Registration Dossier
Registration Dossier
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EC number: 204-465-2 | CAS number: 121-33-5
Toxicity to soil microorganisms
Administrative data
- Endpoint:
- toxicity to soil microorganisms
- Type of information:
- experimental study
- Adequacy of study:
- disregarded due to major methodological deficiencies
- Reliability:
- 3 (not reliable)
- Rationale for reliability incl. deficiencies:
- other: Test conditions and methods poorly described, semi-quantitative and not validated method.
Data source
Reference
- Reference Type:
- publication
- Title:
- THE DESTRUCTION OF VANILLIN IN THE SOIL BY THE ACTION OF SOIL BACTERIA.
- Author:
- ROBBINS WJ; ELIZANDO AE
- Year:
- 1 918
- Bibliographic source:
- ALA. AGR. EXP. SB. BULL. 204. PP.125-31.
Materials and methods
- Principles of method if other than guideline:
- Amendement of soil with vanillin and soil microorganism quantification.
The effect of vanillin on microorganisms was assessed by measuring the number of microorganisms before and during the incubation period. - GLP compliance:
- no
Test material
- Reference substance name:
- Vanillin
- EC Number:
- 204-465-2
- EC Name:
- Vanillin
- Cas Number:
- 121-33-5
- Molecular formula:
- C8H8O3
- IUPAC Name:
- 1-butoxypropan-2-ol
- Details on test material:
- no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- no
Test substrate
- Vehicle:
- not specified
Test organisms
- Test organisms (inoculum):
- other: aerobic microorganisms
Study design
- Total exposure duration:
- 57 d
Test conditions
- Details on test conditions:
- The experiment was performed with 2 different soils (Alabama - ALB- and Arlington - ARL- , US). Alabama soil was characterised by the presence of vanillin-destroying bacteria, which were isolated and cultured in mineral media. Arlington soil is a heavy silty clay loam.
An experimental unit was composed by 9 Kg of air dry soil, placed in two-gallon pots and hydrated with distilled water. Each experimental condition was performed in duplicate. The experimental conditions were:
1- ALB
2- ALB + Vanillin [1g/kg]
3- ARL
4- ARL + Vanillin [1g/kg]
5- ARL + Vanillin [1g/kg] + suspension of pure culture of vanillin destroying bacteria
From time to time, the number of microorganisms was determined until 57 days of incubation.
Microorganism concentration determination:
The number of microorganisms was determined by the method described by Brown using albumen agar (no more details were provided by the
authors). The analysis was performed in duplicate using dilution of 1/20000 and 1/200000. - Reference substance (positive control):
- not specified
Results and discussion
- Details on results:
- no data
Applicant's summary and conclusion
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