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EC number: 204-465-2
CAS number: 121-33-5
this study, the biodegradation of Vanilline (initial concentration of
100 mg/L) was studied during
14 days according
to OECD guideline 301C. Inoculum used in the test was an
activated sludge at a concentration of 30 mg/L obtained by mixing 10
different samples from different city and industrial sewage plants,
soils and rivers in Japan. The percentage of biodegradation was
estimated by following 3 parameters: the Biological Oxygen Demand
(BOD), the Total Organic Carbon (TOC) and the test
substance analysis (estimated by HPLC). The corresponding
percentages of biodegradation obtained after 14 days were 97
%, 99 % and 100% respectively. Under the test conditions Vanillin is
therefore considered as readily biodegradable.
In a study (NIVA, 1996), the potential for
aerobic biodegradation of Vanillin was investigated by determination of
the biochemical oxygen demand (BOD5) and the chemical oxygen demand
(COD). The tests were performed in accordance with ISO 5815 method.
The BOD5/COD after 5 days was 0.72.
In these conditions, Vanillin is considered
as readily biodegradable.
Kinetic of testsubst.
2 day(s) = 71 %
4 day(s) = 9 %
6 day(s) = 10 %
The MITI study (1993) is selected as key study. Although GLP were not mentioned, this study was performed according to OECD 301C test guideline and was generated by the Japanese Competent Authorities. The vanillin biodegradation rate reached 97% (relative to biochemical oxygen demand) after 14 days.
Other studies were selected
as supporting studies: NIVA
study confirms the ready biodegradation potential of vanillin. Martinez
(1993), Healy (1979) and RP (1987) demonstrated the potential of
Vanillin for biodegradation in various conditions (anaerobic, effluents.
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