1,2,4-trichlorobenzene

Biodegradation in water and sediment: simulation tests

Administrative data

Endpoint:

biodegradation in water: simulation testing on ultimate degradation in surface water

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1980-1981

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable, well documented publication which meets basic scientific principles. (referring to Bartholomew and Pfaender, 1983)

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Influence of spatial and temporal variations on organic pollutant biodegradation rates in an estuarine environment.

Author:

Bartholomew GW and Pfaender FK

Year:

1983

Bibliographic source:

Applied and Environmental Microbiology, Vol. 45, No. 1, 103-109

Reference 2

Reference Type:

secondary source

Title:

1,2,4-Trichlorbenzol - BUA-Stoffbericht 17 - (Dezember 1987) [Report in German]

Author:

Beratergremium für umweltrelevante Altstoffe (BUA)

Year:

1987

Bibliographic source:

Wissenschaftliche Verlagsgesellschaft VCH, Weinheim, ISSN: 0179-2601

Materials and methods

Principles of method if other than guideline:

Biodegradation rates were assessed by heterotrophic uptake kinetics (Pfaender and Bartholomew, 1982, Appl. Environ. Microbiol. 44:159-164). This protocol measures both the uptake of radiolabeled substrate into microbial cells and subsequent respiration to 14CO2. After incubation, the 14CO2 produced from respiration was trapped from the headspace above acidified samples, and substrate uptake into cells was determined by filtering the water sample through membrane filters.

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1,2,4-trichlorobenzene

EC Number:

204-428-0

EC Name:

1,2,4-trichlorobenzene

Cas Number:

120-82-1

Molecular formula:

C6H3Cl3

IUPAC Name:

1,2,4-trichlorobenzene

Details on test material:

- Name of test material (as cited in study report): 1,2,4-trichlorobenzene
- Analytical purity: no data
- Specific activity (if radiolabelling): 13.1 mCi/mmol
- Locations of the label (if radiolabelling): 1,2,4-trichloro[U-14C]benzene

Radiolabelling:

yes

Study design

Inoculum or test system:

natural water

Duration of test (contact time):

>= 4 - <= 10 h

Results and discussion

Any other information on results incl. tables

summary of kinetic parameters for 1,2,4-trichlorobenzene

Date	Site	Temperature [°C]	Vmax^a) [ng * l^-1* h^-1]	Km^b)
11-7-80	Upstream	12	7.5	6.5
	Estuarine	14	<1	-
	Marine	17	<1	-
5-26-81	Upstream	24	<1	-
	Estuarine	28	7.9	7.0
	Marine	24	2.3	4.5

a) susbstrate uptake into cells. A V_max< 1 ng * l^-1* h^-1represents a metabolic rate less than that detectable by this technique.

b) CO²production from respiration