Tributyl phosphate

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1986

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: No OECD guideline or GLP defined.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1986

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

other: EG-Richtlinie 84/449 (Amtsblatt der Europäischen Gemeinschaften 27, 1984, L 251, 96)

GLP compliance:

not specified

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

Tributyl phosphate

EC Number:

204-800-2

EC Name:

Tributyl phosphate

Cas Number:

126-73-8

Molecular formula:

C12H27O4P

IUPAC Name:

tributyl phosphate

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: 9 (males) to 14 (females) weeks
- Weight at study initiation: 165 g for males and 163 g for females
- Fasting period before study: 16 h
- Housing: 5 animals per cage
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22 +- 2
- Humidity (%): 50 +- 10
- Photoperiod (hrs dark / hrs light): 12/12

IN-LIFE DATES: From: 1986-14-01 To: 1986-02-24

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

unchanged (no vehicle)

Doses:

1.3; 1.5; 1.8; 2,.0; 3.1 mL/kg bw

No. of animals per sex per dose:

5/sex/dose

Control animals:

not specified

Details on study design:

body weight was determined before application, one week later and at the end of the 14-day observation period

Statistics:

after Rosiello et al., J. Tox. Environ. Health 3, 797, 1977

Results and discussion

Effect levels

Dose descriptor:

LD50

Effect level:

1 552 mg/kg bw

Mortality:

1,5 ml/kg bw: 2 male/ 2 female
1,8 ml/kg bw: 3 male/ 4 female
2,0 ml/kg bw: 4 male/ 4 female
3,1 ml/kg bw: 5 male/ 5 female

Clinical signs:

other: Clinical signs were observed in dose groups: 1,5; 1,8; 2,0; 3,1 ml/kg bw.

Gross pathology:

No findings in dose group 1,3 ml/kg.
In dose group 1,5 ml/kg 4 rats (2 male/2 female) showed effects on gastric mucosa.
In the 1,8 ml/kg dose group 3 male animals showed effects on gastric mucosa and 4 female animals had effects on gastric mucosa, inflated intestine and reddened stomach.
In the 2,0 ml/kg dose group 4 male and 4 female animals showed effects on gastric mucosa, inflated intestines and a reddened lung. In the dose group of 3,1 ml/kg all animals (5male/ 5 female) showed effects on gastric mucosa, inflated intestines and a reddened lung.

Any other information on results incl. tables

After a single oral dose of 1,5 up to 3,1 ml/kg bw following symptoms could be observed in the test animals:

Diminished general condition; narcosis, bloody eye margins, face down position or lateral position, ruffled fur.

Applicant's summary and conclusion

Executive summary:

Tributyl phosphate was tested in a single dose experiment in male and female Wistar rats.

The doses tested were: 1,3; 1,5; 1,8; 2,0; 3,1 ml/kg bw.

Signs of intoxication were: diminuated general condition; narcose, bloody eye margins, face down position or lateral position, ruffled fur.

The LD50 was: 1,6 ml/kg bw for male and female rats.