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Toxicological information

Immunotoxicity

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Administrative data

Endpoint:
immunotoxicity
Remarks:
acute
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: Control results are not reported. No conclusion on inhibitory effects of catechol on IgM production of HB4C5 cells can be drawn as the test concentrations should be considered as cytotoxic. No data about the purity of substance.

Data source

Reference
Reference Type:
publication
Title:
Effects of phenyl compounds on prolifeartion and IgM production of Human-human hybridoma HB4C5 cells cultured in serum-free medium
Author:
Maeda M., Yamada K., Ikeda I., Nakajima H., Tajima M., Murakami H.
Year:
1990
Bibliographic source:
Agricultural and Biological Chemistry, 54 (4), 1093-1096.

Materials and methods

Principles of method if other than guideline:
In vitro effects on IgM production of human hybridoma cells
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyrocatechol
EC Number:
204-427-5
EC Name:
Pyrocatechol
Cas Number:
120-80-9
Molecular formula:
C6H6O2
IUPAC Name:
pyrocatechol
Details on test material:
Purchased from Sigma, purity unknown.

Test animals

Species:
other: human cells
Strain:
other: hybridoma cells

Administration / exposure

Route of administration:
other: in vitro
Vehicle:
other: phosphate buffered saline (PBS)
Duration of treatment / exposure:
Exposure period: 2 day(s)
Doses / concentrations
Remarks:
Doses / Concentrations:
1.1 - 11 - 110 µg/mL|(10 - 100 - 1000 µM)
Basis:

Control animals:
yes
Details on study design:
- Test system:
Human-human hybridoma HB4C5 cells cultured in a serum-free medium were used. They produce IgM specific to human lung adenocarcinoma cells and tissues.

- Test protocol:
The cells were inoculated at a cell density of 5 x 10E4 cells/mL into 24-well multiplates, and cultured in ERDF medium supplemented with 10 µg/mL insulin, 35 µg/mL transferrin, 10 µM ethanolamine, and 2.5 nM selenium containing catechol at 37°C for 2 days.
3 catechol concentrations were tested, final concentrations were 1.1, 11, 110 µg/mL. The IgM content in the culture supernatant was measured by
the ELISA method.
IgM productivity of the cells was calculated by dividing the IgM content by the cell number on the second day.
As a standard, human serum IgM was used.

Results and discussion

Results of examinations

Details on results:
The relative growth rate is expressed as the ratio of the cell number on the 2nd day to the first cell number.
The productivity is expressed as the ratio of the sample IgM production to the control on the second day.
At 1.1 µg/mL catechol, the relative growth rate was 0.91 and the productivity was 0.63.

At 11 µg/mL catechol, the relative growth rate was 0.61 and the productivity was 0.32.

At 110 µg/mL catechol, the relative growth rate was 0.56 and the productivity was 0.30.

Applicant's summary and conclusion

Conclusions:
Ambiguous.