Tributyl phosphate

Genetic toxicity: in vitro

Administrative data

Endpoint:

in vitro gene mutation study in bacteria

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No OECD guideline or GLP defined.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1985

Materials and methods

Principles of method if other than guideline:

other: Ames Test (Ames et al. 1973a, 1975)

GLP compliance:

not specified

Type of assay:

bacterial reverse mutation assay

Test material

Test material information

Constituent 1

Reference substance name:

Tributyl phosphate

EC Number:

204-800-2

EC Name:

Tributyl phosphate

Cas Number:

126-73-8

Molecular formula:

C12H27O4P

IUPAC Name:

tributyl phosphate

Details on test material:

Purity: 99,8 %

Method

Target gene:

ames assay: detection of base pair substitutions and frameshift mutations

Species / strain

Species / strain / cell type:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Additional strain / cell type characteristics:

not specified

Metabolic activation:

with and without

Metabolic activation system:

S9 mix

Test concentrations with justification for top dose:

see: Any other information on materials and methods

Controls

Untreated negative controls:

yes

Negative solvent / vehicle controls:

yes

True negative controls:

not specified

Positive controls:

yes

Positive control substance:

other: see: any other information on materials and methods incl. tables

Details on test system and experimental conditions:

Ames test

Results and discussion

Test results

Species / strain:

S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Metabolic activation:

with and without

Genotoxicity:

negative

Cytotoxicity / choice of top concentrations:

other: Doses up to and including 120 µg per plate did not cause any bacteriotoxic effects

Vehicle controls validity:

valid

Untreated negative controls validity:

valid

Positive controls validity:

valid

Remarks on result:

other: other: S. typhimurium TA 1535, TA 1537, TA 98 and TA 100

Remarks:

Migrated from field 'Test system'.

Any other information on results incl. tables

Genotoxicity: negative

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information):
negative

Executive summary:

The test substance Tri-n-butylphosphate was investigated in the Salmonella/microsome test for point-mutagenic effects in doses up to 12500 µg per plate on four Salmonella typhimurium LT2 mutants. These were the histidine-auxotropic strains TA 1535, TA 100; TA 1537 and TA 98.

Doses up to and including 120 µg per plate did not cause any bacteriotoxic effects. The total bacteria counts remained unchanged. No inhibition of growth was observed. In doses above this the substance had a strong bacteriotoxic effect specific to strain, so that this range can only be used to a limited extent up to 500 µg per plate for evaluation purposes.

Evidence of mutagenic activity for the test substance was not found. Neither a dose-related doubling of the mutant count nor a biologically relevant increase in the same, in comparison with the negative controls, was observed.

The positive controls sodiumazide, nitrofurantoine, 4 -nitro-o-phenylenediamine and 2 -aminoanthracene had a marked mutagenic effect, as can be seen from the biologically relevant increase of mutant colonies, compared with the corresponding negative control.

The test substance was found to be negative for genotoxicity.