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EC number: 202-500-6
CAS number: 96-33-3
• In the control the
mortality of parent animals up to the end of the test was 0 %.
• In the control the
mean number of living young per parent animal that survived the test was
• No ephippia were
produced in the controls.
In conclusion the test
was considered to be valid.
ANALYTICAL CONCENTRATION CONTROL
At the start of
exposure, samples for analysis were collected from the stock solution
only because the analytical laboratory reported an LOQ of 4.648 mg/L.
For each measurement interval, the stock solution was kept under test
conditions. During course of the experiment, the analytical lab lowered
the LOQ to 0.513 mg/L. Based on this information, the three highest test
concentrations were analyzed over 3 daily renewal intervals starting on
day 16 of the study. The final calculated LOQ reported by the analytical
laboratory was 0.104 mg/L.
measured concentrations of the stock solution were lower than the
expected nominal concentrations, most likely due to some volatilization
of the test substance during solution preparation. However, all measured
concentrations were within ± 20% of the measured mean initial
concentration indicating that the test substance was stable under test
conditions at the stock concentration.
determined concentrations of the test substance in the test solutions
deviated by more than 20% from the nominal or mean initial measured
concentrations. Therefore the time-weighted mean measured concentrations
were calculated and will be used to evaluate the biological results.
measured concentrations of n-Butyl acrylate in the stock solutions:
Analytically measured concentration
a: % of mean initial
b: % of nominal
measured concentrations of n-Butyl acrylate in the test solutions:
24 h old
n.d.: not determined
Growth Summary (mean per surviving replicates after 21 days):
Mean living young
Mean length [mm]
a: Coefficient of
variation for control fecundity
b: Effect relative to
control (only calculated for statistically significant effects).
No data is available on long-term effects to Methyl Acrylate.
The category document provided in IUCLID section 13 provides further information on the category and the read-across approach. An overview of the long-term effects on inverterbrates on acrylate esters is provided below.
In the structurally related n-butyl acrylate, ethyl acrylate and 2-Ethylhexyl acrylate, NOECs of 0.136 , 0.19 mg/L and 0.85 mg/L respectively were determined for long-term toxicity towards Daphnia magna.
Data from the structurally related n-butyl acrylate:NOEC (reproduction) = 0.136 mg/L (Daphnia magna, OECD 211, semi-static)
Data from the structurally related ethyl acrylate: NOEC (reproduction) = 0.19 mg/L (Daphnia magna, EPA OTS 797.1330, flow-through)
Data from structurally related 2-Ethylhexyl acrylate:
EC10 (reproduction)= 0.85 mg/L TWM (Daphnia magna, OECD 211, semi-static)
There are no data on long-term toxicity towards invertebrates for methyl acrylate. Long-term 21 -day chronic toxicity studies are available for the structurally related ethyl acrylate, 2-ethylhexyl acrylate and n-butyl acrylate (BAMM 1997, Charles River 2020, BASF SE 2009).
A 21-day Daphnia magna Reproduction Test sponsored by The Acrylate REACh Task Force was performed with n-Butyl acrylate under semi-static conditions in accordance with OECD TG 211 and GLP regulations (BASF SE, 2009). Nominal test concentrations were 0, 0.1, 0.22, 0.46, 1.0, and 2.2 mg/L. Since the mean values of n-Butyl Acrylate in M4-medium were found to be in the range of 9.3 – 80.0 % of the nominal concentrations, the effect concentrations were based on the time-weighted mean analytically detected concentrations. The most sensitive effect values were concerning reproduction: NOEC (reproduction) = 0.136 mg/L; LOEC (reproduction) = 0.457 mg/L. Survival of the parent or young daphnia was not affected, but the growth of the parent animals was impacted: NOEC (body length) = 0.457 mg/L; LOEC (body length) = 1.226 mg/L.
In addition, a 21-day chronic study with Ethyl acrylate in Daphnia magna was conducted under flow-through conditions according to EPA OTS 797.1330 (BAMM, 1997). Nominal exposure concentrations ranged from 0.38 to 6.0 mg/L. Mean measured test concentrations ranged from 0.19 to 3.2 mg/L. Effect concentrations based on measured values were as follows: EC50 was 0.5 mg/L; NOEC (reproduction) = 0.19 mg/L; LOEC (reproduction) = 0.45 mg/L and MATC (reproduction) = 0.29 mg/L.
The effects of the long-term exposure of Daphnia magna to 2-ethylhexyl acrylate according to OECD Guideline 211 have been studied (Charles River, 2020).
The test was performed in a closed system with a semi-static renewal. Five test concentrations ranging from 5.6, 10, 18, 32 and 56% of a saturated solution prepared at a loading rate of 50 mg/L (TMW concentrations: 0.21, 0.44, 0.85, 1.4, 2.8 mg/L respectively) were employed. The effects values were related to TWM concentrations and are given as follows: 21d-EC10 (growth)= 0.85mg/L; 21d-EC10 (reproduction)= 0.91mg/L ; 21d-EC50 (mortality)= 1.6 mg/L.
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