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Please be aware that this old REACH registration data factsheet is no longer maintained; it remains frozen as of 19th May 2023.

The new ECHA CHEM database has been released by ECHA, and it now contains all REACH registration data. There are more details on the transition of ECHA's published data to ECHA CHEM here.

Diss Factsheets

Toxicological information

Exposure related observations in humans: other data

Administrative data

exposure-related observations in humans: other data
Type of information:
experimental study
Adequacy of study:
other information
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
This test was not performed according to standardised guidelines, but is well described.

Data source

Reference Type:
Wine antioxidant polyphenols inhibit the proliferation of human prostate cancer cell lines
Kampa M., Hatzoglou A., Notas G., Damianaki A., Bakogeorgou|E., Gemetzi C., Kouroumalis E., Martin P.-M., Castanas E.
Bibliographic source:
Nutrition and Cancer, 37(2) 223-233.

Materials and methods

Type of study / information:
The effect of catechol on the growth of 3 prostate cancer cell lines (LNCaP, PC3, and DU145) was investigated.
Endpoint addressed:
Principles of method if other than guideline:
The effect of catechol on 3 prostate cancer cell lines proliferation
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
EC Number:
EC Name:
Cas Number:
Molecular formula:
Details on test material:
Concentrated desalcoholised wine was prepared by vacuum distillation followed by evaporation. Wine polyphenolic extract was produced by resin adsorption of total polyphenols followed by ethanolic elution and evaporation.
(+)-catechol was prepared from the total polyphenolic extract by semipreparative high pressure liquid chromatography with use of RP18 column and assayed by ultraviolet visible spectroscopy
- Purity > 99 % (NMR at 500 MHz) - Diluted in Phosphate buffer saline (PBS).


Ethical approval:
not specified
Exposure assessment:
Details on exposure:
* Cell viability and growth assay:
Cells were cultured for a total of 5 days in presence of catechol. Cell growth and viability were measured by the tetrazolium salt assay.
All experiments were performed at least 3 times in triplicates.

* H2O2 treatment:
Cells were seeded in 24-well plates at an initial density of 1.5 x 10E5 cells/well. After 24 h, medium was replaced, FBS was omitted, and catechol was introduced (10E-8 M). 24 h later, medium containing different concentrations of H2O2 (0.05-5.0 mM) was provided. After 3 h at 37 °C cells were washed with PBS and their viability was determined.

* Reactive Oxygen species generation was determined by flow cytometry.

* Androgen binding assay: LNCaP cells (1.5 x 10E5) were seeded in 24-well plates. After 24 h, binding was performed on whole cells in serum-free PBS. 435 fmol of [3H]testosterone were introduced in 50 µl PBS, together with non labelled testosterone and catechol (10E-12 to 10E-6 M).
After overnight exposure, the bound radioactivity was counted in scintillation counter. Binding was repeated at least 3 times in duplicates.

* Determination of NO production and the determination of inducible NO synthase activity were also performed.

Results and discussion

* Cell proliferation:
Catechol was found to significantly inhibit the proliferation of PC3 cells. LNCaP cells were less sensitive to this effect. In DU145 cells, catechol produced only partial inhibition.
* Catechol acts preferentially on the two cell lines possessing non-functional (PC3) or functional (LNCaP) androgen receptor. Results of competition experiments indicate that catechol is a very weak competitor of androgen binding.
* Preincubation with catechol resulted in an increased resistance of DU145 and PC3 cells to H2O2 toxicity. In LNCaP cells, this effect was observed only at H2O2 concentrations < 1 mM.
* Production of ROS: In PC3 cells, a significant decrease of ROS production was observed. At long incubation time (> 40 min), this effect was also observed in the DU145 cell line.
LNCaP cells did not show any modification of ROS production after catechol preincubation.
* Production of NO: Catechol decreases the production/secretion of NO.

Applicant's summary and conclusion