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Toxicological information

Eye irritation

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Administrative data

Endpoint:
eye irritation: in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No GLP defined.

Data source

Referenceopen allclose all

Reference Type:
study report
Title:
Unnamed
Year:
1986
Reference Type:
publication
Title:
Acute Toxicologic Evaluation of Tri-n-butylphosphate
Author:
Bomhard E, Pauluhn J, Ruf J
Year:
2000
Bibliographic source:
Int J Toxicol 19, 338

Materials and methods

Test guideline
Qualifier:
according to guideline
Guideline:
OECD Guideline 405 (Acute Eye Irritation / Corrosion)
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Tributyl phosphate
EC Number:
204-800-2
EC Name:
Tributyl phosphate
Cas Number:
126-73-8
Molecular formula:
C12H27O4P
IUPAC Name:
tributyl phosphate
Details on test material:
Purity: 99,80 %

Test animals / tissue source

Species:
rabbit
Strain:
New Zealand White

Test system

Vehicle:
unchanged (no vehicle)
Controls:
other: The test animals served also as control ( the other eye was not treated)
Amount / concentration applied:
100 µl
Duration of treatment / exposure:
24 h
Observation period (in vivo):
21 d
Number of animals or in vitro replicates:
3 female rabbits

Results and discussion

In vivo

Resultsopen allclose all
Irritation parameter:
cornea opacity score
Basis:
mean
Time point:
other: 1, 24, 48, 72 hours
Score:
0.4
Max. score:
4
Reversibility:
fully reversible within: 7 days
Irritation parameter:
iris score
Basis:
mean
Time point:
other: 1, 24, 48, 72 hours
Score:
0.2
Max. score:
2
Reversibility:
fully reversible within: 3 days
Irritation parameter:
conjunctivae score
Basis:
mean
Time point:
other: 1, 24, 48, 72 hours
Score:
1.5
Max. score:
3
Reversibility:
not fully reversible within: 14 days
Remarks on result:
other: reddening
Irritation parameter:
chemosis score
Basis:
mean
Time point:
other: 1, 24, 48, 72 hours
Score:
1
Max. score:
4
Reversibility:
fully reversible within: 14 days

Any other information on results incl. tables

Irritation in eye:

 Animal Nr., sex (body weight)  examined organ  Symptoms                   DRAIZE-Grade after  Irritation Value
       1h*  24h  48h  72h  7d  14d 21d   
 E14, female (3,1 kg)  opacity of cornea    1  1  1  0  0  0  0,7
   cornea area    2  1  1  0  0  -  
   iris    1  0  0  0  0  0  -  0,0
   conjunctivae  R  2  2  1  0  0  0  1,0
     S  2  1  0  0  0  0  -  0,3
     T  2  0  0  0  0  0  -  
 E15, female (3,5 kg)   opacity of cornea    1  1  1  1  0  0  -  1,0
    cornea area    3  3  3  3  0  0  -  
     iris    1  0  0  0  0  0  -  0,0
    conjunctivae  R  2  2  2  2  1  0  -  2,0
     S  3  2  1  1  1  0  -  1,3
     T  2  1  0  0  0  0  -  
 E17, female (3,1 kg)   opacity of cornea    0  0  0  0  0  -  0,0
    cornea area    0  0  0  0  0  0  
    iris    0  0  0  0  0  0  -  0,0
    conjunctivae  R  2  2  1  0  0  0  -  1,0
     S  2  0  0  0  0  0  -  0,0
     T  1  0  0  0  0  -

*= only the values after 24h were taken in consideration!

R = reddening

S = swelling

- = not examined

Applicant's summary and conclusion

Interpretation of results:
slightly irritating
Remarks:
Migrated information
Executive summary:

The test substance was tested in a 24 h eye irritation test in rabbits.

The test substance (100 µl) was administered into one eye of 3 rabbits (the other eye remained untreated and served as control). The substance was washed out after 24 hours with NaCl.

The animals were observed for 21 days after experiment.

The test substance was found to be slightly irritating to eye.