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EC number: 202-500-6 | CAS number: 96-33-3
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
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- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
- Biodegradation
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- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
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- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Acute Toxicity: inhalation
Administrative data
- Endpoint:
- acute toxicity: inhalation
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP guideline study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 012
- Report date:
- 2012
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 403 (Acute Inhalation Toxicity)
- GLP compliance:
- yes (incl. QA statement)
- Test type:
- standard acute method
Test material
- Reference substance name:
- Methyl acrylate
- EC Number:
- 202-500-6
- EC Name:
- Methyl acrylate
- Cas Number:
- 96-33-3
- Molecular formula:
- C4H6O2
- IUPAC Name:
- methyl prop-2-enoate
- Details on test material:
- - Name of test material (as cited in study report): Methylacrylate
- Physical state: liquid /colorless, clear
- Analytical purity: 99.95 %
- Lot/batch No.: 011063eda0
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male/female
- Details on test animals or test system and environmental conditions:
- TEST ANIMALS
- Source: Haraln Laboratories B.V., Niederlande
- Age at study initiation: Young adult animals (male: approx. 8 weeks; female approx. 10 weeks)
- Weight at study initiation: animals of vomparable weight (+/- 20% of the mean weight)
- Housing: single housing
- Diet (e.g. ad libitum): ad libitum
- Water (e.g. ad libitum): ad libitum
- Acclimation period:for at least 5 days before exposure
ENVIRONMENTAL CONDITIONS
- Temperature (°C): 20 -24 °C
- Humidity (%): 30 - 70 %
- Air changes (per hr): 12/12
Administration / exposure
- Route of administration:
- inhalation: vapour
- Type of inhalation exposure:
- nose/head only
- Vehicle:
- air
- Details on inhalation exposure:
- Head-nose inhalation system INA 60 (glass-steel construction, BASF SE, volume V ≈ 90 L): the animals were restrained in glass tubes and their snouts projected into the inhalation system.
The homogenous distribution of test substance atmosphere in this inhalation system has been verified with model vapors.
GENERATION OF TEST ATMOSPHERE
Conditioned air:
Central air conditioning system provides cold air of about 15°C. This cold air will pass through an activated charcoal filter, be adjusted to room temperature of 20 to 24°C and pass through a second particle filter (H13 (HEPA) Camfil Farr, Germany). The so generated conditioned air is used to generate inhalation atmospheres.
Compressed air:
Compressed air is produced by an oil-free compressor (HT 6, Josef Mehrer GmbH & Co KG, Germany). For this purpose, air is filtered by an inlet air strainer and introduced into the compressor. After passed through an second ultra filter (SMF 5/3, 108 mm, Donalson), the compressed air (15 bar) is stored in a storage of 1500 or 5000 L. The compressed air is conducted to the labs via pipes, where the pressure is reduced to 6 bar. In the lab, thecompressed air can be taken as required.
Exhaust air:
The exhaust air is filtered and conducted into the exhaust air of the building. The exposure system was located inside an exhaust cabin in an air-conditioned laboratory. During each exposure, the following parameters were recorded four times at about 1-hour intervals:
Supply air flow (conditioned air): 3.0 m³/h (from a central air-conditioning system) The flow was adjusted and continuously measured with a flowmeter (Yokogawa).
Exhaust air flow: 2.6 m³/h The flow was adjusted and continuously measured with a flowmeter (Yokogawa).
The lower amount of exhaust air, which was adjusted by means of a separate exhaust air system, achieved a positive pressure inside the exposure system. This ensured that the mixture of test substance and air was not diluted with laboratory air in the breathing zones of the animals.
An air change of about 33 times per hour can be calculated by dividing the supply air flows by the volume of the inhalation system.
The animals were exposed to the inhalation atmosphere for 4 hours plus equilibration time of the inhalation systems (t99 about 8 min).
No surveillance of the oxygen content in the inhalation system was performed. The air change was judged to be sufficient to prevent oxygen depletion by the breathing of the animals, and the concentration of the test substance used could not have a substantial influence on oxygen partial pressure.
Temperature: The temperature in the inhalation system was measured continuously with a digital thermometer.
Humidity: The humidity in the inhalation system was measured with a dielectric probe (Testo). - Duration of exposure:
- 4 h
- Concentrations:
- 10.832 mg/l
- No. of animals per sex per dose:
- 5
- Control animals:
- no
- Details on study design:
- - Duration of observation period following administration: 14 days
- Frequency of observations and weighing: The body weight on the animals was checked before the beginning of the study and at least on days 1,3 and 7 and before the sacrifice of the animals at the end of the observation period. Clinical findings were recorded several times during exposure and at least once on each workday in the observation period. A check for dead animals were made daily.
- Necropsy of survivors performed: yes
Results and discussion
Effect levels
- Sex:
- male/female
- Dose descriptor:
- LC50
- Effect level:
- < 10.832 mg/L air (analytical)
- Based on:
- test mat.
- Exp. duration:
- 4 h
- Mortality:
- 5 males and 2 females died at the test concentration
- Clinical signs:
- other: depressed respiration, abdominal respiration, gasping, respiration sounds, red encrusted eye and nose, salivation, skin pale, piloerection, hyperexcitability, tremor and poor general state. Findings were observed from hour 2 of exposure through to the stu
- Body weight:
- No body weight data present in the male animals because all males died. The mean body weights of the surviving female animals decreased during the first post exposure observations day and increased from study day 3 onward.
- Gross pathology:
- No gross pathological abnormalities were detected during necropsy in the female animal that was found dead on study day 0 after exposure. During necropsy of the male animals that was found dead on study day 1 few dark-red discolorations in the lung with partly sunken surface and severe
dilation with a gaseous content was noted. Another male animal which also died on study day 1, showed only severe dilation with a gaseous content. During necropsy of the three males that was found dead on study day 2, abnormal gaseous content of the stomach and intestine was noted. The one female animal that was found dead on study day 2 showed dark-red discoloration in the lung and abnormal gaseous content of the stomach and intestine. No gross pathological abnormalities were detected during the necropsy in the surviving three female animals at the termination of the post exposure period.
Any other information on results incl. tables
Results of analytical measurements:
Sample No. Analytical concentration (mg/L)
101 9.961
102 10.990
103 11.310
104 11.067
Mean 10.8320
Last fritted glass flask (sample 105) 0.000
Mean + Last fritted glass flask 10.8320
Mean (rounded) 10.832
Standard deviation of the mean 0.596
Nominal concentration 10.4
Applicant's summary and conclusion
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
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