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EC number: 203-537-0 | CAS number: 107-96-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Short-term toxicity to aquatic invertebrates
Administrative data
Link to relevant study record(s)
- Endpoint:
- short-term toxicity to aquatic invertebrates
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Study period:
- 1999-10-20 to 2000-03-03
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- guideline study
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
- GLP compliance:
- yes (incl. QA statement)
- Remarks:
- Hessisches Ministerium fuer Umwelt, Energie, Jugend, Familie und Gesundheit
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were drawn from the test solutions and analyzed on the same day of collection.
- Vehicle:
- no
- Details on test solutions:
- dilution water according to DIN 38412, part 11
The test substance was introduced into the dilution water whilst stirring by aid of a magnetic stirrer for 10 min. The maximum concentration applied was 1000 mg/L. Within the definitive main test concentrations of 100, 50, 25, 12.5, and 6.25 mg/L of the test substance were used. - Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Daphnia magna STRAUS
- Source: bred in the laboratory of the test facility (originally derived from Dr. Knie, LWA Duesseldorf)
- Age at study initiation: not older than 24 hours
- Method of breeding:
- Feeding during test: test animals were not fed during the test - Test type:
- static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 48 h
- Test temperature:
- 20 +/- 1°C;
determined at the beginning and the end of the test in each treatment group, see below ("remarks on results including tables and figures") - pH:
- determined at the beginning and the end of the test in each treatment group, see below ("remarks on results including tables and figures")
- Dissolved oxygen:
- determined at the beginning and the end of the test in each treatment group, see below ("remarks on results including tables and figures")
- Nominal and measured concentrations:
- nominal test item concentrations (mg/L): 100, 50, 25, 12.5, 6.25, and 0 (control);
the single measured test item concentrations are not reported (the biological effects based on measured concentrations are reported; see below) - Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Type (delete if not applicable): closed (glass stoppers)
- Material, size, headspace, fill volume: glass, 250 mL, no headspace, approx. 290 mL
- Aeration: no active aeration
- No. of organisms per vessel: 5
- No. of vessels per concentration (replicates): 4
- No. of vessels per control (replicates): 4
- No. of vessels per vehicle control (replicates): no vehicle used
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: dilution water according to DIN 38412, part 11
OTHER TEST CONDITIONS
- Photoperiod: light : dark : 16:8 hours
EFFECT PARAMETERS MEASURED: immobility
TEST CONCENTRATIONS
- Spacing factor for test concentrations: 2.0 - Reference substance (positive control):
- yes
- Remarks:
- potassium dichromate (tested in a separate study)
- Key result
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 17 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Duration:
- 48 h
- Dose descriptor:
- EC50
- Effect conc.:
- 9 mg/L
- Nominal / measured:
- meas. (geom. mean)
- Conc. based on:
- test mat.
- Basis for effect:
- mobility
- Remarks on result:
- other: geom. mean of meas. conc. between t=0h and t=48h
- Details on results:
- corresponding EC values based on nominal test item concentrations:
48 hour EC0 / EC50 / EC100 (mg/L): 6.5 / 17 / 46
24 hour EC0 / EC50 / EC100 (mg/L): 8.3 / 31 / 110 - Results with reference substance (positive control):
- A reference substance was not tested as a direct parallel to the study with the test item.
Nevertheless, the sensitivity of the test system towards toxic substances is verified once a month with potassium dichromate. The value of the EC50 in this period of time was 1.5 mg/L K-dichromate. - Reported statistics and error estimates:
- Numbers of immobilized Daphnia were plotted against the respective concentration. Using a validated computer program on the basis of EXCEL 5.0, the EC50 value was determined as well as the EC100 and the NOEC together with the 95% confidence limits.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The following EC-values were calculated on basis of the nominal concentrations being tested: 24 h EC50 = 31 mg/L, 48 h EC50 = 17 mg/L
- Executive summary:
3-Mercaptopropionic acid was tested for acute toxicity towards Daphnia according to OECD 202, Part I. In order to investigate the influence of the test substance towards the daphnia, the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnia was observed at concentrations >12.5 mg/L. The following EC-values were calculated on basis of the nominal concentrations being tested: 24 h EC50 = 31 mg/L, 48 h EC50 = 17 mg/L
Within TOC-analyses performed with a test solution freshly prepared in tap water, and after 96h (this is the time schedule for a fish toxicity test), it could be shown, that the organic matter of the test substance remained constant in the aqueous phase. Upon chemical analyses of test solutions performed with freshly prepared test solutions and after 48h of incubation it could be demonstrated, that the test substance was partially oxidized, and thus there are two components, the original test substance and the oxidation product, present in the test solution. It cannot be distinguished between a toxic effect of the original test substance and the oxidation product because they are both present in the test solution representing also a dynamic process. For this reason, the results of this test are based on the nominal concentrations tested. As an alternative calculation (not recommended by the OECD Guideline 202 Part D using the measured concentration of the original test substance 3-mercaptopropionic acid, the following EC-values were calculated: 24 hEC50 = 14 mg/L, 48 h EC50 = 4 mg/LThese values do not represent the real environmental conditions because the test substance is rapidly oxidized by the oxygen content in the aqueous phase.
Reference
Immobilization data in the time course of the test (20 Daphnia per test concentration):
Conc. of test substance (mg/L) |
Ratio immobilized Daphnia (%) |
|
Control (0) |
0 [0/20] |
0 [0/20] |
100 |
100 [20/20] |
100 [20/20] |
50 |
75 [ 15/20] |
100 [20/20] |
25 |
40 [8/20] |
80 [16/20] |
12.5 |
0 [0/20] |
25 [5/20] |
6.25 |
0 [0/20] |
0 [0/20] |
Numbers in brackets are the absolute numbers of animals
immobilized at each concentration.
Physical parameters data in the time course of the test:
Conc. of test substance (mg/L) |
PH value |
O2 content (mg O2/L) |
Temperature (°C) |
|||
0 h |
48 h |
0 h |
48 h |
0 h |
48 h |
|
Control (0) |
7.25 |
7.16 |
8.0 |
7.8 |
19.0 |
21.3 |
100 |
4.95 |
5.48 |
8.1 |
7.8 |
19.0 |
21.3 |
50 |
6.77 |
6.98 |
8.1 |
7.8 |
19.0 |
21.3 |
25 |
6.97 |
7.20 |
8.2 |
7.8 |
19.0 |
21.3 |
12.5 |
7.05 |
7.24 |
8.2 |
7.8 |
19.0 |
21.3 |
6.25 |
7.09 |
7.30 |
8.2 |
7.8 |
19.0 |
21.3 |
Calculated EC values based on the geometric mean measured concentrations (as recommended by OECD Guidance Document No. 23):
|
24 h |
48 h |
EC0 |
5.2 mg/L |
2.4 mg/L |
EC50 |
21 mg/L |
9 mg/L |
EC100 |
88 mg/L |
35 mg/L |
(For each concentration, the geometric mean for the measured
concentrations between t0 and t48 h were used)
Calculated EC values based on nominal concentrations:
|
24 h |
48 h |
EC0 |
8.3 mg/L |
6.5 mg/L |
EC50 |
31 mg/L |
17 mg/L |
EC100 |
110 mg/L |
46 mg/L |
Chemical analysis of the test solutions performed with freshly prepared test solutions and after 48 h of incubation demonstrated that the test substance was partially oxidized, and thus there are two components, the original test substance and the oxidation product, present in the test solution.
Sampling time (h) |
Concentration of Test Substance (mg/L) |
|
Nominal |
Measured |
|
0 |
0 (control) |
nd |
0 |
100 |
104 |
0 |
50 |
53 |
0 |
25 |
24 |
48 |
0 (control) |
nd |
48 |
100 |
90 |
48 |
50 |
32 |
48 |
25 |
6 |
nd = no peaks detected
The samples were drawn from the test solutions and analyzed
the same day of collection.
All validity criteria for the test were met.
Description of key information
EC50 (48 h) = 17 mg/L (Daphnia magna)
Key value for chemical safety assessment
Fresh water invertebrates
Fresh water invertebrates
- Dose descriptor:
- EC50
- Effect concentration:
- 17 mg/L
Additional information
3-Mercaptopropionic acid was tested for acute toxicity towards Daphnia according to OECD 202, Part I. In order to investigate the influence of the test substance towards the daphnia, the swimming behaviour of the animals was recorded. Under the conditions used for the test, immobilisation of the daphnia was observed at concentrations >12.5 mg/L. The following EC-values were calculated on basis of the nominal concentrations being tested: 24 h EC50 = 31 mg/L, 48 h EC50 = 17 mg/L
Within TOC-analyses performed with a test solution freshly prepared in tap water, and after 96h (this is the time schedule for a fish toxicity test), it could be shown, that the organic matter of the test substance remained constant in the aqueous phase. Upon chemical analyses of test solutions performed with freshly prepared test solutions and after 48h of incubation it could be demonstrated, that the test substance was partially oxidized, and thus there are two components, the original test substance and the oxidation product, present in the test solution. It cannot be distinguished between a toxic effect of the original test substance and the oxidation product because they are both present in the test solution representing also a dynamic process. For this reason, the results of this test are based on the nominal concentrations tested. As an alternative calculation (not recommended by the OECD Guideline 202 Part D using the measured concentration of the original test substance 3-mercaptopropionic acid, the following EC-values were calculated: 24 hEC50 = 14 mg/L, 48 h EC50 = 4 mg/L
These values do not represent the real environmental conditions because the test substance is rapidly oxidized by the oxygen content in the aqueous phase.
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