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Ecotoxicological information

Toxicity to microorganisms

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Description of key information

The inhibition of the degradation activity of activated sludge is not anticipated when Methyl acrylate is introduced into biological treatment plants in appropriately low concentrations.
EC10 (3d) > 100 mg/L (BOD Test, domestic activated sludge)

Key value for chemical safety assessment

Additional information

Concerning acute toxicity of methyl acrylate towards aerobic microorganisms, a study conducted by Roy F. Weston Inc. for BAMM is available (BAMM, 1996). The test procedure was based on ASTM's "Microbiological Inhibition Testing Procedure," and was a modification of the standard five day biochemical oxygen demand (BOD) analysis. The test measured the threshold inhibition concentration of the test substance to a mixed microbial inoculum by measuring the oxidation change over various concentrations of the test substance and comparing this to the oxidation change of an easily degradable substance such as D-glucose. The threshold inhibition concentration was defined as the lowest concentration of the test substance which produces a reduction in biochemical oxidation. Activated sludge originated from the Downingtown Regional Water Pollution Control Center in Downingtown, Pennsylvania. This facility receives predominately domestic waste. Effect values after 72 hrs exposure were: EC10 > 100 mg/L.

In a static acute toxicity test with Pseudomonas putida according to the German Standard DIN38412, part 8 the growth inhibition was determined photometrically (BASF AG 1988). After 17 hours incubation the EC50 based on nominal concentrations was 260 mg/L; the EC10 was 131 mg/L.

Taking all these test results into consideration, inhibition of the degradation activity of activated sludge in a WWTP is not anticipated when the substance is introduced in appropriately low concentrations in WWTPs.