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Toxicological information

Dermal absorption

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Administrative data

Endpoint:
dermal absorption in vivo
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable, well documented publication

Data source

Reference
Reference Type:
secondary source
Title:
Whole-body autoradiography after systemic and topical administration of methyl acrylate in the guinea-pig.
Author:
Seutter E and Rijntes NVM
Year:
1981
Bibliographic source:
Arch. Dermatol. Res. 270: 273-284

Materials and methods

Test guideline
Qualifier:
no guideline followed
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl acrylate
EC Number:
202-500-6
EC Name:
Methyl acrylate
Cas Number:
96-33-3
Molecular formula:
C4H6O2
IUPAC Name:
methyl acrylate
Details on test material:
- Name of test material (as cited in study report): Methyl acrylate
- Analytical purity: 99 % (GC)
- Supplier: Merck, Darmstadt

- Radiochemical purity (if radiolabelling): no data
- Specific activity (if radiolabelling): 0.76 mCi/mmol
- Locations of the label (if radiolabelling): Methyl (2,3-14C)-acrylate
- Supplier: Radiochemical Centre, Amersham, England
Radiolabelling:
yes

Test animals

Species:
guinea pig
Strain:
not specified
Sex:
male
Details on test animals or test system and environmental conditions:
- Weight at study initiation: 420 g

Administration / exposure

Type of coverage:
occlusive
Vehicle:
unchanged (no vehicle)
Doses:
0.53 mmol/kg bw (corresponding to approx. 46 mg/kg bw)
No. of animals per group:
2 animals received a dose of 0.53 mmol/kg bw of the unlabeled acrylate (corresponding to approx. 46 mg/kg bw), 6 animals received the same dose of the labeled acrylate.
Control animals:
no
Details on study design:
APPLICATION OF DOSE:
For administration on the skin a polystyrene cup of 16 mm inner diameter was glued to the shaved skin of the back with Nobecutane (Astra). After a few minutes of slight pressure the rim of the cup was connected tightly with the skin. The acrylate was injected into the cup, which was immediately closed with a lid and fastened in this position with elastic adhesive bandage of 3" width, wound around the animal in such a way as not to hinder respiration.


SITE PROTECTION / USE OF RESTRAINERS FOR PREVENTING INGESTION: yes

Results and discussion

Signs and symptoms of toxicity:
not specified
Dermal irritation:
yes

Any other information on results incl. tables

After administration in a closed cup on skin (46 mg/kg bw), a slow penetration in dermis occurred and low levels were found in the kidneys and bladder within 4 hours. After penetration of the dermis, which occurred between 6 and 40 hours, a gradual increase of radioactivity occurred in the heart, liver, lungs, tear glands, and other subcutaneous tissues throughout the body. The detoxification was screened by estimating the urinary thioether content and respiratory carbon monoxide. This showed that in addition to oxidation to CO2, the binding to SH-groups was the principal means of metabolism.

Applicant's summary and conclusion