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Ecotoxicological information

Long-term toxicity to aquatic invertebrates

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Administrative data

Endpoint:
long-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
disregarded due to major methodological deficiencies
Reliability:
3 (not reliable)
Rationale for reliability incl. deficiencies:
other: see 'Remark'
Remarks:
The experimental conditions and the obtained results were partially described. The tested organism is not a standardised ones. Furthermore, no standardised method seemed to be followed. As a result, this study cannot be judged as fully reliable to assess the long-term toxicity on aquatic invertebrates.

Data source

Reference
Reference Type:
publication
Title:
Toxicological effects produced by some 1,3-benzodioxoles, catechols, and quinones in Culex mosquito larvae.
Author:
Desmarchelier J.M., Fukuto TR.
Year:
1974
Bibliographic source:
J. Econ. Entomol., 67(2), 153-158.

Materials and methods

Principles of method if other than guideline:
The chronic toxicity of catechol was assessed on mosquito larvae during a 21-day exposure period.
GLP compliance:
not specified

Test material

Constituent 1
Chemical structure
Reference substance name:
Pyrocatechol
EC Number:
204-427-5
EC Name:
Pyrocatechol
Cas Number:
120-80-9
Molecular formula:
C6H6O2
IUPAC Name:
pyrocatechol
Details on test material:
Catechol was synthesised as described by Desmarchelier et al. (1973). It was purified by recrystallization or sublimation prior to use. Structure was confirmed by NMR and usually by mass spectrometry.

Sampling and analysis

Analytical monitoring:
no

Test solutions

Vehicle:
yes
Details on test solutions:
- Freshly prepared solutions (0.25 mL) of the compound dissolved in distilled acetone were added to 100 mL water in a 250 mL glass beaker containing 20 late 3rd-instars.
Control experiments were made by the addition of acetone alone (0.25 or 1 mL).

Test organisms

Test organisms (species):
other: Culex pipiens quinquefasciatus (larvae)
Details on test organisms:
TEST ORGANISM
- Common name: Southern house mosquito
- Life-stage : Larvae (late 3rd-instars)
- Feeding during test
- Food type: Larvae were fed on a finely ground mixture of 3 parts Purina laboratory chow and 1 part brewer's yeast
- Amount: No data
- Frequency: Daily feeding
No further data

ACCLIMATION : No data

QUARANTINE (wild caught) : No data

METHOD FOR PREPARATION AND COLLECTION OF EARLY INSTARS OR OTHER LIFE STAGES: No data

Study design

Test type:
not specified
Water media type:
freshwater
Total exposure duration:
21 d

Test conditions

Hardness:
No data
Test temperature:
24°C
pH:
No data
Dissolved oxygen:
No data
Salinity:
Not applicable
Nominal and measured concentrations:
No data
Details on test conditions:
The bioassay method of mosquito larvae, Culex pipiens quinquefasciatus Say, was essentially that of Sacher (1971) for continuous exposure except that a larger volume of water was used.
- The water was not changed during the course of the experiments but the level was adjusted daily to the initial volume.
- The number of live and dead larvae, pupae, and adults was estimated daily. Pupal and larval moults also were counted daily, and the moults were removed.
- % 24-h larval mortality, % total larval mortality, % dead pupae, % dead adults, and % live adults were recorded.
Larvae that had not progressed to the pupal stage after 21 days were classified as dead.

Results and discussion

Effect concentrations
Duration:
21 d
Dose descriptor:
other: LC80
Effect conc.:
1 mg/L
Basis for effect:
mortality
Details on results:
In catechol experiments, larvae that failed to respond to stimuli of light, sound, and motion after 24 h were often normal after 48 h. 7 of 20 larvae treated with catechol at 1 ppm were monitored and considered "dead" at 24 h, but only 2 were dead at 48 and 72 h post treatment. However, 80% of the larvae were dead before the end of the bioassay period (21 days).

Applicant's summary and conclusion