3-mercaptopropionic acid

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1973

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Similar to guideline study; not GLP. Only 3 females per dose level. Classification is possible based on study results.

Cross-reference

Reason / purpose for cross-reference:

reference to same study

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1973

Report date:

1973

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

Only females were tested. Only 3 animals per dose were tested.

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

3-mercaptopropionic acid

EC Number:

203-537-0

EC Name:

3-mercaptopropionic acid

Cas Number:

107-96-0

Molecular formula:

C3H6O2S

IUPAC Name:

3-sulfanylpropanoic acid

Test animals

Species:

rat

Strain:

not specified

Sex:

female

Details on test animals or test system and environmental conditions:

no data

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

water

Details on oral exposure:

VEHICLE
- Concentration in vehicle: 10%
- Amount of vehicle (if gavage): not reported
- Justification for choice of vehicle: water

MAXIMUM DOSE VOLUME APPLIED: not reported

Doses:

15.8, 31.6, 63, 126 or 252 mg/kg

No. of animals per sex per dose:

2-3

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days
- Frequency of observations and weighing: periodic observations, pre- and post-dose weighing
- Necropsy of survivors performed: yes, 24 h post dose in one animal per group
- Other examinations performed: clinical signs, body weight

Results and discussion

Effect levels

Effect levels 1

Sex:

female

Dose descriptor:

LD50

Effect level:

120 mg/kg bw

Based on:

test mat.

Remarks on result:

other: probit model

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

>= 63 - <= 126 mg/kg bw

Based on:

test mat.

Mortality:

see Table 1

Clinical signs:

other: No other signs of toxicity other than death were noted.

Gross pathology:

No visible lesions were found.

Any other information on results incl. tables

All animals survived following oral gavage doses of 15.8, 31.6 or 63 mg/kg.  Two of three died within 2 hours after
ingesting 126 mg/kg and all died within 1 hour after ingesting 252 mg/kg.
Using the probit model, the LD50 is approximately 120 mg/kg bw.
No signs of toxicity other than death were seen in these animals.  One animal from each of the three lowest dose
levels was necropsied 24 hours after exposure.  No visible lesions were found.  The surviving animals surpassed their
pre-exposure body weight by the end of the two week observation period.

 

Table 1: Acute oral toxicity of 3-MPA

Dose [mg/kg bw]	# dead / # treated	Time of Death
15.8	0/2	–
31.6	0/2	–
63	0/2	–
126	2/3	1 h, 2 h
252	3/3	less than 1 h

Applicant's summary and conclusion

Interpretation of results:

Category 3 based on GHS criteria

Conclusions:

EU: T, R25
GHS: Acute Oral Category 3

Executive summary:

In an acute oral toxicity study similar to the later established OECD TG 401 2-3  female rats/dose were administered a single dose of 3-MPA at 10% in water at dose levels of 15.8, 31.6, 63, 126 or 252 mg/kg. Animals were observed for 14 days.

All animals survived following oral gavage doses of 15.8, 31.6 or 63 mg/kg. Two of three died within 2 hours after ingesting 126 mg/kg and all died within 1 hour after ingesting 252 mg/kg.
Using the probit model, the LD50 is approximately 120 mg/kg bw.
No signs of toxicity other than death were seen in these  animals. One animal from each of the three lowest dose levels was necropsied 24 hours after exposure. No visible lesions were found. The surviving animals surpassed their pre-exposure body weight by the end of the two week observation period.