1,2,4-trichlorobenzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Ûberprüfung der durchführbarkeit von Prüfungsvorschriften und der Aussagekraft der Grundprüfung des E. Chem. G.

Author:

Korte F and Greim H

Year:

1981

Bibliographic source:

Umweltforschungsplan des Umweltministeriums des Innern, Chemikalien, Forschungsbericht 107 04 006/01. 172

Reference 2

Reference Type:

secondary source

Title:

European Union Risk Assessment Report - 1,2,4-Trichlorobenzene

Author:

European Commission - European Chemicals Bureau

Year:

2003

Bibliographic source:

Office for Official Publications of the European Communities

Materials and methods

Test guideline

Qualifier:

according to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

Acclimation period not reported, detailed test results are not available

GLP compliance:

no

Remarks:

GLP was not mandatory at the time of the study

Test material

Test material information

Constituent 1

Reference substance name:

1,2,4-trichlorobenzene

EC Number:

204-428-0

EC Name:

1,2,4-trichlorobenzene

Cas Number:

120-82-1

Molecular formula:

C6H3Cl3

IUPAC Name:

1,2,4-trichlorobenzene

Details on test material:

- Name of test material (as cited in study report): 1,2,4-trichlorobenzene
- Analytical purity: 98%

Test animals

Species:

rabbit

Strain:

other: White Russian

Details on test animals or test system and environmental conditions:

TEST ANIMALS
- Weight at study initiation: 2.5-2.8 kg
- Housing: V2A-Steel cages
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum

ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 ± 3 °C
- Humidity (%): 60 ± 5 %
- Photoperiod (hrs dark / hrs light): 12 hrs dark / 12 hrs light

Test system

Type of coverage:

other: see "details on study design"

Preparation of test site:

shaved

Vehicle:

unchanged (no vehicle)

Controls:

not specified

Amount / concentration applied:

TEST MATERIAL
- Amount applied: 0.5 g

Duration of treatment / exposure:

4 hours

Observation period:

14 days. With the persistence of skin damage the examination period was extended until full recovery.

Number of animals:

6

Details on study design:

TEST SITE
In order to test the skin compatibility to 1,2,4-trichlorobenzene, the patch-test procedure was performed. 0.5 g of the test substance was applied on an approximately 6 cm² linen patch. The linen patch was covered with an equally large plastic film by means of a rubberized bandage (Leukoplast, P. BEIERSDORF AG & CO., Unna Straße 48, D-2000 Hamburg) and attached on the shaved skin of the back of the animals between the front and hind limbs. The linen patch stayed 4 hours on the skin. During this time the animals were kept in a rabbit-case, where although some freedom of movement was allowed, a rotation of the whole body was not possible. The head of the animals was not fixed in a special way.

SCORING SYSTEM: Draize score. For further details see "Any other information on materials and method incl. tables"

Results and discussion

In vivo

Results

Results 1

Irritation parameter:

erythema score

Basis:

mean

Remarks:

6 animals

Time point:

other: 30-60 min, 24, 48, 72, and 96 hours, 7 and 14 days

Score:

1

Max. score:

4

Reversibility:

not specified

Remarks on result:

other: The mean erythema score of 6 animals was measured at 30-60 min, 24, 48, 72, and 96 hours, 7 and 14 days

Results 2

Irritation parameter:

edema score

Basis:

mean

Remarks:

6 animals

Time point:

other: 30-60 min, 24, 48, 72, and 96 hours, 7 and 14 days

Score:

1

Max. score:

4

Reversibility:

not specified

Remarks on result:

other: The mean edema score of 6 animals was measured at 30-60 min, 24, 48, 72, and 96 hours, 7 and 14 days

Irritant / corrosive response data:

1,2,4-trichlorobenzene caused slight redness and slight oedema

Any other information on results incl. tables

Detailed results are not available

Applicant's summary and conclusion

Interpretation of results:

GHS criteria not met

Executive summary:

Korte & Greim (1981):

 

In order to test the skin compatibility to 1,2,4-trichlorobenzene, a the patch-test procedure was performed according to OECD guideline 404 with deviations (Analytical purity and acclimation period not reported, detailed test results are not available).

5 g of the test substance was applied on an approximately 6 cm² linen patch.

Detailed test results are not available. However the test substance substance caused slight redness and slight oedema. Thus no classification is indicated according to the GHS classification criteria for effects after a single exposure.