Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 203-537-0 | CAS number: 107-96-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro cytogenicity / chromosome aberration study in mammalian cells
- Remarks:
- Type of genotoxicity: chromosome aberration
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 26 April 2007 to 17 July 2007
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: Guideline study; GLP, no deviations
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 007
- Report date:
- 2007
Materials and methods
Test guidelineopen allclose all
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 473 (In Vitro Mammalian Chromosome Aberration Test)
- Version / remarks:
- adopted 1997
- Deviations:
- no
- Qualifier:
- according to guideline
- Guideline:
- EU Method B.10 (Mutagenicity - In Vitro Mammalian Chromosome Aberration Test)
- Version / remarks:
- adopted 2000
- Deviations:
- no
- GLP compliance:
- yes (incl. QA statement)
- Type of assay:
- in vitro mammalian chromosome aberration test
Test material
- Reference substance name:
- 3-mercaptopropionic acid
- EC Number:
- 203-537-0
- EC Name:
- 3-mercaptopropionic acid
- Cas Number:
- 107-96-0
- Molecular formula:
- C3H6O2S
- IUPAC Name:
- 3-sulfanylpropanoic acid
- Details on test material:
- - Name of test material (as cited in study report): 3-Mercaptopropionic acid
- Physical state: Clear colourless liquid
- Analytical purity: 99.5% (GC)
- Purity test date: 2007-04-18
- Lot/batch No.: 19185
- Expiration date of the lot/batch: 2008-04-17
- Stability under test conditions: no data
- Storage condition of test material: ~4°C in the dark
Constituent 1
Method
Species / strain
- Species / strain / cell type:
- lymphocytes: human
- Details on mammalian cell type (if applicable):
- - Type and identity of media: MEM
- Properly maintained: not applicable, primary culture
- Periodically checked for Mycoplasma contamination: not applicable, primary culture
- Periodically checked for karyotype stability: not applicable, primary culture
- Periodically "cleansed" against high spontaneous background: not applicable, primary culture
- Metabolic activation:
- with and without
- Metabolic activation system:
- S9 was prepared from the livers of male Sprague-Dawley rats weighing ~250 g. These had received three daily oral doses of a mixture of phenobarbitone (80 mg/kg) and ß-naphthoflavone (100 mg/kg), prior to S9 preparation on the fourth day.
- Test concentrations with justification for top dose:
- 4-h exposure: 33.1 to 1060 µg/mL
24-h exposure: 33.1 to 530 µg/mL - Vehicle / solvent:
- - Vehicle(s)/solvent(s) used: culture medium
Controlsopen allclose all
- Untreated negative controls:
- no
- Negative solvent / vehicle controls:
- yes
- True negative controls:
- no
- Positive controls:
- yes
- Positive control substance:
- mitomycin C
- Remarks:
- without S9
Migrated to IUCLID6: 0.4 and 0.2 µg/mL for 4- and 24-h exposure
- Positive control substance:
- cyclophosphamide
- Remarks:
- with S9
Migrated to IUCLID6: 5 µg/mL
- Details on test system and experimental conditions:
- METHOD OF APPLICATION: in medium
DURATION
- Exposure duration: 4, 24 h
- Fixation time (start of exposure up to fixation or harvest of cells): 24 h
SPINDLE INHIBITOR (cytogenetic assays): Colcemid 0.1 µg/mL
STAIN (for cytogenetic assays): 5% Gurrs Giemsa
NUMBER OF REPLICATIONS: 2
NUMBER OF CELLS EVALUATED: 200 per concentration
DETERMINATION OF CYTOTOXICITY
- Method: mitotic index
OTHER EXAMINATIONS:
- Determination of polyploidy: yes
- Determination of endoreplication: yes - Evaluation criteria:
- A positive response was recorded for a particular treatment if the % cells with aberrations, excluding gaps, markedly exceeded that seen in the concurrent control, either with or without a clear concentration-relationship. For modest increases in aberration frequency a concentration response relationship is generally required and appropriate statistical tests may be applied in order to record a positive response.
- Statistics:
- Fisher's Exact test.
Results and discussion
Test results
- Species / strain:
- lymphocytes: human
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- no cytotoxicity nor precipitates, but tested up to recommended limit concentrations
- Remarks:
- 10 mM
- Vehicle controls validity:
- valid
- Untreated negative controls validity:
- not applicable
- Positive controls validity:
- valid
- Additional information on results:
- RANGE-FINDING/SCREENING STUDIES:
The concentration range for the Preliminary Toxicity Test was 16.5 to 1060 µg/mL. The maximum concentration was the 10 mM concentration. No precipitate of the test material was observed at the end of the exposure period in any of the three exposure groups. Microscopic assessment of the slides prepared from the exposed cultures showed that metaphase cells were present up to 1060 µg/mL in the 4(20)-hour exposures both in the presence and absence of metabolic activation (S9). The maximum concentration with metaphases present in the 24-hour continuous exposure was 1060 µg/mL, though it should be noted that frequency of metaphases was very low. The test material induced some evidence of toxicity.
COMPARISON WITH HISTORICAL CONTROL DATA: within historical reference range - Remarks on result:
- other: all strains/cell types tested
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
All vehicle (solvent) controls had frequencies of cells with
aberrations within the range expected for normal human
lymphocytes. All the positive control materials induced
statistically significant increases in the frequency of
cells with aberrations indicating the satisfactory
performance of the test and of the activity of the
metabolising system. The test material was moderately toxic
and did not induce any statistically significant increases
in the frequency of cells with aberrations, in either of two
separate experiments, using a concentration range that
included a concentration level that induced approximately
50% mitotic inhibition.
MPA produced some evidence of toxicity at 1060 µg/mL in the preliminary toxicity test, as well as in the chromosome aberration test.
Table 1: Experiment 1 - 4 h treatment, 20 h fixation - Without Metabolic Activation
Concentration |
Mitotic index [%] |
Polyploid cells [%] |
Aberrant cells (%) |
|
incl. gaps |
excl. gaps |
|||
0 |
100 |
0.0 |
0.5 |
0.0 |
265 |
91 |
0.0 |
1.5 |
0.5 |
530 |
75 |
0.5 |
0.0 |
0.0 |
1060 |
71 |
0.0 |
2.0 |
1.5 |
MMC, 0.4 |
60 |
0.0 |
35.3 |
33.3*** |
MMC: Mitomycin C
*** p<0.001
Table 2: Experiment 1- 4 h treatment, 20 h fixation - With Metabolic Activation
Concentration |
Mitotic index [%] |
Polyploid cells [%] |
Aberrant cells (%) |
|
incl. gaps |
excl. gaps |
|||
0 |
100 |
0.0 |
1.0 |
0.5 |
265 |
100 |
0.0 |
0.5 |
0.5 |
530 |
69 |
0.0 |
0.5 |
0.5 |
1060 |
70 |
0.0 |
1.5 |
1.5 |
CP, 5 |
30 |
0.0 |
38.8 |
33.0*** |
CP: Cyclophosphamide
*** p<0.001
Table 3: Experiment 2- 20 h treatment, 20 h fixation - Without Metabolic Activation
Concentration |
Mitotic index [%] |
Polyploid cells [%] |
Aberrant cells (%) |
|
incl. gaps |
excl. gaps |
|||
0 |
100 |
0.5 |
0.5 |
0.5 |
66.25 |
104 |
0.0 |
2.0 |
0.5 |
132.5 |
82 |
0.0 |
2.5 |
0.5 |
265 |
36 |
0.0 |
3.0 |
2.0 |
MMC, 0.4 |
50 |
0.0 |
17.0 |
16.0*** |
MMC: Mitomycin C
*** p<0.001
Table 4: Experiment 2- 4 h treatment, 20 h fixation - With Metabolic Activation
Concentration |
Mitotic index [%] |
Polyploid cells [%] |
Aberrant cells (%) |
|
incl. gaps |
excl. gaps |
|||
0 |
100 |
0.0 |
0.0 |
0.0 |
265 |
178 |
0.0 |
2.0 |
1.5 |
530 |
142 |
0.0 |
0.5 |
0.5 |
1060 |
161 |
0.0 |
2.5 |
0.5 |
CP, 5 |
54 |
0.0 |
28.0 |
24.0*** |
CP: Cyclophosphamide
*** p<0.001
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results (migrated information):
negative with and without metabolic activation
The test material is non-clastogenic to human lymphocytes in vitro, with and without metabolic activation.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.

EU Privacy Disclaimer
This website uses cookies to ensure you get the best experience on our websites.