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The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Ecotoxicological information

Long-term toxicity to fish

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Administrative data

Link to relevant study record(s)

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Endpoint:
fish early-life stage toxicity
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Proprietary GLP guideline study, summarised in OECD SIDS (secondary source). Officially accepted and peer-reviewed by the OECD SIDS evaluation programme.
Qualifier:
according to guideline
Guideline:
other: EPA 1987 Fish Early Life Stage Toxicity Test 797.1600, Amended: 19064-19066
GLP compliance:
yes
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
yes
Details on sampling:
No information available
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Rainbow trout Oncorhynchus mykiss
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
95 d
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
No information available
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
Not relevant
Nominal and measured concentrations:
Results based on measured concentrations
Details on test conditions:
No further information available
Reference substance (positive control):
not specified
Duration:
95 d
Dose descriptor:
NOEC
Effect conc.:
0.82 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
other: time to swim-up stage, survival, length, and weight.
Duration:
95 d
Dose descriptor:
LOEC
Effect conc.:
1.7 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
other: time to swim-up stage, survival, length, and weight.
Duration:
95 d
Dose descriptor:
other: MATC
Effect conc.:
1.2 mg/L
Nominal / measured:
meas. (not specified)
Basis for effect:
other: time to swim-up stage, survival, length, and weight.
Details on results:
No further information available
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 95-day NOEC of tributyl phosphate in the fish early life stage toxicity test using rainbow trout was 0.82 mg/L (measured), the 95 -day LOEC was 1.7 mg/L (measured), and the 95-day MATC was 1.2 mg/L (measured).

Validity criteria fulfilled:
not specified
Conclusions:
The 95 day NOEC of tributyl phosphate in the fish early life stage toxicity test using rainbow trout was 0.82mg/l (measured), the 95 day LOEC was 1.7mg/l (measured), and the 95 day MATC was 1.2mg/l (measured).
Executive summary:

The 95 day NOEC of tributyl phosphate in the fish early life stage toxicity test using rainbow trout was 0.82 mg/L (measured), the 95 day LOEC was 1.7 mg/L (measured), and the 95 day MATC was 1.2 mg/L (measured).

Endpoint:
fish short-term toxicity test on embryo and sac-fry stages
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study
Qualifier:
no guideline followed
Principles of method if other than guideline:
Semistatic long term embryo-larval toxicity test
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
no
Details on sampling:
No details provided
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Embryo and larval stages of zebrafish, Brachydanio rerio. Fish were not fed during the test.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
10 d
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
25 °C
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal
Details on test conditions:
No further information available
Reference substance (positive control):
not specified
Duration:
10 d
Dose descriptor:
NOEC
Effect conc.:
13.5 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
not specified
Remarks on result:
other: threshold concentration
Details on results:
No further information
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 10 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of zebrafish was 13.5 mg/L.

Validity criteria fulfilled:
not specified
Conclusions:
The 10 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of zebrafish was 13.5mg/L.
Executive summary:

The 10 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of zebrafish was 13.5 mg/L.

Endpoint:
fish short-term toxicity test on embryo and sac-fry stages
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study
Qualifier:
no guideline followed
Principles of method if other than guideline:
Semistatic long term embryo-larval toxicity test
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
no
Details on sampling:
No details provided
Vehicle:
not specified
Details on test solutions:
No information available
Test organisms (species):
Oncorhynchus mykiss (previous name: Salmo gairdneri)
Details on test organisms:
Embryo and larval stages of estuarine rainbow trout, Salmo gairdneri. Fish were not fed during the test.
Test type:
semi-static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
10 d
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
8 °C
pH:
No information available
Dissolved oxygen:
No information available
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal
Details on test conditions:
No further information available
Reference substance (positive control):
not specified
Duration:
50 d
Dose descriptor:
NOEC
Effect conc.:
8.3 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
not specified
Remarks on result:
other: threshold concentration
Details on results:
No further information
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 50 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of rainbow trout was 8.3 mg/L.

Conclusions:
The 50 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of rainbow trout was 8.3 mg/L.
Executive summary:

The 50 day NOEC (threshold concentration) of tributyl phosphate to embryo and larval stages of rainbow trout was 8.3 mg/L.

Endpoint:
fish, juvenile growth test
Type of information:
experimental study
Adequacy of study:
weight of evidence
Reliability:
4 (not assignable)
Rationale for reliability incl. deficiencies:
other: Published peer reviewed study.
Qualifier:
according to guideline
Guideline:
other: ISO 1975
GLP compliance:
not specified
Specific details on test material used for the study:
Details on properties of test surrogate or analogue material:
Not relevant
Analytical monitoring:
not specified
Details on sampling:
No information
Vehicle:
not specified
Details on test solutions:
No information
Test organisms (species):
Danio rerio (previous name: Brachydanio rerio)
Details on test organisms:
Juvenile zebrafish, Brachydanio rerio.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
144 h
Post exposure observation period:
No information available
Hardness:
No information available
Test temperature:
25 °C
pH:
7.3-8.5
Dissolved oxygen:
No information available
Salinity:
Not relevant
Nominal and measured concentrations:
Nominal
Details on test conditions:
No further information available
Reference substance (positive control):
not specified
Duration:
144 h
Dose descriptor:
LC50
Effect conc.:
11.4 mg/L
Nominal / measured:
nominal
Conc. based on:
not specified
Basis for effect:
not specified
Details on results:
No further information
Results with reference substance (positive control):
No information available
Reported statistics and error estimates:
No information available

The 144 hour LC50 of tributyl phosphate to juvenile zebrafish was 11.4 mg/L.

Conclusions:
The 144 hour LC50 of tributyl phosphate to juvenile zebrafish was 11.4 mg/L.
Executive summary:

The 144 hour LC50 of tributyl phosphate to juvenile zebrafish was 11.4 mg/L.

Description of key information

A number of studies are available.

Key value for chemical safety assessment

Fresh water fish

Fresh water fish
Dose descriptor:
NOEC
Effect concentration:
0.82 mg/L

Additional information

Dave et al (1981) report a 7 -day LC50 of 11.4 mg/L and a 10 -Day NOEC of 13.5 mg/L in Danio rerio. The same authors report a 50 -day NOEC for the embryo and larval stages of Oncorhyncus mykiss of 8.3 mg/L.


 


In a proprietary study summarised in the OECD SIDS, a NOEC of 0.82 mg/L is reported for a fish ELS toxicity test using Oncorhyncus mykiss. The reported lowest and thus most conservative NOEC value from this study was chosen as key value for chemical safety assessment.