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EC number: 204-465-2
CAS number: 121-33-5
Vanillin has been tested on the reproduction of Daphnia magna for 21 days according to the OECD202 and the GLP (NIVA, 1996). Test solutions were renewed 3 times per week. Vanillin concentration of 100 mg/L resulted in a lethal effect starting at day 3. No lethality was observed at 56 mg/L and lower concentrations during 21 days exposure. Sublethal effects of Vanillin included delayed occurence of the first brood, aborted broods and reduced number of live offspring. The EC50 for effect of reproduction (21 days) was estimated at 24 mg/L, LOEC was 18 mg/L and NOEC at 10 mg/L (all nominal concentartions). Since exposure concentrations were fluctuating, weighed mean concentrations were calculated and led to measured EC50 for effect of reproduction was 16 mg/L, NOEC at 5.9 mg/L and LOEC at 10 mg/L.
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