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EC number: 205-087-0
CAS number: 133-06-2
The results of the reproduction assessments during the definitive test are summarised in Table 2. In the control, the mean number of progeny produced per replicate was 550 compared with 228, 354, 412, 493, and 520 in the 1000, 562.4, 316.3, 177.9, and 100.1 mg a.i./kg soil treatment concentrations of Merpan 80 WDG, respectively. Statistically, the results for the 1000, 562.4 and 316.3 mg a.i./kg soil treatment concentrations differed significantly from the control (ANOVA, α = 0.05). Based on these results, the NOEC was 177.9 mg a.i./kg soil and the LOEC was 316.3 mg a.i./kg soil.
Table 2. The number of progeny produced by 28 DAT in the definitive test. The percentage reduction in the reproductive performances of each treated group compared to the control group is also given
(mg a.i./kg soil)
Mean no. progeny
per replicate a)
% change relative to
the control b)
Merpan 80 WDG
The test item in this study was Merpan 80 WDG, a water-dispersible granule formulation containing the active ingredient captan (nominally 80% w/w). The aim of this study was to determine under laboratory test conditions whether this test item had harmful effects on the springtail Folsomia candida (Willem) (Collembola, Isotomidae). This is considered to be a suitable test species for determining the effects of pesticides on soil-active Collembola in the agricultural ecosystem (Wiles et al., 1998). The test design was in line with current European guidance documents (Barrett et al., 1994; Candolfi et al., 2001), that themselves meet the requirements of EU Commission Directive 96/12/EC (1996). The test method was based on OECD laboratory test guideline 232 (OECD, 2009), which supersedes ISO guideline 11267 (1999). This involved the confinement ofspringtails in an artificial soil substrate containing 5% peat, following its treatment with the test item. The survival and reproduction of the springtails was then assessedover a period of 28 days.
An initial range-finding test was carried out using concentrations equivalent to 1000, 100, 10, 1 and 0.1 mg a.i./kg soil dry weight (based on measured a.i. content).A definitive test was carried out, in which the test item was evaluated at five concentrations, equivalent to 1000, 562.4, 316.3, 177.9, and 100.1 mg a.i./kg dry weight soil (based on measured a.i. content).
Control = purified waterIn the Control, 1% mortality (0% corrected mortality) was recorded.
Toxic reference item = Betosip 114 (Sipcam)In the toxic reference treatment, 74% mortality (74% corrected mortality) was recorded.
In the control, the mean number of progeny produced per replicate was 550 compared with 228, 354, 412, 493, and 520 in the 1000, 562.4, 316.3, 177.9, and 100.1 mg a.i./kg soil treatment concentrations of Merpan 80 WDG, respectively. Statistically, the results for the 1000, 562.4 and 316.3 mg a.i./kg soil treatment concentrations differed significantly from the control (ANOVA, α = 0.05). Based on these results, the NOEC was 177.9 mg a.i./kg soil and the LOEC was 316.3 mg a.i./kg soil.
The 20 µg bee-1dose
level of the formulation required the application of two 1 µL drops.
Therefore this treatment was not strictly comparable with the others
which received 1 µL. Consequently, it was excluded from the statistical
analysis. This had no effect on the conclusions reached as to the LD50
value or the NOEL because of the total lack of observed effects on bees
in this treatment.
There was no mortality
attributable to technical captan so that the 24- hour LD50s were greater
than the highest dose tested: 200 and 20 µg bee-1,
respectively. The 48- hour results were similar.
There were no treatment
related sublethal effects observed in bees treated with technical
captan. Using the combined mortality and sub-lethal effects values, the
NOELs were therefore equal to or greater than the highest dose tested
(200 and 20 µg bee-1, respectively).
There was no mortality or
sublethal effects attributable to either technical Captan. Therefore,
the 24- and 48- hour LD50 values were greater than and the NOELs equal
to or greater than the highest doses tested: 100 µg bee-1for
Overall there were no
treatment-related mortalities or sublethal effects of captan in the oral
tests at any dose at any
assessment time. Mortality following treatment with dimethoate occurred
at expected levels.
Laboratory tests was carried
out on captan technical. There were two tests for each route of
administration for technical captan: a rangefinder test and a definitive
test based on the results of the rangefinder test.
The 24 hour contact LD50
values for technical captan was >200 and >20 µg bee-1,
respectively. The 24-hour oral LD50 values for technical captan was >100
and >20 µg bee-1. The No Observed Effect Levels
(NOELs) were greater than, or equal to, the highest dose tested in each
obtained for dimethoate tested concurrently as a toxic standard
indicated that the bees were reacting normally under the test conditions
1) Toxicity to terrestrial arthropods (Apis mellifera): LD50 (oral, 24h and 48h) >100 µg captan/bee,
LD50 (contact, 24h and 48h) >200 µg captan/bee for worker Honey bees (Apis mellifera),
Guideline UK MAFF PSPS Working Document D3 (1979).
2) Toxicity to terrestrial arthropods (springtail Folsomia candida):
LC50 (median lethal concentration): > 1000 mg a.i./kg soil dry weight.
With respect to progeny, the 28-day EC50 (median effect concentration): 807.9 mg a.i./kg soil (95% confidence limits = 648.3 and 1103.6 mg a.i./kg soil).
Based on statistical comparisons, the NOEC for effects on reproduction: 177.9 mg a.i./kg soil dry weight.
LOEC: 316.3 mg a.i./kg soil dry weight.
The result show that the 24-hour contact LD50 values for technical captan was >200 and >20 µg bee-1, respectively. These results indicate that the technical material is 'virtually non-toxic' to bees in the laboratory under the conditions of these tests (using the classification of the International Commission for Bee Botany-Second Symposium on harmonisation of methods for testing the toxicity of pesticides to bees, Hohenheim, West Germany, 21-23 September 1982). There were no treatment effects at the doses tested there is no evidence of any difference in the level of safety. There were no treatment related sublethal effects for the technical material so that the NOEL were ≥200 and ≥20 µg ai bee-1,respectively. There were no significant changes in the results in either the contact or the oral tests after 48 hours compared with 24 hours indicating that there were no delayed effects.
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