Pyrocatechol

Long-term toxicity to aquatic invertebrates

Administrative data

Endpoint:

long-term toxicity to aquatic invertebrates

Type of information:

experimental study

Adequacy of study:

disregarded due to major methodological deficiencies

Reliability:

3 (not reliable)

Rationale for reliability incl. deficiencies:

significant methodological deficiencies

Remarks:

The experimental conditions and the obtained results were partially described. The tested organism is not a standardised ones. Furthermore, no standardised method seemed to be followed. As a result, this study cannot be judged as fully reliable to assess the long-term toxicity on aquatic invertebrates.

Data source

Reference

Reference Type:

publication

Title:

Toxicological effects produced by some 1,3-benzodioxoles, catechols, and quinones in Culex mosquito larvae.

Author:

Desmarchelier J.M., Fukuto TR.

Year:

1974

Bibliographic source:

J. Econ. Entomol., 67(2), 153-158.

Materials and methods

Principles of method if other than guideline:

The chronic toxicity of catechol was assessed on mosquito larvae during a 21-day exposure period.

GLP compliance:

not specified

Test material

Test material information

Constituent 1

Reference substance name:

Pyrocatechol

EC Number:

204-427-5

EC Name:

Pyrocatechol

Cas Number:

120-80-9

Molecular formula:

C6H6O2

IUPAC Name:

pyrocatechol

Details on test material:

Catechol was synthesised as described by Desmarchelier et al. (1973). It was purified by recrystallization or sublimation prior to use. Structure was confirmed by NMR and usually by mass spectrometry.

Sampling and analysis

Analytical monitoring:

no

Test solutions

Vehicle:

yes

Details on test solutions:

- Freshly prepared solutions (0.25 mL) of the compound dissolved in distilled acetone were added to 100 mL water in a 250 mL glass beaker containing 20 late 3rd-instars.
Control experiments were made by the addition of acetone alone (0.25 or 1 mL).

Test organisms

Test organisms (species):

other: Culex pipiens quinquefasciatus (larvae)

Details on test organisms:

TEST ORGANISM
- Common name: Southern house mosquito
- Life-stage : Larvae (late 3rd-instars)
- Feeding during test
- Food type: Larvae were fed on a finely ground mixture of 3 parts Purina laboratory chow and 1 part brewer's yeast
- Amount: No data
- Frequency: Daily feeding
No further data

ACCLIMATION : No data

QUARANTINE (wild caught) : No data

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: No data

Study design

Test type:

not specified

Water media type:

freshwater

Total exposure duration:

21 d

Test conditions

Hardness:

No data

Test temperature:

24°C

pH:

No data

Dissolved oxygen:

No data

Salinity:

Not applicable

Nominal and measured concentrations:

No data

Details on test conditions:

The bioassay method of mosquito larvae, Culex pipiens quinquefasciatus Say, was essentially that of Sacher (1971) for continuous exposure except that a larger volume of water was used.
- The water was not changed during the course of the experiments but the level was adjusted daily to the initial volume.
- The number of live and dead larvae, pupae, and adults was estimated daily. Pupal and larval moults also were counted daily, and the moults were removed.
- % 24-h larval mortality, % total larval mortality, % dead pupae, % dead adults, and % live adults were recorded.
Larvae that had not progressed to the pupal stage after 21 days were classified as dead.

Results and discussion

Effect concentrations

Duration:

21 d

Dose descriptor:

other: LC80

Effect conc.:

1 mg/L

Basis for effect:

mortality

Details on results:

In catechol experiments, larvae that failed to respond to stimuli of light, sound, and motion after 24 h were often normal after 48 h. 7 of 20 larvae treated with catechol at 1 ppm were monitored and considered "dead" at 24 h, but only 2 were dead at 48 and 72 h post treatment. However, 80% of the larvae were dead before the end of the bioassay period (21 days).