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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Proprietary guideline study, cited in OECD SIDS. Officially accepted and peer-reviewed by the OECD SIDS evaluation programme.
Qualifier:
according to guideline
Guideline:
EPA OTS 795.1200 (Gammarid Acute Toxicity Test)
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
not specified
Details on sampling:
No information available
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
other aquatic crustacea: Hyalella azteca
Details on test organisms:
The Gammarid Hyalella azteca, length 1-2mm
Test type:
flow-through
Water media type:
not specified
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
No information available
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
No information available
Nominal and measured concentrations:
Nominal
Details on test conditions:
No information available
Reference substance (positive control):
not specified
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
< 1.9 mg/L
Nominal / measured:
nominal
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
2.4 mg/L
Nominal / measured:
nominal
Details on results:
No further information available
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 96-hour NOEC of tributyl phosphate to the Gammarid Hyalella azteca was < 1.9 mg/L, and the 96 hour LC50 was 2.4 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The 96 hour NOEC of tributyl phosphate to the Gammarid Hyalella azteca was < 1.9 mg/L, and the 96 hour LC50 was 2.4mg/L.
Executive summary:

The 96-hour NOEC of tributyl phosphate to theGammarid Hyalella aztecawas < 1.9 mg/L, and the 96 hour LC50was 2.4 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Proprietary GLP study, summarised in OECD SIDS (secondary source). Officially accepted and peer-reviewed by the OECD SIDS evaluation programme.
Qualifier:
no guideline followed
Principles of method if other than guideline:
Daphnia acute toxicity test
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
yes
Details on sampling:
No information available
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna
Test type:
flow-through
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
No information available
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
No information available
Nominal and measured concentrations:
No information available
Details on test conditions:
No information available
Reference substance (positive control):
not specified
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
0.75 mg/L
Nominal / measured:
not specified
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.6 mg/L
Nominal / measured:
not specified
Details on results:
No further information
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 48 -hour EC50 of tributyl phosphate in Daphnia was 2.6 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The 48 hour EC50 of tributyl phosphate in Daphnia was 2.6 mg/l.
Executive summary:

The 48 -hour EC50of tributyl phosphate inDaphniawas 2.6 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Proprietary GLP study similar to guidelines, summarised in OECD SIDS (secondary source). Officially accepted and peer-reviewed by the OECD SIDS evaluation programme.
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
no
Details on sampling:
No details provided
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna
Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
No information available
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
No information available
Nominal and measured concentrations:
Nominal
Details on test conditions:
No information available
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
NOEC
Effect conc.:
1.8 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
9 mg/L
Nominal / measured:
nominal
Details on results:
No further information
Results with reference substance (positive control):
Not relevant
Reported statistics and error estimates:
No information available

The 48-hour NOEC of tributyl phosphate in Daphnia magna was 1.8 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The 48h NOEC of tributyl phosphate in Daphnia magna was 1.8mg/L.
Executive summary:

The 48 -hour NOEC of tributyl phosphate in Daphnia magna was 1.8 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study
Qualifier:
no guideline followed
Principles of method if other than guideline:
Daphnia magna acute immobilisation
GLP compliance:
no
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant.
Analytical monitoring:
not specified
Details on sampling:
No information available.
Vehicle:
not specified
Details on test solutions:
No information available.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna Straus; standardised test strain IRCHA
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
No information available.
Hardness:
No information available.
Test temperature:
No information available.
pH:
No information available.
Dissolved oxygen:
No information available.
Salinity:
No information available.
Nominal and measured concentrations:
Not relevant
Details on test conditions:
No information available.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
30 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 25-36
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
41 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
The 24 hour EC50 value for tributyl phosphate in Daphnia is 30 mg/L.
Results with reference substance (positive control):
Not relevant.
Reported statistics and error estimates:
No information available.

The 24-hour EC50 value for tributyl phosphate in Daphnia is 30 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The 24 hour EC50 value for tributyl phosphate in Daphnia is 30 mg/L.
Executive summary:

The acute toxicity of tributyl phosphate was investigated in a 96-hour study in Daphnia magna. The 24 hour EC50 value for tributyl phosphate in Daphnia was found to be 30 mg/L under the conditions of this study.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
22 August 1985 to 23 August 1885
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Laboratory data
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
not specified
Details on sampling:
No information provided
Vehicle:
no
Details on test solutions:
No information provided
Test organisms (species):
Daphnia magna
Details on test organisms:
The test organsims were 6 - 24 hours old. They were from Laborzucht der Bayer AG Leverkusen.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
No information provided
Hardness:
No information provided
Test temperature:
20 - 22 °C
pH:
after 24 hours, the pH ranged from 7.8 to 8.4
Dissolved oxygen:
after 24 hours, O2 ranged from 8.5 - 9.0 mg/L
Salinity:
No information provided
Nominal and measured concentrations:
Main study 1: nominal concentrations used were 2.5, 5.0, 7.5, 10.0, 20.0, 30.0, 40.0, 50.0, 75 and 100
Main study 2: nominal concentrations used were 5, 7.5, 10, 20, 30, 40, 50, 75 and 1000 mg/L.
Details on test conditions:
The test was carried out in cylindrical test vessels that contained 10 mL of the test solution. No aeration was used in the test. It was a static system. No solubiliser was used. There were 5 daphnia per test vessel.
Reference substance (positive control):
yes
Remarks:
Potassium dichromate
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Pre-test
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
5.8 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Main study 1
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
28.75 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: Main study 2
Details on results:
No further information
Results with reference substance (positive control):
The EC50 was 2.38 mg/L.
Reported statistics and error estimates:
No information provided

EC50 values of between 5 -28.75 mg/L are reported in this series of studies.

Validity criteria fulfilled:
yes
Conclusions:
EC50 values of between 5 -28.75 mg/L are reported in this series of studies.
Executive summary:

The acute toxicity of tributylphosphate was investigated in a series of studies in Daphnia magna. 96 -hour EC50 values of between 5 -28.75 mg/L are reported.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study
Qualifier:
no guideline followed
Principles of method if other than guideline:
24-hour acute toxicity to Daphnia magna, using a standardised procedure
GLP compliance:
no
Remarks:
: literature study
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
No information available.
Analytical monitoring:
not specified
Details on sampling:
No information available.
Vehicle:
not specified
Details on test solutions:
No information available.
Test organisms (species):
Daphnia magna
Details on test organisms:
24 hour old animals from a clone of Daphnia magna
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
No information available.
Hardness:
16° (German)
Test temperature:
20°C - 22°C
pH:
7.6 - 7.7
Dissolved oxygen:
saturated with oxygen
Salinity:
Not relevant
Nominal and measured concentrations:
No information available.
Details on test conditions:
Tap water free from chlorine
Reference substance (positive control):
not specified
Duration:
24 h
Dose descriptor:
LC0
Effect conc.:
7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
33 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
24 h
Dose descriptor:
LC100
Effect conc.:
58 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No further information available.
Results with reference substance (positive control):
No information available.
Reported statistics and error estimates:
No information available.

The 24 hour LC50 of tributyl phosphate in Daphnia magna was 33 mg/L under the conditions of this study.

Validity criteria fulfilled:
not specified
Conclusions:
The 24 hour LC50 of tributyl phosphate in Daphnia was 33 mg/L under the conditions of this study.
Executive summary:

The authors describe the assessment of a number of chemicals in a standardised test for acute toxicity in Daphnia magna. Under the conditions of this assay, the 24 hour EC50 of tributyl phosphate in Daphnia was found to be 33 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Study period:
21st June to 25th June 1991
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
EPA OTS 795.1200 (Gammarid Acute Toxicity Test)
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
yes
Details on sampling:
Tributyl phosphate was measured 48 hours before test initiation and at 0, 48 and 96 hours by gas-liquid chromatography.
Control, vehicle blank and tributyl phosphate-fortified water samples were analysed at each time period.
Vehicle:
yes
Details on test solutions:
A diluted stock solution was prepared at a concentration of 50 mg/ml in dimethyl formamide.
Test organisms (species):
Gammarus sp.
Details on test organisms:
Gammarus pseudolimnaeus were collected from a spring branch in Missouri and transferred to ABC Laboratories. The spring water in which the gammarids were collected in was gradually diluted with ABC hard-blended test water. The gammarids were housed in glass aquaria, and cultured in aged hard maple leaves, variously under static and flow-through conditions. Temperature of the water was approximately 15-20 °C. The gammarids were fed at least once a week with a suspension of fish food, cereal leaves and alfalfa mixed with algae. They were held under a 16 hour light/8 hour dark photoperiod, with 30 minute dusk and dawn transitions. The organisms were transferred from culture on the day of test initiation. Test organisms were selected from a 17 °C flow-through subculture. Juveniles 2-3mm long were isolated by gently rinsing culture leaves in a glass tray.
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Post exposure observation period:
No information provided.
Hardness:
160-190 mg/L hardness as CaCO3
Test temperature:
The test chambers were immersed in a water bath maintained at 18 °C ± 2 °C
pH:
8.0-8.2
Dissolved oxygen:
8.0-8.3 mg/L
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal concentrations: 0.3, 0.6, 1.3, 2.5, and 5.0 mg tributyl phosphate per litre test water.
Mean measured concentrations (gas-liquid chromatography) in filtered samples at 96 hours were: 0.28, 0.52, 1.2, 3.0 and 5.3 mg/L.
Details on test conditions:
A 0.5 L proportional diluter system with syringe dispenser was used to introduce dilution water and tributyl phosphate compound into test chambers. Flow splitting chambers were used to thoroughly mix and divide each test concentration for delivery to the test chambers. Turbulence was minimised by introducing influent water through 14-gauge needles.
Test chambers consisted of 1 L glass beakers covered with 50 mesh stainless steel screen. A small piece of nylon netting was provided as artificial substrate for the organism.
Dilution water used was ABC hard-blended water, delivered to each test chamber at a rate of ~250 mL/chamber/hour. Alkalinity of dilution water: 182-190 mg/L (as CaCO3). Conductivity of dilution water: 360-370µMhos/cm.
The test system was on a 16 hour light/8 hour dark photoperiod with a 30 minute dusk and dawn transition period.
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
> 5.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
LC50
Remarks:
(Probit)
Effect conc.:
4.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 4.0-5.8 mg/L
Duration:
72 h
Dose descriptor:
LC50
Remarks:
(moving average)
Effect conc.:
2 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 1.6-2.5 mg/l
Duration:
96 h
Dose descriptor:
LC50
Remarks:
(moving average)
Effect conc.:
1.7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: 1.4-2.2 mg/L
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
> 5.3 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
48 h
Dose descriptor:
EC50
Remarks:
(Probit method)
Effect conc.:
4.6 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 4.0-5.8 mg/L
Duration:
72 h
Dose descriptor:
EC50
Remarks:
(moving average)
Effect conc.:
1.7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 1.4-2.2 mg/L
Duration:
96 h
Dose descriptor:
EC50
Remarks:
(Binomial method)
Effect conc.:
1.7 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: 1.2-3.0 mg/L
Duration:
96 h
Dose descriptor:
NOEC
Effect conc.:
0.52 mg/L
Nominal / measured:
meas. (arithm. mean)
Conc. based on:
test mat.
Basis for effect:
other: all effects
Details on results:
Abnormal effecs of mortality, lethargy, quiescence, immobility, and/or organisms tending to the bottom of the test chamber were observed in the 1.2, 3.0 and 5.3 mg/L test levels.
Results with reference substance (positive control):
Not reported
Reported statistics and error estimates:
LC50 values and 95% confidence limits calculated by using the binomial, moving average and probit tests, where data permitted.

The 96 hour EC50 and LC50 values for tributyl phosphate for Gammarus pseudolimnaeus were calculated to be 1.7 mg/L. The 96 hour NOEC was found to be 0.52 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The 96 hour EC50 and LC50 values for tributyl phosphate for Gammarus pseudolimnaeus were calculated to be 1.7 mg/L. The 96 hour NOEC was found to be 0.52 mg/L.
Executive summary:

The acute toxicity of tributyl phosphate to Gammarus pseudolimneaus was determined in a flow through test with duplicate nominal test concentrations of 0.3, 0.6, 1.3, 2.5, and 5.0 mg/L. Abnormal effecs of mortality, lethargy, quiescence, immobility, and/or organisms tending to the bottom of the test chamber were observed in the 1.2, 3.0, and 5.3 mg/L test levels. The 96 hour EC50 and LC50 values for tributyl phosphate for Gammarus pseudolimnaeus were calculated to be 1.7 mg/L. The 96 hour NOEC was found to be 0.52 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study
Qualifier:
according to guideline
Guideline:
other: ISO 1975
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
not specified
Details on sampling:
No details provided
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna neonates
Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
72 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
No information available
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
No information available
Nominal and measured concentrations:
Nominal
Details on test conditions:
No further information available
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
12.8 mg/L
Nominal / measured:
nominal
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
3.65 mg/L
Nominal / measured:
nominal
Duration:
72 h
Dose descriptor:
EC50
Effect conc.:
2.1 mg/L
Nominal / measured:
nominal
Details on results:
No further information
Results with reference substance (positive control):
Not relevant
Reported statistics and error estimates:
No information available

The 24 hour EC50 of tributyl phosphate in Daphnia magna was 12.8 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The 24 hour EC50 of tributyl phosphate in Daphnia was 12.8mg/l.
Executive summary:

The 24 hour EC50 of tributyl phosphate in Daphnia was 12.8 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published study
Qualifier:
no guideline followed
Principles of method if other than guideline:
Daphnia acute toxicity
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
not specified
Details on sampling:
No information available
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna
Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
No information available
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
No information available
Nominal and measured concentrations:
Nominal (assumed; no information available)
Details on test conditions:
No information available
Reference substance (positive control):
not specified
Duration:
6 h
Dose descriptor:
EC50
Effect conc.:
52 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
35 mg/L
Nominal / measured:
nominal
Details on results:
No further information available
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 6 hour EC50 of tributyl phosphate in Daphnia was 52 mg/L, and the 24 hour EC50 was 35 mg/L.

Conclusions:
The 6 hour EC50 of tributyl phosphate in Daphnia was 52mg/l, and the 24 hour EC50 was 35mg/l.
Executive summary:

The 6 hour EC50of tributyl phosphate inDaphniawas 52 mg/L, and the 24 hour EC50was 35 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published study
Qualifier:
no guideline followed
Principles of method if other than guideline:
Daphnia acute toxicity
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
not specified
Details on sampling:
No information available
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Daphnia pulex
Details on test organisms:
Daphnia pulex
Test type:
not specified
Water media type:
not specified
Limit test:
no
Total exposure duration:
24 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
No information available
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
No information available
Nominal and measured concentrations:
Nominal (assumed; no information available)
Details on test conditions:
No information available
Reference substance (positive control):
not specified
Duration:
6 h
Dose descriptor:
EC50
Effect conc.:
93 mg/L
Nominal / measured:
nominal
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
68 mg/L
Nominal / measured:
nominal
Details on results:
No further information available
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 6-hour EC50 of tributyl phosphate in Daphnia pulex was 93 mg/L, and the 24 hour EC50 was 68 mg/L.

Conclusions:
The 6 hour EC50 of tributyl phosphate in Daphnia pulex was 93mg/l, and the 24 hour EC50 was 68mg/l.
Executive summary:

The 6-hour EC50of tributyl phosphate in Daphnia pulex was 93 mg/L, and the 24 hour EC50was 68 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published literature study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
no guideline followed
Principles of method if other than guideline:
Assessmnet of acute aquatic toxicity using Moina macrocopa
GLP compliance:
no
Remarks:
: literature study
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
yes
Details on sampling:
no data
Vehicle:
yes
Details on test solutions:
A supersaturated solution of a chemical was agitated for 2 hours at over 20 °C and left for 2 hours at 20 ° C in order to determine solubility. The water phase was filtered with filter paper for analysis. The no-octanol/water partition coefficient was determined by dissolving a chemical in distilled water at a concentration of 1-4 mol/L. n-Octanol or water, respectively, was added so that the ratio of n-octanol/water was 0.2 and it was agitated at 20 °C or 2 hours. Both phases, were then separated and filtered for analysis. The concentration of the chemical in the water and n-octanol phases was determined by gas chromatography with a 3-m x 2-mm glass column or by ultraviolet spectrophotometry.
Test organisms (species):
other: Moina macrocopa
Details on test organisms:
The culture medium for breeding Moina macrocopa was prepared by mixing liquids A and B in the ratio of 9:1, and was neutralised by NaHCO3 before use. Liquid A was prepared by boiling 1 L of distilled water with 4 g of rice straw for 2 hours, and after filtration it was left for 24 hours to increase bacteria. Liquid B was a soil extract prepared by the same method as the liquid A. Moina macrocopa was cultured in the medium, and those of about 5 days old were used.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
3 h
Post exposure observation period:
No data
Hardness:
No information provided
Test temperature:
20 ± 1 °C
pH:
No information provided
Dissolved oxygen:
No information provided
Salinity:
No information provided
Nominal and measured concentrations:
No information provided
Details on test conditions:
The culture medium for breeding Moina macrocopa was prepared by mixing liquids A and B in the ratio of 9:1, and was neutralised by NaHCO3 before use. Liquid A was prepared by boiling 1 L of distilled water with 4 g of rice straw for 2 hours, and after filtration it was left for 24 hours to increase bacteria. Liquid B was a soil extract prepared by the same method as the liquid A. Moina macrocopa was cultured in the medium, and those of about 5 days old were used. Ten Moina macrocopa in 100 mL of test solution was put in a 250 mL vail vessel at 20 ± 1 °C and the survivors were counted after 3 hours of exposure to determien the LC50.
Reference substance (positive control):
not specified
Duration:
3 h
Dose descriptor:
LC50
Effect conc.:
1.8 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
No further details on the results
Results with reference substance (positive control):
Not relevant
Reported statistics and error estimates:
No information provided

The LC50 of the test material in Moina macropopa was found to be 1.8 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 of the test material in Moina macropopa was found to be 1.8 mg/L.
Executive summary:

The authors describe an assay to assess the acute toxicity of chemicals to the cladoceran, Moina macrocopa. The 3 -hour LC50 of tributylphosphate was found to be 1.8 mg/L.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published study
Reason / purpose for cross-reference:
reference to same study
Qualifier:
no guideline followed
Principles of method if other than guideline:
The authors describe a method to assess the acute toxicity of chemicals to the flatworm, Dugesia japonica
GLP compliance:
no
Remarks:
: published study
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not applicable
Analytical monitoring:
yes
Details on sampling:
No data
Vehicle:
yes
Details on test solutions:
A supersaturated solution of a chemical was agitated for 2 hours at over 20 °C and left for 2 hours at 20 ° C in order to determine solubility. The water phase was filtered with filter paper for analysis. The no-octanol/water partition coefficient was determined by dissolving a chemical in distilled water at a concentration of 1-4 mol/L. n-Octanol or water, respectively, was added so that the ratio of n-octanol/water was 0.2 and it was agitated at 20 °C or 2 hours. Both phases, were then separated and filtered for analysis. The concentration of the chemical in the water and n-octanol phases was determined by gas chromatography with a 3-m x 2-mm glass column or by ultraviolet spectrophotometry.
Test organisms (species):
other aquatic worm: Dugesia japonica
Details on test organisms:
The breeding liquid for Dugesia japonica was prepared bu dissolving 3.74 g of NaCl, 0.49 g KCL and 8.55 g of CaCl2 into distilled water to make 500 mL. This was diluted 100 times and neutralised by NaHCO3 befoer use. Dugesia japonica were collected from a stream around which there was no source of pollution and left with-out food for over 7 days in the breeding liquid to excrete alimentary canal contents. Those of about 2 cm long were used. Dugesia japonica was cut into two parts at the nearest section to the eyes of the trisected part between pharnyx and eyes. The body part was used for the regeneration test.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
7 d
Post exposure observation period:
No information provided
Hardness:
No information provided
Test temperature:
No information provided
pH:
No information provided
Dissolved oxygen:
No information provided
Salinity:
No information provided
Nominal and measured concentrations:
No information provided
Details on test conditions:
Test species of about 2 cm long were used. Dugesia japonica was cut into two parts at the nearest section to the eyes of the trisected part between pharnyx and eyes. The body part was used for the regeneration test. Ten body parts were put in 100 mL of a test solution, and this was left at 20 ± 1 °C for 7 days. Observation for head regeneration was carried out with a stereomicroscope on Days 3, 4, 5, 6 and 7 after head cutting, and the test solution was replaced at every observation.
Reference substance (positive control):
not specified
Duration:
7 d
Dose descriptor:
LC50
Effect conc.:
1 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
7 d
Dose descriptor:
EC50
Effect conc.:
0.6 mg/L
Nominal / measured:
meas. (not specified)
Conc. based on:
test mat.
Details on results:
No further information provided
Results with reference substance (positive control):
Not relevant
Reported statistics and error estimates:
No information provided

The LC50 was found to be 1.0 mg/L; the EC50 for head regeneration was found to be 0.6 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The LC50 was found to be 1.0 mg/L; the EC50 for head regeneration was found to be 0.6 mg/L.
Executive summary:

The authors describe a method for assessing the acute toxicity of chemicals to the flatworm, Dugesia japonica. Organisms were exposed for 7 days and mortality and head regeneration assessed. Unde the conditions of this assay, the LC50 for tributylphosphate was found to be 1.0 mg/L; the EC50 for head regeneration was found to be 0.6 mg/L.

Description of key information

A large number of studies, performed in a number of species, are available.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Dose descriptor:
EC50
Effect concentration:
1.8 mg/L

Additional information

The results of a large number of studies are available and are sumamrised below. In addition, the range-finding study for the 21 -day reproduction test, Kuehn et al (1989) reports 24 -hour LC0 and LC50 values for tributylphosphate in Daphnia magna of 9.3 and 35 mg/L respectively.


The lowest EC50-value reported by England & Cramer (1991, guideline study, reliability 1, LoA) was not chosen as key value for chemical safety assessment due to the fact that Gammarus pseudolimnaeus is not a standard test organism as advised in the OECD testing guidelines. The lowest and thus most conservative LC50 value for the standard organism Daphnia magna (ABC Laboratories, 1978, 48h-LC50: 1.8 mg/L) was chosen instead.


 



























































































Species



EC50 (mg/L)



Time (h)



Reference



Daphnia magna



30



96



Bringmann & Kuehn, 1982



Daphnia magna



5-28.75



96



Bayer, 1985



Daphnia magna



33



24



Bringmann & Kuehn, 1977



Gammarus pseudolimnaeus



1.7



96



England & Cramer, 1991



Daphnia magna



12.8



24



Dave et al, 1981



Daphnia magna



1.8



48



ABC Laboratories, 1978



Daphnia magna



52



6



Wakabayashi et al, 1988



35



24



Daphnia magna



2.6



48



ABC Laboratories, 1990



Daphnia pulex



6



93



Wakabayashi et al, 1988



24



68



Hyalella azteca



2.4



96



England & Schrier, 1990



Moina macrocopa



1.8



3



Yoshioda et al, 1986



Dugesia japonica



1.3



3