Pyrocatechol

Immunotoxicity

Administrative data

Endpoint:

immunotoxicity, other

Remarks:

acute

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

Control results are not reported. No conclusion on inhibitory effects of catechol on IgM production of HB4C5 cells can be drawn as the test concentrations should be considered as cytotoxic. No data about the purity of substance.

Data source

Reference

Reference Type:

publication

Title:

Effects of phenyl compounds on prolifeartion and IgM production of Human-human hybridoma HB4C5 cells cultured in serum-free medium

Author:

Maeda M., Yamada K., Ikeda I., Nakajima H., Tajima M., Murakami H.

Year:

1990

Bibliographic source:

Agricultural and Biological Chemistry, 54 (4), 1093-1096.

Materials and methods

Principles of method if other than guideline:

In vitro effects on IgM production of human hybridoma cells

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Pyrocatechol

EC Number:

204-427-5

EC Name:

Pyrocatechol

Cas Number:

120-80-9

Molecular formula:

C6H6O2

IUPAC Name:

pyrocatechol

Details on test material:

Purchased from Sigma, purity unknown.

Test animals

Species:

other: human cells

Strain:

other: hybridoma cells

Administration / exposure

Route of administration:

other: in vitro

Vehicle:

other: phosphate buffered saline (PBS)

Duration of treatment / exposure:

Exposure period: 2 day(s)

Doses / concentrations

Remarks:

Doses / Concentrations:
1.1 - 11 - 110 µg/mL|(10 - 100 - 1000 µM)
Basis:

Control animals:

yes

Details on study design:

- Test system:
Human-human hybridoma HB4C5 cells cultured in a serum-free medium were used. They produce IgM specific to human lung adenocarcinoma cells and tissues.

- Test protocol:
The cells were inoculated at a cell density of 5 x 10E4 cells/mL into 24-well multiplates, and cultured in ERDF medium supplemented with 10 µg/mL insulin, 35 µg/mL transferrin, 10 µM ethanolamine, and 2.5 nM selenium containing catechol at 37°C for 2 days.
3 catechol concentrations were tested, final concentrations were 1.1, 11, 110 µg/mL. The IgM content in the culture supernatant was measured by
the ELISA method.
IgM productivity of the cells was calculated by dividing the IgM content by the cell number on the second day.
As a standard, human serum IgM was used.

Results and discussion

Results of examinations

Details on results:

The relative growth rate is expressed as the ratio of the cell number on the 2nd day to the first cell number.
The productivity is expressed as the ratio of the sample IgM production to the control on the second day.
At 1.1 µg/mL catechol, the relative growth rate was 0.91 and the productivity was 0.63.

At 11 µg/mL catechol, the relative growth rate was 0.61 and the productivity was 0.32.

At 110 µg/mL catechol, the relative growth rate was 0.56 and the productivity was 0.30.

Applicant's summary and conclusion

Conclusions:

Ambiguous.