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Long-term toxicity to fish

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Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Proprietary GLP guideline study, summarised in OECD SIDS (secondary source). Officially accepted and peer-reviewed by the OECD SIDS evaluation programme.
Qualifier:
according to guideline
Guideline:
other: EPA 1987 Fish Early Life Stage Toxicity Test 797.1600, Amended: 19064-19066
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
yes
Details on sampling:
No information available
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Rainbow trout Oncorhynchus mykiss
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
95 d
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
No information available
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
Not relevant
Nominal and measured concentrations:
Results based on measured concentrations
Details on test conditions:
No further information available
Reference substance (positive control):
not specified
Duration:
95 d
Dose descriptor:
NOEC
Effect conc.:
0.82 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
other: time to swim-up stage, survival, length, and weight.
Duration:
95 d
Dose descriptor:
LOEC
Effect conc.:
1.7 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
other: time to swim-up stage, survival, length, and weight.
Duration:
95 d
Dose descriptor:
other: MATC
Effect conc.:
1.2 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
other: time to swim-up stage, survival, length, and weight.
Details on results:
No further information available
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 95-day NOEC of tributyl phosphate in the fish early life stage toxicity test using rainbow trout was 0.82 mg/L (measured), the 95 -day LOEC was 1.7 mg/L (measured), and the 95-day MATC was 1.2 mg/L (measured).

Validity criteria fulfilled:
not specified
Conclusions:
The 95 day NOEC of tributyl phosphate in the fish early life stage toxicity test using rainbow trout was 0.82mg/l (measured), the 95 day LOEC was 1.7mg/l (measured), and the 95 day MATC was 1.2mg/l (measured).
Executive summary:

The 95 day NOEC of tributyl phosphate in the fish early life stage toxicity test using rainbow trout was 0.82 mg/L (measured), the 95 day LOEC was 1.7 mg/L (measured), and the 95 day MATC was 1.2 mg/L (measured).

Endpoint:
fish short-term toxicity test on embryo and sac-fry stages
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study
Qualifier:
no guideline followed
Principles of method if other than guideline:
Semistatic long term embryo-larval toxicity test
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
no
Details on sampling:
No details provided
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Embryo and larval stages of zebrafish, Brachydanio rerio. Fish were not fed during the test.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
10 d
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
25 °C
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal
Details on test conditions:
No further information available
Reference substance (positive control):
not specified
Duration:
10 d
Dose descriptor:
NOEC
Effect conc.:
13.5 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
not specified
Remarks on result:
other: threshold concentration
Details on results:
No further information
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 10 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of zebrafish was 13.5 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The 10 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of zebrafish was 13.5mg/L.
Executive summary:

The 10 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of zebrafish was 13.5 mg/L.

Endpoint:
fish short-term toxicity test on embryo and sac-fry stages
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study
Qualifier:
no guideline followed
Principles of method if other than guideline:
Semistatic long term embryo-larval toxicity test
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
no
Details on sampling:
No details provided
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Embryo and larval stages of estuarine rainbow trout, Salmo gairdneri. Fish were not fed during the test.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
10 d
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
8 °C
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal
Details on test conditions:
No further information available
Reference substance (positive control):
not specified
Duration:
50 d
Dose descriptor:
NOEC
Effect conc.:
8.3 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
not specified
Remarks on result:
other: threshold concentration
Details on results:
No further information
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 50 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of rainbow trout was 8.3 mg/L.

Conclusions:
The 50 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of rainbow trout was 8.3 mg/L.
Executive summary:

The 50 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of rainbow trout was 8.3 mg/L.

Endpoint:
fish, juvenile growth test
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study.
Qualifier:
according to guideline
Guideline:
other: ISO 1975
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
not specified
Details on sampling:
No information
Vehicle:
not specified
Details on test solutions:
No information
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Juvenile zebrafish, Brachydanio rerio.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
144 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
25 °C
pH:
7.3-8.5
Dissolved oxygen:
No information available
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal
Details on test conditions:
No further information available
Reference substance (positive control):
not specified
Duration:
144 h
Dose descriptor:
LC50
Effect conc.:
11.4 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
not specified
Details on results:
No further information
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 144 hour LC50 of tributyl phosphate to juvenile zebrafish was 11.4 mg/L.

Conclusions:
The 144 hour LC50 of tributyl phosphate to juvenile zebrafish was 11.4 mg/L.
Executive summary:

The 144 hour LC50 of tributyl phosphate to juvenile zebrafish was 11.4 mg/L.

Description of key information

A number of studies are available.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
NOEC
Effect concentration:
0.82 mg/L

Additional information

Dave et al (1981) report a 7 -day LC50 of 11.4 mg/L and a 10 -Day NOEC of 13.5 mg/L in Danio rerio. The same authors report a 50 -day NOEC for the embryo and larval stages of Oncorhyncus mykiss of 8.3 mg/L.


 


In a proprietary study summarised in the OECD SIDS, a NOEC of 0.82 mg/L is reported for a fish ELS toxicity test using Oncorhyncus mykiss. The reported lowest and thus most conservative NOEC value from this study was chosen as key value for chemical safety assessment.