Registration Dossier
Registration Dossier
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-428-0 | CAS number: 120-82-1
Long-term toxicity to fish
Administrative data
Link to relevant study record(s)
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- other: EU Risk Assessment
- Adequacy of study:
- other information
- Reliability:
- other: EU Risk Assessment
- Rationale for reliability incl. deficiencies:
- other: no reliability is given as this is a summary entry for the EU RAR
- Principles of method if other than guideline:
- EU Risk Assessment
- GLP compliance:
- not specified
- Executive summary:
EU Risk Assessment, 2003:
Long-term studies on fish are all based on measured concentrations. The reported values for NOEC are close together. They range between 0.04 mg/l (Broecker et al., 1984) and 0.50 mg/l (Carlson and Kosian, 1987). All in EU RAR cited studies are considered to be valid.
The study on Zebrafish (Brachydanio rerio) was performed according to a UBA guideline (1982) in a dynamic system. The fish were 4 to 7 months old. The aquarium contained 36 l and the flow was 1.2 l/h. The concentrations were measured. LOEC was 0.18 mg/l (20% mortality and several behavioural abnormalities) (Broecker et al., 1984).
Suter and Rosen (1988) report the chronic MATC value to the marine fish Cyprinodon variegatus to be 0.222 mg/l. NOEC is estimated to be approximately half this value.
The lowest NOEC in long-term studies was 0.04 mg/l (mortality and behaviour, Zebra fish).
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- other: BUA report
- Adequacy of study:
- other information
- Reliability:
- other: BUA report
- Rationale for reliability incl. deficiencies:
- other: no reliability is given as this is a summary entry for the BUA report
- Principles of method if other than guideline:
- BUA report
- GLP compliance:
- not specified
- Executive summary:
BUA report, 1987:
Extended fish tests (14 d) gave an NOEC of 0.04 mg/l (Broecker et al., 1984).
The BUA report doesn't state NOEC values for other above cited studies.
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Original reference not available.
- GLP compliance:
- not specified
- Test organisms (species):
- Jordanella floridae
- Executive summary:
Smith, 1991:
egg hatchability: 1.43 mg/L
10d larval survival: 1.13 mg/L
lowest chronic level: 1.13 mg/L
dilution water: dechlorinated Lake Superior water 25 +/- 2 °C- Endpoint:
- fish early-life stage toxicity
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1995
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- abstract
- Remarks:
- GLP-Guideline study, only results are published, study report not available
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 204 (Fish, Prolonged Toxicity Test: 14-day Study)
- Deviations:
- not specified
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 210 (Fish, Early-Life Stage Toxicity Test)
- Deviations:
- not specified
- GLP compliance:
- yes
- Analytical monitoring:
- not specified
- Vehicle:
- not specified
- Test organisms (species):
- Oryzias latipes
- Water media type:
- freshwater
- Limit test:
- no
- Duration:
- 14 d
- Dose descriptor:
- LC50
- Effect conc.:
- 1.6 mg/L
- Remarks on result:
- other: OECD TG 204
- Duration:
- 21 d
- Dose descriptor:
- LC50
- Effect conc.:
- 1.5 mg/L
- Remarks on result:
- other: OECD TG 210
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.26 mg/L
- Remarks on result:
- other: OECD TG 210
- Executive summary:
NITE,1995:
The toxicity of 1,2,4-TCB to Oryzias latipes in fresh water was investigated according to OECD guideline 204 and 210:
14 d; LC50 = 1.6 mg/L (OECD 204)
21 d; LC50 = 1.5 mg/L (OECD 210)
21 d; NOEC = 0.26 mg/L (OECD 210)
- Endpoint:
- adult fish: sub(lethal) effects
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Study period:
- 1984
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: GLP-Guideline study. Test procedure according to national standards.
- Qualifier:
- according to guideline
- Guideline:
- other: Prüfrichtlinie I 4.5 - 97125-2/6, Umweltbundesamt, 06.05.1982
- GLP compliance:
- yes
- Analytical monitoring:
- yes
- Vehicle:
- no
- Test organisms (species):
- Danio rerio (previous name: Brachydanio rerio)
- Test type:
- flow-through
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.04 mg/L
- Nominal / measured:
- meas. (not specified)
- Duration:
- 21 d
- Dose descriptor:
- other: LC20
- Effect conc.:
- 0.18 mg/L
- Nominal / measured:
- meas. (not specified)
- Duration:
- 21 d
- Dose descriptor:
- other: LC20
- Effect conc.:
- 0.7 mg/L
- Nominal / measured:
- meas. (not specified)
- Duration:
- 21 d
- Dose descriptor:
- other: LC100
- Effect conc.:
- 1.9 mg/L
- Nominal / measured:
- meas. (not specified)
- Executive summary:
Markert, 1984:
The toxicity of 1,2,4-TBC to Danio rerio (Brachydanio rerio) was investigated in a 21 d study according to test guideline 4.5 - 97125-2/6, Federal Environmental Agency, 06.05.1982. The 21d-NOEC value was determined to be 0.04 mg/L.
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Original reference not available.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Test organisms (species):
- Pimephales promelas
- Duration:
- 8 d
- Dose descriptor:
- LC50
- Effect conc.:
- 1.8 mg/L
- Executive summary:
Hall, 1984:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 8-day study. The LC50 value was determined as 1.8 mg/L and analytically monitored by GC.
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Original reference not available.
- GLP compliance:
- no
- Test organisms (species):
- Pimephales promelas
- Duration:
- 32 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.29 mg/L
- Executive summary:
Atri, 1986:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 32-day study. The NOEC value was determined as 0.29 mg/L.
- Endpoint:
- long-term toxicity to fish, other
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 4 (not assignable)
- Rationale for reliability incl. deficiencies:
- other: Original reference not available.
- GLP compliance:
- not specified
- Analytical monitoring:
- yes
- Test organisms (species):
- Pimephales promelas
- Duration:
- 32 d
- Dose descriptor:
- other: EC0
- Effect conc.:
- 0.5 - 1 mg/L
- Executive summary:
Ahmad, 1984:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 32-day study. The LC0 value was determined as 0.5 mg/L and analytically monitored by GC.
Referenceopen allclose all
EU Risk Assessment (2003):
Long-term studies on fish are all based on measured concentrations. The reported values for NOEC are close together. They range between 0.04 mg/l (Broecker et al., 1984) and 0.50 mg/l (Carlson and Kosian, 1987). All in EU RAR cited studies are considered to be valid.
The study on Zebrafish (Brachydanio rerio) was performed according to a UBA guideline
(1982) in a dynamic system. The fish were 4 to 7 months old. The aquarium contained 36 l and the flow was 1.2 l/h. The concentrations were measured. LOEC was 0.18 mg/l (20% mortality and several behavioural abnormalities) (Broecker et al., 1984).
Suter and Rosen (1988) report the chronic MATC value to the marine fish Cyprinodon variegatus to be 0.222 mg/l. NOEC is estimated to be approximately half this value.
The lowest NOEC in long-term studies was 0.04 mg/l (mortality and behaviour, Zebra fish).
BUA report (1987):
Extended fish tests (14 d) gave an NOEC of 0.04 mg/l (Broecker et al., 1984).
The BUA report doesn't state NOEC values for other above cited studies.
- depending up on test concentration fishes showed behaviour modifications
- some of them were: swimming on water surface and bottom of tank, accelerated respiration, trembling, apathy
Description of key information
For transported isolated intermediates according to REACh, Article 18, this endpoint is not a data requirement. However, data is available for this endpoint and is thus reported under the guidance of "all available data".
Generally, following values have been reported for the long-term toxicity to fish:
NOEC: 0.04 - 0.5 mg/L
LOEC: 0.18 mg/L
The values were obtained with respect to different guidelines and exposure times.
EU Risk Assessment, 2003:
Long-term studies on fish are all based on measured concentrations. The reported values for NOEC are close together. They range between 0.04 mg/l (Broecker et al., 1984) and 0.50 mg/l (Carlson and Kosian, 1987). All in EU RAR cited studies are considered to be valid.
The study on Zebrafish (Brachydanio rerio) was performed according to a UBA guideline (1982) in a dynamic system. The fish were 4 to 7 months old. The aquarium contained 36 l and the flow was 1.2 l/h. The concentrations were measured. LOEC was 0.18 mg/l (20% mortality and several behavioural abnormalities) (Broecker et al., 1984).
Suter and Rosen (1988) report the chronic MATC value to the marine fish Cyprinodon variegatus to be 0.222 mg/l. NOEC is estimated to be approximately half this value. The lowest NOEC in long-term studies was 0.04 mg/l (mortality and behaviour, Zebra fish).
BUA report, 1987:
Extended fish tests (14 d) gave an NOEC of 0.04 mg/l (Broecker et al., 1984).
The BUA report doesn't state NOEC values for other above cited studies.
Smith, 1991:
egg hatchability: 1430 µg/l
10d larval survival: 1130 µg/l
lowest chronic level: 1130 µg/l
dilution water: dechlorinated Lake Superior water 25 +/- 2 °C
Hall, 1984:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 8-day study. The LC50 value was determined as 1.8 mg/L and analytically monitored by GC.
Atri, 1986:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 32-day study. The NOEC value was determined as 0.29 mg/L.
Ahmad, 1984:
The toxicity of 1,2,4-TCB to Pimephales promelas was investigated in an 32-day study. The LC0 value was determined as 0.5 mg/L and analytically monitored by GC.
NITE,1995:
The toxicity of 1,2,4-TCB to Oryzias latipes in fresh water was investigated according to OECD guideline 204 and 210:
14 d; LC50 = 1.6 mg/L (OECD 204)
21 d; LC50 = 1.5 mg/L (OECD 210)
21 d; NOEC = 0.26 mg/L (OECD 210)
Markert, 1984:
The toxicity of 1,2,4-TBC to Danio rerio (Brachydanio rerio) was investigated in a 21 d study according to test guideline 4.5 - 97125-2/6, Federal Environmental Agency, 06.05.1982. The 21d-NOEC value was determined to be 0.04 mg/L.
Key value for chemical safety assessment
Additional information
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
