyl phosphate to fathead minnows was 6.4mg/l
- Results with reference substance (positive control):
- Not relevant
- Reported statistics and error estimates:
- No information available
- Sublethal observations / clinical signs:
The 96-hour LC50 of tributyl phosphate to fathead minnows was 6.4 mg/L.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96 hour LC50 of tributyl phosphate to fathead minnows was 6.4mg/l, the 96 hour NOEC was 3.2mg/l.
- Executive summary:
The 96-hour LC50 of tributyl phosphate to fathead minnows was 6.4 mg/L, the 96 hour NOEC was 3.2mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: English abstract of Japanese paper
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Acute toxicity in Oryzias latipes
- GLP compliance:
- no
- Remarks:
- : literature study
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
not applicable - Analytical monitoring:
- not specified
- Details on sampling:
- No information provided
- Vehicle:
- yes
- Details on test solutions:
- No information provided
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- No information provided
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No information provided
- Hardness:
- No information provided
- Test temperature:
- No information provided
- pH:
- No information provided
- Dissolved oxygen:
- No information provided
- Salinity:
- No information provided
- Nominal and measured concentrations:
- No information provided
- Details on test conditions:
- Tributyl phosphate was subjected to the LC50 test by using small static systems at low (10 °C), medium (20 °C) and high (30 °C) temperatures. Permissable volume of various dissolvents was determined at 30 °C A 10 % ether-dimethylsulfoxide solution was chosen as a suitable dissolvent for water insoluble pollutants.
- Reference substance (positive control):
- not specified
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 18.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 10 degrees Celsius
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 18.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 10 degrees Celsius
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 18.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 20 degrees Celsius
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 18.5 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 20 degrees Celsius
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5.85 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 30 degrees Celsius
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 5.85 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: 30 degrees Celsius
- Details on results:
- No further information provided
- Results with reference substance (positive control):
- Not relevant
- Reported statistics and error estimates:
- No information provided
- Sublethal observations / clinical signs:
24 -hour and 48 -hour LC50 values of 18.5 mg/L were calculated for exposure at 10 and 20 degrees Celsius. Greater toxicity was seen at 30 degrees Celsius, with LC50 values of 5.85 mg/L at 24 and 48 hours.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- 24 -hour and 48 -hour LC50 values of 18.5 mg/L were calculated for exposure at 10 and 20 degrees Celsius. Greater toxicity was seen at 30 degrees Celsius, with LC50 values of 5.85 mg/L at 24 and 48 hours.
- Executive summary:
The influence of temperature on the toxicity of chemicals to Oryzias latipes was investigated. Groups of fish were exposed to the test material at 10, 20 or 30 degrees Celsius for up to 48 hours.
24 -hour and 48 -hour LC50 values of 18.5 mg/L were calculated for exposure at 10 and 20 degrees Celsius. Greater toxicity was seen at 30 degrees Celsius, with LC50 values of 5.85 mg/L at 24 and 48 hours. The acute toxicity of tributyl phosphate to Oryzias latipes increases with increased temperature.- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Proprietary guideline study, summarised in OECD SIDS (secondary source). Officially accepted and peer-reviewed by the OECD SIDS evaluation programme.
- Qualifier:
- according to guideline
- Guideline:
- other: "Methods for Acute Toxicity Tests with Fish, Macroinvertebrates and Amphibians" EPA Ecological Research Series EPA-660/3-75-009, April 1975, US EPA-TSCA, 40 CFR, Part 797 (1985)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant - Analytical monitoring:
- not specified
- Details on sampling:
- No information available
- Vehicle:
- not specified
- Details on test solutions:
- No information available
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Fresh water fish, Oncorhynchus mykiss, rainbow trout.
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information available
- Hardness:
- No information available
- Test temperature:
- 12 °C
- pH:
- No information available
- Dissolved oxygen:
- No information available
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- No information available
- Details on test conditions:
- No information available
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 4.3 mg/L
- Nominal / measured:
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 19 mg/L
- Nominal / measured:
- not specified
- Details on results:
- No further information
- Results with reference substance (positive control):
- No information available
- Reported statistics and error estimates:
- No information available
- Sublethal observations / clinical signs:
The 96 -hour LC50 of tributyl phosphate in rainbow trout (Oncorhynchus mykiss) was 13mg/L.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96h LC50 of tributyl phosphate in rainbow trout (Oncorhynchus mykiss) was 13 mg/l.
- Executive summary:
The 96 -hour LC50 of tributyl phosphate in rainbow trout (Oncorhynchus mykiss) was 13 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Older proprietary GLP study similar to guidelines, summaried in OECD SIDS (secondary source). Officially accepted and peer-reviewed by the OECD SIDS evaluation programme.
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- yes
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant - Analytical monitoring:
- no
- Details on sampling:
- No information available
- Vehicle:
- not specified
- Details on test solutions:
- Static fresh water
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Oncorhynchus mykiss, rainbow trout
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information available
- Hardness:
- No information available
- Test temperature:
- No information available
- pH:
- No information available
- Dissolved oxygen:
- No information available
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Nominal
- Details on test conditions:
- No further information available
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- NOEC
- Effect conc.:
- 4.9 mg/L
- Nominal / measured:
- nominal
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Duration:
- 24 h
- Dose descriptor:
- LC50
- Effect conc.:
- 13 mg/L
- Nominal / measured:
- nominal
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- nominal
- Details on results:
- The 96h LC50 was 11mg/l
- Results with reference substance (positive control):
- No information
- Reported statistics and error estimates:
- No information available
- Sublethal observations / clinical signs:
No further information available
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96-h LC50 of tributyl phosphate in rainbow trout was 11 mg/l.
- Executive summary:
The 96-hour LC50 of tributyl phosphate in rainbow trout was 11 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- data from handbook or collection of data
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Fish acute toxicity (96 hour exposure)
- GLP compliance:
- no
- Remarks:
- : literature study
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant. - Analytical monitoring:
- yes
- Details on sampling:
- A static water test system was used to examine the stability of tributyl phosphate in water alone, and in beakers containing fish. Samples were collected at various time points and analysed for tributyl phosphate.
- Vehicle:
- not specified
- Details on test solutions:
- A 3-4 ppm concentration of tributyl phosphate was prepared in water, in 2 L beakers. The temperature was maintained at 25 °C. The beakers were not aerated.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- Oryzias latipes, killifish, weighing 0.1-0.2 g were purchased from a market.
Fish were acclimated to the test conditions for at least 10 days at 25 °C.
Fish were maintained throughout the test in groups of 7-9. The fish were not fed during the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information available
- Hardness:
- No information available
- Test temperature:
- 25 °C
- pH:
- No information available
- Dissolved oxygen:
- No information available
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Measured (GC)
- Details on test conditions:
- Dilution water was tap water used after dechlorination by passage through an activated charcoal column.
Tests took place in 1 L test solution. The beakers were not aerated. - Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 9.6 mg/L
- Nominal / measured:
- meas. (not specified)
- Basis for effect:
- mortality (fish)
- Details on results:
- The 96-hour LC50 of tributyl phosphate to Oryzias latipes was 9.6 mg/L.
- Results with reference substance (positive control):
- Not relevant.
- Reported statistics and error estimates:
- No information available
- Sublethal observations / clinical signs:
The 96-hour LC50 of tributyl phosphate, to Oryzias latipes was 9.6 mg/L.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 96-hour LC50 of tributyl phosphate, to Oryzias latipes was 9.6 mg/L.
- Executive summary:
The LC50 of tributyl phosphate was determined using killifish in a static test. The 96-hour LC50 of tributyl phosphate, to Oryzias latipes was 9.6 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Published study.
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- 96 hour acute toxicity to fish
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant. - Analytical monitoring:
- yes
- Details on sampling:
- A static water test system was used to examine the stability of tributyl phosphate in water alone, and in beakers containing fish. Samples were collected at various time points and analysed for tributyl phosphate.
- Vehicle:
- not specified
- Details on test solutions:
- A 3-4 ppm concentration of tributyl phosphate was prepared in water, in 2 L beakers. The temperature was maintained at 25 °C. The beakers were not aerated.
- Test organisms (species):
- Carassius auratus
- Details on test organisms:
- Carassius auratus, Goldfish, weighing 0.8 - 2.8 g were purchased from a market.
Fish were acclimated to the test conditions for at least 10 days at 25 °C.
Fish were maintained throughout the test in groups of 7-9. The fish were not fed during the test. - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information
- Hardness:
- No information provided.
- Test temperature:
- 25 °C
- pH:
- No information provided.
- Dissolved oxygen:
- No information provided.
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Measured
- Details on test conditions:
- Dilution water was tap water used after dechlorination by passage through an activated charcoal column.
Tests took place in 7 L test solution. The beakers were not aerated. - Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.8 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- not specified
- Basis for effect:
- mortality (fish)
- Details on results:
- The 96-hour LC50 of tributyl phosphate to Carassius auratus was 8.8mg/l.
- Results with reference substance (positive control):
- Not relevant.
- Reported statistics and error estimates:
- No information available
- Sublethal observations / clinical signs:
The 96-hour LC50 of tributyl phosphate to Carassius auratus was 8.8 mg/L.
- Conclusions:
- The 96-hour LC50 of tributyl phosphate to Carassius auratus was 8.8 mg/L.
- Executive summary:
The LC50 of tributyl phosphate was determined using Goldfish in a static test.
The 96 hour LC50 of tributyl phosphate to Carassius auratus was 8.8 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Published peer reviewed study summarising results for 200 chemicals
- Qualifier:
- according to guideline
- Guideline:
- other: DEV, L15 (1976)
- Principles of method if other than guideline:
- Acute fish toxicity
- GLP compliance:
- no
- Remarks:
- : published study
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant - Analytical monitoring:
- no
- Details on sampling:
- No information available
- Vehicle:
- not specified
- Details on test solutions:
- Static freshwater
- Test organisms (species):
- Leuciscus idus
- Details on test organisms:
- Leuciscus idus, the Ide.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information available
- Hardness:
- No information available
- Test temperature:
- No information available
- pH:
- No information available
- Dissolved oxygen:
- No information available
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Nominal
- Details on test conditions:
- No information available
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC0
- Effect conc.:
- 5.8 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 7.6 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Duration:
- 96 h
- Dose descriptor:
- LC100
- Effect conc.:
- 8.7 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The 96-hour LC50 was 7.6 mg/L.
- Results with reference substance (positive control):
- No information
- Reported statistics and error estimates:
- No information
- Sublethal observations / clinical signs:
No further information
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96-hour LC50 of tributyl phosphate in Leuciscus idus was 7.6 mg/L.
- Executive summary:
The authors summarise the acute toxicity of 200 chemicals to fish. The 96-hour LC50 of tributyl phosphate in Leuciscus idus was found to be 7.6 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Published peer reviewed study.
- Qualifier:
- according to guideline
- Guideline:
- other: Japanese Industrial Standard: JIS K 0102-1986-71 "Testing methods for industrial waste water"
- Principles of method if other than guideline:
- The study was performed to the Japanese Industrial Standard (JIS K 0102-1986-71): Testing methods for industrial waste water
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant. - Analytical monitoring:
- not specified
- Details on sampling:
- No inforamtion provided
- Vehicle:
- no
- Details on test solutions:
- The dilution water used for the test was taken from underground water pumped up from the ground of Kurume Research Laboratories. The quality of dilution water used for the test were confirmed to meet the ministerial ordinance of the Ministry of Health and Welfare in total hardness and evaporated residue. The other analysed items was confirmed to meet the water quality criteria for fisheries.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- Oryzias latipes, orange-red killifish, were used as the test species. The fish used were taken from Nakashima fish farm, Daimyojin Nagasu-cho Tamana-gun, Kumamoto 869-01, Japan.
At reception, fish showing abnormal signs were removed. The fish were removed according to a flow-through system for 3 -5 weeks after external disinfection. External disinfection was carried out according to static conditions for about 24 hours in an aqueous solution containing 20 mg/L of Elbarju and 7 g/L sodium chloride.
Fish were reared in an acclimitisation tank according to a flow-through system at temperature of 25 ± 2 °C for about 28 days. During this period, abnormal fish were removed.
Fish of the same lot which had passed the HgCl2 test were subjected to the test. - Test type:
- other: static or semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- No information available
- Hardness:
- Stated as measured continuously but no values provided.
- Test temperature:
- 25 ± 2°C
- pH:
- Stated as measured continuously but no values provided.
- Dissolved oxygen:
- Stated as measured continuously but no values provided.
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Two nominal concentrations tested
- Details on test conditions:
- The test was carried out in a round glass vessel. There was 4 L of test water per vessel. There were 10 fish per test concentration. They were exposed in a static or semi-stative system. The test water was renewed every 8 - 16 hours.
- Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 14.2 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Details on results:
- No further details on results provided.
- Results with reference substance (positive control):
- No information provided
- Reported statistics and error estimates:
- The 48-hour LC50 value was estimated by Doudoroff method or Probit method.
- Sublethal observations / clinical signs:
No further information available.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 48-hour LC50 for tributyl phosphate in Oryzias latipes was 14.2mg/l.
- Executive summary:
The 48 hour LC50 for tributyl phosphate in Oryzias latipes was 14.2 mg/L, as determined in a static/semi-static test.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Published study similar to guidelines
- Qualifier:
- equivalent or similar to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant - Analytical monitoring:
- yes
- Details on sampling:
- Samples weer taken from fish exposure tanks at approximately mid-depth at 0 and 96 hours and one of each duplicate exposure chamber at 24, 48 and 72 hours for tests completed before 1st December 1981. Tests completed after this date (exposures were no longer duplicated) were sampled at 0, 24, 48, 72 and 96 hours in all exposure chambers.
- Vehicle:
- not specified
- Details on test solutions:
- Test water was dechlorinated City of Superior, WI water
- Test organisms (species):
- Pimephales promelas
- Details on test organisms:
- Adult fathead minnows, aged approximately 26-37 days old. Test fish were not fed for 24 hours prior to, or during, the test.
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- Not examined
- Hardness:
- 41.3 - 58.6mg/L
- Test temperature:
- 25.9 and 26.7 °C
- pH:
- 6.5-8.2 (test water)
- Dissolved oxygen:
- No information available
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Measured
- Details on test conditions:
- Fish loading did not exceed 0.5 g/L-1 in flow-through systems. No further information
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 7.79 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: Test temp: 25.9 °C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.18 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Test temp: 25.9 °C
- Duration:
- 96 h
- Dose descriptor:
- EC50
- Effect conc.:
- 6.56 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- behaviour
- Remarks on result:
- other: Test temp: 26.7 °C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 11 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Remarks on result:
- other: Test temp: 26.7 °C
- Details on results:
- No further information
- Results with reference substance (positive control):
- Not relevant
- Reported statistics and error estimates:
- LC50 and EC50 calculated using Trimmed Spearman-Karber Method with 95% confidence intervals
- Sublethal observations / clinical signs:
The 96 -hour EC50 and LC50 of tributyl phosphate to fathead minnows was 7.79 and 8.18 mg/L at a test temperature of 25.9 °C, respectively, and 6.56 and 11 mg/L at a test temperature of 26.7 °C, respectively.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96 hour EC50 and LC50 of tributyl phosphate to fathead minnows was 7.79 and 8.18 mg/L at a test temperature of 25.9°C, respectively, and 6.56 and 11 mg/L at a test temperature of 26.7°C, respectively.
- Executive summary:
The acute toxicity of tributylphosphate was investigated in the fathead minnow. LC50 values of 8.18 -11 mg/L and EC50 values of 6.56 -7.78 mg/L are reported.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Published reference; officially accepted and peer-reviewed by the OECD SIDS evaluation programme
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 203 (Fish, Acute Toxicity Test)
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant. - Analytical monitoring:
- not specified
- Details on sampling:
- No information available.
- Vehicle:
- not specified
- Details on test solutions:
- Test solutions were prepared from the substance and dechlorinated drinking water in 5 concentrations equally spaced by a factor of 1.8. There were 10 fish in a 2 L test solution. In some cases a vehicle (dimethylsulfoxide) and/or dispersant (HCO-40) were used, but the substances for which this was the case were not specified.
Controls were prepared from water and the maximum concentrations of vehicle and dispersant used. - Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- Oryzias latipes, red killifish, were obtained from the market and acclimated for 10 days in dechlorinated drinking water.
The batch of fish whose mortality was below 5 % in a week was used for the test.
The test was conducted without feeding. - Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information available
- Hardness:
- Approximately 40mg/L.
- Test temperature:
- 20 ± 1°C.
- pH:
- 7.2
- Dissolved oxygen:
- The concentration of dissolved oxygen was kept more than 60 % of the saturation value.
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Five nominal concentrations were used equally spaced by a factor of 1.8 for each concentration.
- Details on test conditions:
- The test was carried out by the semi-static method, with 10 fish held in a 2 L test solution without feeding.
- Reference substance (positive control):
- no
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- nominal
- Basis for effect:
- mortality (fish)
- Details on results:
- The 96-hour LC50 of tributyl phosphate in Oryzias latipes was 17mg/L.
- Results with reference substance (positive control):
- Not relevant.
- Reported statistics and error estimates:
- No information available
- Sublethal observations / clinical signs:
The 96-hour LC50 of tributyl phosphate in Oryzias latipes was 17 mg/L.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96-hour LC50 of tributyl phosphate in Oryzias latipes was 17mg/L.
- Executive summary:
The LC50 of tributyl phosphate was determined using killifish in a semi-static test maintained at a temperature of 20 ± 1 °C. The fish were obtained from the market and allowed to acclimatise for 10 days prior to testing. Only batches with a mortality of lower than 5 % in a week were used for testing. Five different concentrations of the substance were used; each concentration differed by a factor of 1.8. Each concentration was tested with 10 fish in a 2l solution. The LC50 was determined after 96 hours of exposure, and found to be 17 mg/L.
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Published study
- Reason / purpose for cross-reference:
- reference to same study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Assessment of acute toxicity in Oryzias latipes
- GLP compliance:
- no
- Remarks:
- : literature study
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
not applicable - Analytical monitoring:
- yes
- Details on sampling:
- no data
- Vehicle:
- yes
- Details on test solutions:
- A supersaturated solution of a chemical was agitated for 2 hours at over 20 °C and left for 2 hours at 20 ° C in order to determine solubility. The water phase was filtered with filter paper for analysis. The no-octanol/water partition coefficient was determined by dissolving a chemical in distilled water at a concentration of 1-4 mol/L. n-Octanol or water, respectively, was added so that the ratio of n-octanol/water was 0.2 and it was agitated at 20 °C or 2 hours. Both phases, were then separated and filtered for analysis. The concentration of the chemical in the water and n-octanol phases was determined by gas chromatography with a 3-m x 2-mm glass column or by ultraviolet spectrophotometry.
- Test organisms (species):
- Oryzias latipes
- Details on test organisms:
- Oryzias latipes was obtained from the market and acclimated for at least 1 week i ndechlorinated water at 20 °C. The LC50 was determined by exposing 10 Oryzias latipes to 2 litres of a chemical solution. Detials on test conditions below.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Post exposure observation period:
- no data
- Hardness:
- 80 mg/L
- Test temperature:
- 20 ± 1 °C
- pH:
- no data
- Dissolved oxygen:
- no data
- Salinity:
- no data
- Nominal and measured concentrations:
- no data
- Details on test conditions:
- The LC50 was determiend by exposing 10 Oryzias latipes (3 cm long and 0.3g weight), to 2 litres of a chemical solution at 20 ± 1 °C for 48-hours with the 8 hours dark and 16 hours light. The oxygen uptake rate was determiend by putting 10 Orizias latipes in an Erlenmeyer flask (3-litre) filled with test solution which was saturated with air, and the flask was sealed with an electrode. Then it was left without aeration at 20 °C for 4 hours. The concentration of dissolved oxygen eas measured every 30 minutes.
- Reference substance (positive control):
- not specified
- Duration:
- 48 h
- Dose descriptor:
- LC50
- Effect conc.:
- 1.3 mg/L
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- mortality (fish)
- Details on results:
- The LC50 (48-hours) for Oryzias latipes was 1.3 mg/L.
- Results with reference substance (positive control):
- No data
- Reported statistics and error estimates:
- No data
- Sublethal observations / clinical signs:
The 48-hour LC50 of tributylphosphate in Oryzias latipes was found to be 1.3 mg/L under the conditions of this study.
- Validity criteria fulfilled:
- not applicable
- Conclusions:
- The 48 -hour LC50 of tributylphosphate in Oryzias latipes was found to be 1.3 mg/L under the conditions of this study.
- Executive summary:
The acute toxicity of a number of chemicals were assessed in a 48-hour test in Oryzias latipes. Under the conditions of this study, the LC50 of tributylphosphate was found to be 1.3 mg/L
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Published peer reviewed study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Acute toxicity test in fish
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant - Analytical monitoring:
- yes
- Details on sampling:
- No information available
- Vehicle:
- not specified
- Details on test solutions:
- No information available
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Salmo gairdneri, rainbow trout. Only 4 fish were used per concentration.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information available
- Hardness:
- No information available
- Test temperature:
- 15 °C
- pH:
- No information available
- Dissolved oxygen:
- No information available
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Nominal and measured concentrations are listed in the OECD SIDS
- Details on test conditions:
- Static freshwater
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 11.5 - <= 13.5 mg/L
- Nominal / measured:
- nominal
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- >= 5 - <= 9 mg/L
- Nominal / measured:
- meas. (not specified)
- Details on results:
- The nominal LC50 was 11.5-13.5mg/l.
- Results with reference substance (positive control):
- Not relevant
- Reported statistics and error estimates:
- No information available
- Sublethal observations / clinical signs:
The 96 -hour LC50 of tributyl phosphate in rainbow trout was 11.5 -13.5 mg/L (nominal concentrations).
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96 hour LC50 of tributyl phosphate in rainbow trout was 11.5 -13.5 mg/l (nominal concentrations).
- Executive summary:
The 96-hour LC50 of tributyl phosphate in rainbow trout was 11.5 -13.5 mg/L (nominal concentrations).
- Endpoint:
- short-term toxicity to fish
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Published peer reviewed study
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Acute toxicity test in fish
- GLP compliance:
- not specified
- Specific details on test material used for the study:
- Details on properties of test surrogate or analogue material:
Not relevant - Analytical monitoring:
- no
- Details on sampling:
- No information provided
- Vehicle:
- not specified
- Details on test solutions:
- Static freshwater
- Test organisms (species):
- Oncorhynchus mykiss (previous name: Salmo gairdneri)
- Details on test organisms:
- Salmo gairdeneri, rainbow trout, estuarine fish freshwater.
- Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 96 h
- Post exposure observation period:
- No information available
- Hardness:
- No information available
- Test temperature:
- 20, 15 or 10 °C
- pH:
- No information available
- Dissolved oxygen:
- No information available
- Salinity:
- Not relevant
- Nominal and measured concentrations:
- Nominal
- Details on test conditions:
- No further information
- Reference substance (positive control):
- not specified
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 4.2 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: Test temp: 20 °C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 8.2 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: Test temp: 15 °C
- Duration:
- 96 h
- Dose descriptor:
- LC50
- Effect conc.:
- 11.8 mg/L
- Nominal / measured:
- nominal
- Remarks on result:
- other: Test temp: 10 °C
- Details on results:
- The 96h LC50 of tributyl phosphate to rainbow trout was 4.2, 8.2 and 11.8 mg/L at test temperatures of 20, 15 and 10 °C, respectively.
- Results with reference substance (positive control):
- Not relevant
- Reported statistics and error estimates:
- No information available
- Sublethal observations / clinical signs:
The 96 -hour LC50 of tributyl phosphate to rainbow trout was 4.2, 8.2 and 11.8 mg/L at test temperatures of 20, 15 and 10 °, respectively.
- Validity criteria fulfilled:
- not specified
- Conclusions:
- The 96-hour LC50 of tributyl phosphate to rainbow trout was 4.2, 8.2 and 11.8mg/l at test temperatures of 20, 15 and 10 °C, respectively
- Executive summary:
The 96 -hour LC50 of tributyl phosphate to rainbow trout was 4.2, 8.2 and 11.8 mg/L at test temperatures of 20, 15 and 10 °C, respectively
Referenceopen allclose all
Description of key information
A large number of studies are available in various fish species.
Key value for chemical safety assessment
Fresh water fish
Fresh water fish
- Dose descriptor:
- LC50
- Effect concentration:
- 4.2 mg/L
Additional information
The acute toxicity of tributylphosphate has been investigated in a number of studies in various fish species. The results of the studies are summarised below. The lowest and thus most conservative LC50 value (Dave et al, 1979, 96h-LC50: 4.2 mg/L) was chosen as key value for chemical safety assessment.
Species | LC50 (mg/L) | Time (h) | Comment | Reference |
Oryzias latipes | 9.6 | 96 |
| Sasaki et al, 1981a |
Carassius auratus | 8.8 | 96 |
| Sasaki et al, 1981b |
Leuciscus idus
| 7.6 | 96 |
| Juhnke & Luedemann, 1978 |
Oryzias latipes | 14.2 | 48 |
| CITI, 1992 |
Pimephales promelas | 8.18-11 | 96 |
| Geiger et al, 1986 |
Oryzias latipes | 17 | 96 |
| Yoshioka & Ose, 1993 |
Oryzias latipes | 5.85-18.5 | 24-48 | Effects of temperature | Tsuko et al, 1985 |
Oryzias latipes | 1.3 | 48 |
| Yoshioka et al, 1985 |
Oncorhynchus mykiss | 13 | 96 |
| Bowman & Schrier, 1990 |
Oncorhynchus mykiss | 11 | 96 |
| ABC Labs, 1978 |
Salmo gairdneri | 11.5-13.5 | 96 |
| Dave & Lidman, 1978 |
Pimephales promelas | 6.4 | 96 |
| ABC Labs, 1978 |
Salmo gairdneri | 4.2-11.8 | 96 | Effects of temperature | Dave et al, 1979 |
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