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EC number: 204-427-5 | CAS number: 120-80-9
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Exposure related observations in humans: other data
Administrative data
- Endpoint:
- exposure-related observations in humans: other data
- Type of information:
- experimental study
- Adequacy of study:
- other information
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Remarks:
- This test was not performed according to standardised guidelines, but is well described.
Data source
Reference
- Reference Type:
- publication
- Title:
- Wine antioxidant polyphenols inhibit the proliferation of human prostate cancer cell lines
- Author:
- Kampa M., Hatzoglou A., Notas G., Damianaki A., Bakogeorgou|E., Gemetzi C., Kouroumalis E., Martin P.-M., Castanas E.
- Year:
- 2 000
- Bibliographic source:
- Nutrition and Cancer, 37(2) 223-233.
Materials and methods
- Type of study / information:
- The effect of catechol on the growth of 3 prostate cancer cell lines (LNCaP, PC3, and DU145) was investigated.
- Endpoint addressed:
- carcinogenicity
- Principles of method if other than guideline:
- The effect of catechol on 3 prostate cancer cell lines proliferation
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Pyrocatechol
- EC Number:
- 204-427-5
- EC Name:
- Pyrocatechol
- Cas Number:
- 120-80-9
- Molecular formula:
- C6H6O2
- IUPAC Name:
- pyrocatechol
- Details on test material:
- Concentrated desalcoholised wine was prepared by vacuum distillation followed by evaporation. Wine polyphenolic extract was produced by resin adsorption of total polyphenols followed by ethanolic elution and evaporation.
(+)-catechol was prepared from the total polyphenolic extract by semipreparative high pressure liquid chromatography with use of RP18 column and assayed by ultraviolet visible spectroscopy
- Purity > 99 % (NMR at 500 MHz) - Diluted in Phosphate buffer saline (PBS).
Constituent 1
Method
- Ethical approval:
- not specified
- Exposure assessment:
- measured
- Details on exposure:
- * Cell viability and growth assay:
Cells were cultured for a total of 5 days in presence of catechol. Cell growth and viability were measured by the tetrazolium salt assay.
All experiments were performed at least 3 times in triplicates.
* H2O2 treatment:
Cells were seeded in 24-well plates at an initial density of 1.5 x 10E5 cells/well. After 24 h, medium was replaced, FBS was omitted, and catechol was introduced (10E-8 M). 24 h later, medium containing different concentrations of H2O2 (0.05-5.0 mM) was provided. After 3 h at 37 °C cells were washed with PBS and their viability was determined.
* Reactive Oxygen species generation was determined by flow cytometry.
* Androgen binding assay: LNCaP cells (1.5 x 10E5) were seeded in 24-well plates. After 24 h, binding was performed on whole cells in serum-free PBS. 435 fmol of [3H]testosterone were introduced in 50 µl PBS, together with non labelled testosterone and catechol (10E-12 to 10E-6 M).
After overnight exposure, the bound radioactivity was counted in scintillation counter. Binding was repeated at least 3 times in duplicates.
* Determination of NO production and the determination of inducible NO synthase activity were also performed.
Results and discussion
- Results:
- * Cell proliferation:
Catechol was found to significantly inhibit the proliferation of PC3 cells. LNCaP cells were less sensitive to this effect. In DU145 cells, catechol produced only partial inhibition.
* Catechol acts preferentially on the two cell lines possessing non-functional (PC3) or functional (LNCaP) androgen receptor. Results of competition experiments indicate that catechol is a very weak competitor of androgen binding.
* Preincubation with catechol resulted in an increased resistance of DU145 and PC3 cells to H2O2 toxicity. In LNCaP cells, this effect was observed only at H2O2 concentrations < 1 mM.
* Production of ROS: In PC3 cells, a significant decrease of ROS production was observed. At long incubation time (> 40 min), this effect was also observed in the DU145 cell line.
LNCaP cells did not show any modification of ROS production after catechol preincubation.
* Production of NO: Catechol decreases the production/secretion of NO.
Applicant's summary and conclusion
- Conclusions:
- Negative.
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