Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-428-0 | CAS number: 120-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Skin sensitisation
Administrative data
- Endpoint:
- skin sensitisation: in vivo (non-LLNA)
- Type of information:
- experimental study
- Adequacy of study:
- weight of evidence
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- documentation insufficient for assessment
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 1 971
- Report date:
- 1971
Materials and methods
Test guideline
- Qualifier:
- no guideline available
- Principles of method if other than guideline:
- A series of exposures was given to the male albino guinea-pigs over a three-week intervals; the first five guinea-pigs received nine topical applications of 0.05 mL varying concentrations (50% x 4, 75% x 5) in f.a.d. (13% guinea-pig fat in a 1:1 solution of acetone and dioxane) to clipped abraded skin, and the other five animals received four sacral intradermal injections of 0.1 mL 1% solution v/v of test material in DMP (dimethylphtalate). After a two-week rest period, the test animals were challenged for sensitization by applying 0.05 mL of 75% and 95% (v/v) concentrations to shaved intact shoulder skin. A group of 10 previously unexposed guinea-pigs received similar applications at the time of challenge to provide a direct comparison of the challenge reactions of skin of similar age.
- GLP compliance:
- no
- Type of study:
- not specified
- Justification for non-LLNA method:
- The study pre-dates LLNA method
Test material
- Reference substance name:
- 1,2,4-trichlorobenzene
- EC Number:
- 204-428-0
- EC Name:
- 1,2,4-trichlorobenzene
- Cas Number:
- 120-82-1
- Molecular formula:
- C6H3Cl3
- IUPAC Name:
- 1,2,4-trichlorobenzene
- Test material form:
- not specified
Constituent 1
In vivo test system
Test animals
- Species:
- guinea pig
- Strain:
- not specified
- Remarks:
- Albino
- Sex:
- male
- Details on test animals and environmental conditions:
- TEST ANIMALS
- Source: N/A
- Microbiological status of animals, when known: N/A
- Age at study initiation: N/A
- Weight at study initiation: 500 g
- Housing: N/A
- Diet (e.g. ad libitum): N/A
- Water (e.g. ad libitum): N/A
- Acclimation period: N/A
- Indication of any skin lesions: N/A
ENVIRONMENTAL CONDITIONS
- Temperature (°C): N/A
- Humidity (%): N/A
- Air changes (per hr): N/A
- Photoperiod (hrs dark / hrs light): N/A
- IN-LIFE DATES: N/A
Study design: in vivo (non-LLNA)
Inductionopen allclose all
- Route:
- other: epicutaneous, unknown (Group 1)
- Vehicle:
- other: f.a.d
- Concentration / amount:
- 50% x 4, 75% x 5
- Day(s)/duration:
- 9 days
- Adequacy of induction:
- not specified
- Route:
- other: intradermal (Group 2)
- Vehicle:
- other: DMP
- Concentration / amount:
- 1% / 0.1 mL x 4 days
- Day(s)/duration:
- 4 days
- Adequacy of induction:
- not specified
Challengeopen allclose all
- No.:
- #1
- Route:
- other: epicutaneous, unknown (Group 1 + 2)
- Vehicle:
- other: f.a.d.
- Concentration / amount:
- 75% / 0.05 mL
- Day(s)/duration:
- After a two-week rest period
- Adequacy of challenge:
- not specified
- No.:
- #1
- Route:
- other: epicutaneous, unknown (Group 1 + 2)
- Vehicle:
- other: f.a.d.
- Concentration / amount:
- 95% / 0.05 mL
- Day(s)/duration:
- After a two-week rest period
- Adequacy of challenge:
- not specified
- No. of animals per dose:
- 5 (Group 1) + 5 (Group 2)
- Details on study design:
- Cf. principles of method if other than guideline
- Positive control substance(s):
- no
Results and discussion
In vivo (non-LLNA)
Resultsopen allclose all
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 95%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: all 10 animals showed irritant reactions
- Key result
- Reading:
- 1st reading
- Hours after challenge:
- 24
- Group:
- test chemical
- Dose level:
- 75%
- No. with + reactions:
- 0
- Total no. in group:
- 10
- Remarks on result:
- other: all 10 animals showed irritant reactions
Any other information on results incl. tables
Table 7.4.1/1: Reactions on intact Guinea Pig Skin
| Test animals | Control animals | ||
Concentration (% in f.a.d.) | 75% | 95% | 75% | 95% |
Primary irritation test – 24 hrs | 4+, 6 neg. | 4+, 6 neg. | - | - |
Challenge test – 24 hrs | 3+++, 7+++ | 4+++, 6++ | 3+++, 7++ | 3+++, 7++ |
Applicant's summary and conclusion
- Interpretation of results:
- GHS criteria not met
- Conclusions:
- The test item did not cause skin sensitisation but produced irritation.
- Executive summary:
A series of exposures was given to the male albino guinea-pigs over a three-week intervals; the first five guinea-pigs received nine topical applications of 0.05 mL varying concentrations (50% x 4, 75% x 5) in f.a.d. (13% guinea-pig fat in a 1:1 solution of acetone and dioxane) to clipped abraded skin, and the other five animals received four sacral intradermal injections of 0.1 mL 1% solution v/v of test material in DMP (dimethylphtalate). After a two-week rest period, the test animals were challenged for sensitization by applying 0.05 mL of 75% and 95% (v/v) concentrations to shaved intact shoulder skin. A group of 10 previously unexposed guinea-pigs received similar applications at the time of challenge to provide a direct comparison of the challenge reactions of skin of similar age.
The test item did not cause skin sensitisation but produced irritation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
