Tributyl phosphate

Specific investigations: other studies

Administrative data

Endpoint:

endocrine system modulation

Type of information:

experimental study

Adequacy of study:

supporting study

Reliability:

4 (not assignable)

Rationale for reliability incl. deficiencies:

documentation insufficient for assessment

Data source

Reference

Reference Type:

publication

Title:

Potential estrogenic effects of phosphorus-containing flame retardants.

Author:

Zhang Q, Lu M, Dong X, Wang C, Zhang C, Liu W, Zhao M

Year:

2014

Bibliographic source:

Environ Sci Technol. 48(12), 6995-7001

Materials and methods

Principles of method if other than guideline:

The agonistic/antagonistic activity of a group of phosphorus-containing flame retardants (PFRs) is examined by three in vitro models (luciferase reporter gene assay, yeast two-hybrid assay, and E-screen assay). Molecule docking was used to further explain the interactions between ERα and PFRs.

Type of method:

in vitro

Test material

Test material information

Constituent 1

Reference substance name:

Tributyl phosphate

EC Number:

204-800-2

EC Name:

Tributyl phosphate

Cas Number:

126-73-8

Molecular formula:

C12H27O4P

IUPAC Name:

tributyl phosphate

Results and discussion

Details on results:

in the in vitro luciferase reporter gene assay no REC20 of Tributyl phosphate for ERα could be determined, i.e. no activity to E2. Only for potential estrogenic effects in the E-Screen assay absolute numbers were given (10-7 M for TBP and 10-9 M for E2).

Any other information on results incl. tables

The agonistic/antagonistic activity of a group of phosphorus-containing flame retardants (PFRs) is examined by three in vitro models (luciferase reporter gene assay, yeast two-hybrid assay, and E-screen assay). Molecular docking was used to further explain the interactions between ERα and PFRs. The authors state that TBP does not behave as an ERα agonist or antagonist. However in the E-Screen Assay a very low binding affinity occurred at 10-7 M.