Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 204-428-0 | CAS number: 120-82-1
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Toxicity to microorganisms
Administrative data
- Endpoint:
- activated sludge respiration inhibition testing
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: Acceptable, well documented publication which meets basic scientific principles.
Data source
Reference
- Reference Type:
- publication
- Title:
- Estimating Toxicity of Organic Chemicals to Activated-Sludge Microorganisms.
- Author:
- Sun B et al.
- Year:
- 1 994
- Bibliographic source:
- J. Environ. Eng. (N.Y.) 120 (6)
Materials and methods
- Principles of method if other than guideline:
- => testing of biodegradation of 1,2,4-TCB by municipal sewage (activated sludge) in closed vessels
=> inhibition effects on microorganisms were measured by reductions in oxygen uptake (BOD)
Activated-Sludge Test Cultures:
The activated sludge test cultures were obtained daily from the Las Cruces Wastewater Treatment Plant, which receives mainly municipal sewage. The reactor (test vessels) received 10 ml of activated sludge each. The test reactors were topped with tap water to reach the final volume of 60 ml, and the control reactors were topped up to 62 ml. All the reactors were then capped with potassium hydroxide pellets in holders attached to the caps. The tests were conducted at the endogenous phase, without any additional growth substrate.
Respirometric Test Procedure:
The tests were run on a 12-reactor, computer-interfaced Comput-OX Respirometer (N-CON Corporation, N.Y.). Except of the control, each reactor was administered with different volumes of the toxicant. The toxicant dose was prepared by dissolving the toxicant in 2 ml of acetone and injecting into the liquid phase in each reactor. The capped reactors were placed in the respirometer water bath, maintained at 25°C with continued supply of oxygen. The data acquisition system was then initiated to monitor and record the oxygen uptake of each reactor; for the next 12 hours.
The inhibition effects were measured in IC50 terms. The percent inhibition at different concentrations of the toxicant was taken as reductions in oxygen uptake rates of spiked reactors compared to that of the control reactor. These percent inhibition values were then plotted against the respective concentrations, and from linear regression analysis of these plots in the range of 2080% inhibition, the concentration causing 50% inhibition, IC50 was determined. - GLP compliance:
- not specified
Test material
- Reference substance name:
- 1,2,4-trichlorobenzene
- EC Number:
- 204-428-0
- EC Name:
- 1,2,4-trichlorobenzene
- Cas Number:
- 120-82-1
- Molecular formula:
- C6H3Cl3
- IUPAC Name:
- 1,2,4-trichlorobenzene
- Details on test material:
- - Name of test material (as cited in study report): 1,2,4-Trichlorobenzene
- Analytical purity: no data
- Impurities (identity and concentrations): no data
Constituent 1
Sampling and analysis
- Analytical monitoring:
- not specified
- Details on sampling:
- The tests were run on a 12-reactor, computer-interfaced Comput-OX Respirometer (N-CON Corporation, N.Y.). Details of this system have been presented elsewhere (Cadena et al., 1988). The data acquisition system was initiated to monitor and record the oxygen uptake of each reactor; for the next 12 hours.
Test solutions
- Vehicle:
- not specified
Test organisms
- Test organisms (species):
- activated sludge of a predominantly domestic sewage
- Details on inoculum:
- The activated sludge test cultures were obtained daily from the Las Cruces Wastewater Treatment Plant, which receives mainly municipal sewage.
The tests were conducted at the endogenous phase, without any additional growth substrate.
Study design
- Test type:
- static
- Water media type:
- freshwater
- Total exposure duration:
- 12 h
Test conditions
- Test temperature:
- 25°C
[The capped reactors were placed in the respirometer water bath, maintained at 25°C with continued supply of oxygen.] - Details on test conditions:
- Test System:
- Type: closed
- Aeration: continued supply of oxygen
All the reactors were then capped with potassium hydroxide pellets in holders attached to the caps.
The reactor (test vessels) received 10 ml of activated sludge each. The test reactors were topped with tap water to reach the final volume of 60 ml,
and the control reactors were topped up to 62 ml. - Reference substance (positive control):
- no
Results and discussion
Effect concentrations
- Duration:
- 12 h
- Dose descriptor:
- IC50
- Effect conc.:
- 35 mg/L
- Basis for effect:
- inhibition of total respiration
- Remarks:
- respiration rate
Applicant's summary and conclusion
- Validity criteria fulfilled:
- not specified
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.