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Toxicological information

Genetic toxicity: in vivo

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Administrative data

Endpoint:
in vivo mammalian somatic cell study: cytogenicity / erythrocyte micronucleus
Remarks:
Type of genotoxicity: chromosome aberration
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Well documented study with acceptable restrictions (group size not specified)

Data source

Reference
Reference Type:
publication
Title:
Cytogenetic Effects of Gaseous and Volatile Chemicals on Mammalian Cells in vitro and in vivo II. Micronucleus tests in mice.
Author:
Sofuni T, Hayashi M, Matsouka A, Sawada M, Hatanaka M, Ishidate M Jr.
Year:
1984
Bibliographic source:
Bull National Institute of Hygiene Sciences 102:84-90, 1984 (in Japanese; Translation available)

Materials and methods

Principles of method if other than guideline:
Groups of 2 male ddY mice were exposed to methyl acrylate vapours at concentrations of 1300 and 2100 ppm (corresponding to 4.64 and 7.50 mg/L) for three hours. Bone marrow cells from the right femurs were collected at 18, 24, 30, 48 or 72 hours after exposure.
GLP compliance:
not specified
Type of assay:
micronucleus assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl acrylate
EC Number:
202-500-6
EC Name:
Methyl acrylate
Cas Number:
96-33-3
Molecular formula:
C4H6O2
IUPAC Name:
methyl acrylate
Details on test material:
- Supplier: Obtained from Wako Pure Chemical Industries, Ltd.
- Analytical purity: unknown

Test animals

Species:
mouse
Strain:
other: ddY mouse
Sex:
male
Details on test animals or test system and environmental conditions:
TEST ANIMALS
- Source: Shizuoka Jiken . Dobutsu Kyodo Kumiai (Shizuoka Experimental Animals Co-operative)
- Age at study initiation: 7 weeks old
- Acclimation period: 1 week

Administration / exposure

Route of administration:
inhalation: vapour
Details on exposure:
TYPE OF INHALATION EXPOSURE: whole body


GENERATION OF TEST ATMOSPHERE / CHAMBER DESCRIPTION
- Exposure apparatus: The exposure chambers had internal diameters of 185 mm, heights of 160 mm and internal volumes of 5.85 L.
- Method of holding animals in test chamber: 4-6 mice in cylindrical stainless steel cages
- Source and rate of air: Test atmospheres were obtained from volatile chemical substances using an automatic injector and a hose type gas generator. Air which had been purified by passing it through a filter was fed into the hose at a pressure of 1 kg/cm2. In order to maintain a set pressure, a set volume of air was vented. A capillary tube was connected between the automatic injector and the hose and the test chemical was injected from this
capillary tube at a set flow rate. The sample injected into the hose was heated there to its boiling point, or higher, where upon it vapourized and was mixed with air as it flowed through the hose, so as to produce a set concentration of vapour. The flow rate inside the chamber was regulated to be 3000 mL/min.
- Temperature, humidity, pressure in air chamber: no data
- Air flow rate: 3000 mL/min
- Air change rate: 30 times per hour


TEST ATMOSPHERE
- Brief description of analytical method used: GC/FID (Shimadzu GC-9A gas chromatograph)
Duration of treatment / exposure:
3 hours
Frequency of treatment:
once
Doses / concentrations
Remarks:
Doses / Concentrations:
1300, 2100 ppm (4.64 mg/L und 7.50 mg/L)
Basis:
analytical conc.
by GC determination
No. of animals per sex per dose:
2
Control animals:
yes, historical
Positive control(s):
no

Examinations

Tissues and cell types examined:
Bone marrow cells were collected from the right femurs
Details of tissue and slide preparation:
CRITERIA FOR DOSE SELECTION:
Doses were selected based on prescreening which showed that at 1700 ppm there was a slight decrease in PCE ratio and that some animals exposed to 2100 ppm for 3 hours survived only 24 - 48 hours. Therefore it was not advisable to raise the concentration any higher.


Results and discussion

Test results
Sex:
male
Genotoxicity:
negative
Toxicity:
yes
Remarks:
At 2100 ppm 1/2 mice died after 24 and 48 hr, respectively.
Vehicle controls validity:
not applicable
Negative controls validity:
not specified
Positive controls validity:
not specified
Additional information on results:
There was a slight increase in the frequency of MNPCE's in the specimens prepared 30 hours after exposure to 1300 ppm of methyl acrylate, but the frequencies for the 2100 ppm groups were not significantly different from that of the control group in this laboratory (0 .22) and no clear increase in the frequency of MNPCE's was observed overall.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information): negative