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Toxicological information

Genetic toxicity: in vitro

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Administrative data

Endpoint:
in vitro gene mutation study in bacteria
Remarks:
Type of genotoxicity: gene mutation
Type of information:
experimental study
Adequacy of study:
supporting study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: Acceptable publication which meets basic scientific principles (results only in tabular form)

Data source

Referenceopen allclose all

Reference Type:
publication
Title:
Assay of 855 Test Chemnicals in Ten Tester Strains Using a New Modification of the Ames Test for Bacterial Mutagens.
Author:
McMahon RE et al.
Year:
1979
Bibliographic source:
Cancer Res. 39: 682-693
Reference Type:
secondary source
Title:
Assay of 855 Test Chemnicals in Ten Tester Strains Using a New Modification of the Ames Test for Bacterial Mutagens.
Author:
McMahon RE et al.
Year:
1979
Bibliographic source:
Cancer Res. 39: 682-693, 1979; cited in: Review Methylacrylat, Dr. Zeller, (1986)

Materials and methods

Principles of method if other than guideline:
Plate gradient assay
GLP compliance:
not specified
Type of assay:
bacterial reverse mutation assay

Test material

Constituent 1
Chemical structure
Reference substance name:
Methyl acrylate
EC Number:
202-500-6
EC Name:
Methyl acrylate
Cas Number:
96-33-3
Molecular formula:
C4H6O2
IUPAC Name:
methyl acrylate
Details on test material:
Methyl acrylate, no further data

Method

Target gene:
Histidine auxotrophy (S. typhimurium), tryptophn auxotrophy (E. coli)
Species / strain
Species / strain / cell type:
other: Salmonella typhimurium G 46, TA 1535, TA 100, C 3076, TA 1537, D 3052 TA 1538,TA 98, E. coli WP2, E. coli WP2uvrA-
Metabolic activation:
with and without
Metabolic activation system:
liver S-9 mix from Aroclor induced rats
Test concentrations with justification for top dose:
0.1 to 1000 ug/ml
Controls
Untreated negative controls:
yes
Negative solvent / vehicle controls:
no
True negative controls:
no
Positive controls:
yes
Positive control substance:
other: Without S-9: streptozotocin, with S-9: 2-acetylaminofluorene
Details on test system and experimental conditions:
Ames test

Results and discussion

Test results
Species / strain:
other: Salmonella typhimurium G 46, TA 1535, TA 100, C 3076, TA 1537, D 3052 TA 1538,TA 98, E. coli WP2, E. coli WP2uvrA-
Metabolic activation:
with and without
Genotoxicity:
negative
Cytotoxicity / choice of top concentrations:
no cytotoxicity
Vehicle controls validity:
not applicable
Untreated negative controls validity:
valid
Positive controls validity:
valid
Remarks on result:
other: all strains/cell types tested
Remarks:
Migrated from field 'Test system'.

Applicant's summary and conclusion

Conclusions:
Interpretation of results (migrated information):
negative