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Toxicological information

Direct observations: clinical cases, poisoning incidents and other

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Administrative data

Endpoint:
direct observations: clinical cases, poisoning incidents and other
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: scientifically acceptable and well documented

Data source

Reference
Reference Type:
publication
Title:
Organophosphate exposure inhibits non-specific esterase staining in human blood monocytes
Author:
Mandel JS, Berlinger NT, Kay N, Connett J, Reape M
Year:
1989
Bibliographic source:
Am. J. Ind. Med. 15, 207-212 (1989)

Materials and methods

Study type:
study with volunteers
Endpoint addressed:
not applicable
Principles of method if other than guideline:
The objective of this study was to investigate the association between exposure to tributyl phosphate and monocyte nonspecific esterase staining activity in workers from a plant. A cross-sectional study design was used to identify the exposed plant employees, and both a plant and a general population group. In addition to completing a questionaire for each participant, 30 cc of blood were drawn and analyzed using 4 techniques, 2 morphological and 2 enzymatic. These procedures included aTtechnicon D-90 autoanalyzer, a Technicon H-6000 autoanalyzer, a manual enzymatic using alpha napththyl butyrate, and a manual morphologic using Wright-Giesma stain.
GLP compliance:
no

Test material

Constituent 1
Chemical structure
Reference substance name:
Tributyl phosphate
EC Number:
204-800-2
EC Name:
Tributyl phosphate
Cas Number:
126-73-8
Molecular formula:
C12H27O4P
IUPAC Name:
tributyl phosphate
Details on test material:
no data

Method

Subjects:
4 study groups were identified, 3 from within the plant and 1 from outside the plant. These included a group exposed to triaryl phosphate, a group exposed to tributyl phosphate, an unexposed plant control group, and a general population control group.
Reason of exposure:
unintentional, occupational
Exposure assessment:
estimated

Results and discussion

Clinical signs:
no data

Any other information on results incl. tables

RM-Freetext:
Some decrease in nonspecific esterase straining of monocytes in occupational exposed persons.

Applicant's summary and conclusion

Executive summary:

The objective of this study was to investigate the association between exposure to tributyl phosphate and monocyte nonspecific esterase staining activity in workers from a plant. A cross-sectional study design was used to identify the exposed plant employees, and both a plant and a general population group. In addition to completing a questionaire for each participant, 30 cc of blood were drawn and analyzed using 4 techniques, 2 morphological and 2 enzymatic. These procedures included aTtechnicon D-90 autoanalyzer, a Technicon H-6000 autoanalyzer, a manual enzymatic using alpha napththyl butyrate, and a manual morphologic using Wright-Giesma stain.

Some decrease in nonspecific esterase straining of monocytes in occupational exposed persons.