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Ecotoxicological information

Endpoint summary

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Additional information

Toxicity to fish

The acute toxicity of tributylphosphate has been investigated in a number of studies in various fish species. The results of the studies are summarised below.

Species

LC50 (mg/L)

Time (h)

Comment

Reference

Oryzias latipes

9.6

96

 

Sasaki et al, 1981a

Carassius auratus

8.8

96

 

Sasaki et al, 1981b

Leuciscus idus

 

7.6

96

 

Juhnke & Luedemann, 1978

Oryzias latipes

14.2

48

 

CITI, 1992

Pimephales promelas

8.18-11

96

 

Geiger et al, 1986

Oryzias latipes

17

96

 

Yoshioka & Ose, 1993

Oryzias latipes

5.85-18.5

24-48

Effects of temperature

Tsuko et al, 1985

Oryzias latipes

1.3

48

 

Yoshioka et al, 1985

Oncorhynchus mykiss

13

96

Data access unknown

Bowman & Schrier, 1990

Oncorhynchus mykiss

11

96

Data access unknown

ABC Labs, 1978

Salmo gairdneri

11.5-13.5

96

 

Dave & Lidman, 1978

Pimephales promelas

6.4

96

Data access unknown

ABC labs, 1978

Salmo gairdneri

4.2-11.8

 

Effects of temperature

Dave et al, 1979

Dave et al (1981) report a 7 -day LC50 of 11.4 mg/L and a 10 -Day NOEC of 13.5 mg/L in Danio rerio. The same authors report a 50 -day NOEC for the embryo and larval stages of Oncorhyncus mykiss of 18.3 mg/L. In a proprietary study summarised in the OECD SIDS, a NOEC of 0.82 is reported for a fish ELS toxicity test using Oncorhyncus mykiss.

Toxicity to aquatic invertebrates

The results of a large number of studies are available and are sumamrised below. In addition, the range-finding study for the 21 -day reproduction test, Kuehn et al (1989) reports 24 -hour LC0 and LC50 values for tributylphosphate in Daphnia magna of 9.3 and 35 mg/L respectively.

Species

EC50 (mg/L)

Time (h)

Comment

Reference

Daphnia magna

30

96

 

Bringmann & Kuehn, 1982

Daphnia magna

5-28.75

96

 

Bayer, 1985

Daphnia magna

33

24

 

Bringmann & Kuehn, 1977

Gammarus pseudolimnaeus

1.7

96

Data access unclear

England & Cramer, 1991

Daphnia magna

12.8

24

 

Dave et al, 1981

Daphnia magna

1.8

48

Data access unclear

ABC Laboratories, 1978

Daphnia magna

52

6

 

Wakabayashi et al, 1988

35

24

Daphnia magna

2.6

48

Data access unclear

ABC Laboratories, 1990

Daphnia pulex

6

93

 

Wakabayashi et al, 1988

24

68

Hyalella azteca

2.4

96

Data access unclear

England & Schrier, 1990

Moina macrocopa

1.8

3

 

Yoshioda et al, 1986

Dugesia japonica

1.3

3

 

 

A 21-day reproduction test was carried out in Daphnia magna to determine the NOEC for tributyl phosphate (Kuehn et al, 1989). . Evaluation parameters for fixing the NOEC were parent animal mortality, reproduction rate and the appearance of the first offspring during the test period. Animals were aged 24 hours at the start of the test. A semi-static procedure was used with open vessels maintained under fluorescent lighting, animals were fed daily with algae suspension (the test was carried out in accordance with an FEA guideline). The 24 hour EC50 value was also determined. The NOEC was found to be 1.3mg/l in the 21 day reproduction test.

The 21 day EC50 (immobilisation) of tributyl phosphate in Daphnia magna is also reported to be >2.1mg/l (ABC Laboratories, 1991).

Toxicity to aquatic invertebrates

The results of a large number of studies are available and are sumamrised below. In addition, the range-finding study for the 21 -day reproduction test, Kuehn et al (1989) reports 24 -hour LC0 and LC50 values for tributylphosphate in Daphnia magna of 9.3 and 35 mg/L respectively.

Species

EC50 (mg/L)

Time (h)

Comment

Reference

Daphnia magna

30

96

 

Bringmann & Kuehn, 1982

Daphnia magna

5-28.75

96

 

Bayer, 1985

Daphnia magna

33

24

 

Bringmann & Kuehn, 1977

Gammarus pseudolimnaeus

1.7

96

Data access unclear

England & Cramer, 1991

Daphnia magna

12.8

24

 

Dave et al, 1981

Daphnia magna

1.8

48

Data access unclear

ABC Laboratories, 1978

Daphnia magna

52

6

 

Wakabayashi et al, 1988

35

24

Daphnia magna

2.6

48

Data access unclear

ABC Laboratories, 1990

Daphnia pulex

6

93

 

Wakabayashi et al, 1988

24

68

Hyalella azteca

2.4

96

Data access unclear

England & Schrier, 1990

Moina macrocopa

1.8

3

 

Yoshioda et al, 1986

Dugesia japonica

1.3

3

 

 

A 21-day reproduction test was carried out in Daphnia magna to determine the NOEC for tributyl phosphate (Kuehn et al, 1989). . Evaluation parameters for fixing the NOEC were parent animal mortality, reproduction rate and the appearance of the first offspring during the test period. Animals were aged 24 hours at the start of the test. A semi-static procedure was used with open vessels maintained under fluorescent lighting, animals were fed daily with algae suspension (the test was carried out in accordance with an FEA guideline). The 24 hour EC50 value was also determined. The NOEC was found to be 1.3mg/l in the 21 day reproduction test.

The 21 day EC50 (immobilisation) of tributyl phosphate in Daphnia magna is also reported to be >2.1mg/l (ABC Laboratories, 1991).

Toxicity to microorganisms

A number of studies have been performed in various organisms and are summarised below.

Species

Endpoint

Time

Comment

Reference

Activated sludge

EC50: 100 mg/L

3h

 

Yoshioka et al, 1986

Pseudomonas putida

Toxicity threshold: >100 mg/L

16h

 

Bringmann & Kuehn, 1977

Tetrahymena pyriformis

EC 50: 20 mg/L

24h

 

Yoshioka, 1985

Activated sludge

EC50: 300 mg/L

3h

 

Bayer, 1985

Entosiphon sulcatum

Toxicity threshold: 14 mg/L

72h

 

(Bringmann & Kuehn, 1979)

Chilomonas paramecium

Toxicity threshold: 42 mg/L

48h

 

(Bringmann et al, 1980)

Uronema parduczi

Toxicity threshold: 21 mg/L

20h

 

(Bringmann & Kuehn, 1980)