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EC number: 204-800-2 | CAS number: 126-73-8
Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1989
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 990
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- Qualifier:
- according to guideline
- Guideline:
- other: EG-Richtlinie 84/449/EWG B.2.
- Qualifier:
- according to guideline
- Guideline:
- other: TSCA-Guideline §798.1150
- GLP compliance:
- yes
- Test type:
- traditional method
- Limit test:
- no
Test material
- Reference substance name:
- Tributyl phosphate
- EC Number:
- 204-800-2
- EC Name:
- Tributyl phosphate
- Cas Number:
- 126-73-8
- Molecular formula:
- C12H27O4P
- IUPAC Name:
- tributyl phosphate
- Details on test material:
- Purity: 99,62 %
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Winkelmann, Borchen, Germany
- Age at study initiation: 2-3 months
- Weight at study initiation: 166 to 210 g
- Fasting period before study: none
- Housing: 5 animals per cage
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period: at least 5 days
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 22+- 2
- Humidity (%): 40-50
- Air changes (per hr): 10
- Photoperiod (hrs dark / hrs light): 12/12
IN-LIFE DATES: 1989
Administration / exposure
- Route of administration:
- inhalation: aerosol
- Type of inhalation exposure:
- nose/head only
- Vehicle:
- other: unchanged (no vehicle)
- Analytical verification of test atmosphere concentrations:
- yes
- Duration of exposure:
- 4 h
- Concentrations:
- 0, 511, 801, 2140, and 4242 mg/m³ air
- No. of animals per sex per dose:
- 5/sex/dose
- Control animals:
- yes
Results and discussion
Effect levels
- Dose descriptor:
- LC50
- Effect level:
- > 4.242 mg/L air
- Exp. duration:
- 4 h
- Mortality:
- 2 male rats in dose group 4242 mg/L air died.
- Clinical signs:
- other: Group 3 ( 801 mg/L air) showed piloerection, diminuated motility, difficulties in breathing, bloody snout. Group 4 (2140 mg/L air) showed piloerection, untended fur, diminuated motility, nose secretion, sniffing sounds, bradypnoe, complications in breath
- Body weight:
- 3 days after experiment in the male rats of group 2 (511 mg/L air) a retardation in weight gain and in groups 3 to 5 (801; 2140; 4242 mg/m³ air) a loss of weight could be observed.
- Gross pathology:
- died intercurrently: inflated, liver-like lung; red-crusted nose; anemic spleen and kidneys; changed liver signing; gastrointestinal trakt filled with yellow mucus.
Animals killed after observation period: inflated lung; effects in spleen (anemic, reduced in volume, enlarged, dark coloured); changed liver signing; inflated intestine.
Any other information on results incl. tables
Acute toxicity: inhalation: aerosol
| N | Concentration nomin. analyt. mg/m³ air | Toxicological result | Duration of symptoms | Timepoint od death | Particle < 5 µm (%) | |||
| Male rat | ||||||||
| 1 | air-control | 0* | 0** | 5*** | - | - | - | |
| 2 | 2425 | 511 | 0 | 0 | 5 | - | - | 100 |
| 3 | 9700 | 801 | 0 | 5 | 5 | 4h-6h | - | 100 |
| 4 | 13400 | 2140 | 0 | 5 | 5 | 4h-7h | - | 100 |
| 5 | 48500 | 4242 | 2 | 5 | 5 | 4h-28d | 2d | 100 |
| Female rat | ||||||||
| 1 | air-control | 0 | 0 | 5 | - | - | - | |
| 2 | 2425 | 511 | 0 | 0 | 5 | - | - | 100 |
| 3 | 9700 | 801 | 0 | 5 | 5 | 4h-6h | - | 100 |
| 4 | 19400 | 2140 | 0 | 5 | 5 | 4h-4d | - | 100 |
| 5 | 48500 | 4242 | 0 | 5 | 5 | 4h-7d | - | 100 |
LC50 male: approx. 4242 mg/m ³ air.
N= Group number
4h= direktly after exposition
*= Number of mortalities
**= Number of animals with symptoms
***= Number of animals used
Applicant's summary and conclusion
- Executive summary:
Acute inhalative toxicity was tested with the test substance in male and female Wistar rats.
The doses tested for 4 hours were: 0; 511; 801; 2140; 4242 mg/m³ air.
Observed symptoms: diminuated motility, ataxie, adynamia in hindpaw, prostration, piloerection, untended fur, nose secretion, sniffing sounds, difficulties in breathing, bradypnoe, breathing sounds, chromodacryorrhoe, red-coloured urine, inflated abdomen, bloddy snout, loss of myotactic reflex, reduction of body weight. These symptoms point to a strong adverse effect to the respiratory tract.
After 4242 mg/m³ air 2 of 5 male rats died; the female rats in this dose group showed no mortalities.
The LD 50 was approx. 4242 mg/m³ air in the the more sensitive male rat.
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