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Ecotoxicological information

Biological effects monitoring

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Administrative data

Endpoint:
biological effects monitoring
Type of information:
experimental study
Adequacy of study:
other information
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
other: No particular guideline is needed, and the test is relatively well described.

Data source

Reference
Reference Type:
publication
Title:
Cytotoxicity of phenolic compounds on Dicentrarchus labrax erythrocytes.
Author:
Bogé G., Roche H.
Year:
1996
Bibliographic source:
Bull. Environ. Contam. Toxicol., 57, 171-178.

Materials and methods

Principles of method if other than guideline:
In vitro effects.
GLP compliance:
not specified
Type of study / information:
In vitro effects using fish erythrocytes

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyrocatechol
EC Number:
204-427-5
EC Name:
Pyrocatechol
Cas Number:
120-80-9
Molecular formula:
C6H6O2
IUPAC Name:
pyrocatechol
Details on test material:
Purchased from Riedel-de-Haën (Seelze, Germany).

Results and discussion

Any other information on results incl. tables

- Hemolysis appeared from 10 µM catechol after 24-hr incubation. At 100 µM, 10% of the red blood cells were hemolysed. At 1 mM, the hemolysis rate was 70%.
The decrease of ATP content was 90% at 100 µM and ca. 100% at 1 mM.
- At 100 µM, pyrocatechol significantly increased the activities of MnSOD, peroxidase and glutathione peroxidase.
This stimulation persisted at 10 µM for MnSOD activity.
Catalase activity decreased.

According to the authors, catechol interacts with the production of oxygen radicals. This hypothesis is supported by the fact that during autoxidation of catechol, such free radicals are formed.

Applicant's summary and conclusion