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Toxicity to terrestrial plants

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Reference
Endpoint:
toxicity to terrestrial plants: long-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
20 September 2011 to 11 Oktober 2011
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 227 (Terrestrial Plant Test: Vegetative Vigour Test)
Deviations:
no
GLP compliance:
yes
Analytical monitoring:
yes
Vehicle:
yes
Remarks:
tap water
Details on preparation and application of test substrate:
The test solution was prepared in tap water by directly weighing of the respective test item amounts
Species:
Avena sativa
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
oats, ivorySaaten Union GmbH, Eisenstrafie 12, D-30916 Isernhager HB
Species:
Allium cepa
Plant group:
Monocotyledonae (monocots)
Details on test organisms:
onion, ExhibitionHildSamen GmbH, Postfach 1161, D-71666 Marbach
Species:
Beta vulgaris
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
sugar beet, Beretta EPDKWS Saat AG, Grimsehlstr. 31. Postfach 1463, D-37555 Einbeck
Species:
Brassica napus
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
rape, DiggerKWS Saat AG, Grimsehlstr. 31. Postfach 1463, D-37555 Einbeck
Species:
Daucus carota
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
carrot, Merida f1Hild Samen GmbH, Postfach 1161, D-71666 Marbach
Species:
Glycine max (G. soja)
Plant group:
Dicotyledonae (dicots)
Details on test organisms:
soybean, Petrina PZOPZO Saat GmbH, Oberiimpurg 2, D-74523 SchwSbisch Hall
Test type:
vegetative vigour test
Study type:
laboratory study
Substrate type:
natural soil
Remarks:
with addition of 30% quatz sand
Limit test:
yes
Total exposure duration:
21 d
Test temperature:
14 - 22° C
Moisture:
43 - 96% relative humidty
Details on test conditions:
Bottom watering including fertilizer
Nominal and measured concentrations:
#1 nominal: 1619 mg/L; measured 1569 mg/L#2 nominal: 16191 mg/L; measured 15223 mg/L
Key result
Species:
Avena sativa
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Height
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Allium cepa
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Height
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Beta vulgaris
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Height
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Brassica napus
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Height
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Daucus carota
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Height
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Glycine max (G. soja)
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Height
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Avena sativa
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Fresh Weight
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Allium cepa
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Fresh Weight
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Beta vulgaris
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Fresh Weight
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Brassica napus
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Fresh Weight
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Daucus carota
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Fresh Weight
Remarks on result:
other: No phytotoxic effects
Key result
Species:
Glycine max (G. soja)
Duration:
21 d
Effect conc.:
1.92 kg/ha
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
phytotoxicity
Remarks:
Inhibition of Shoot Fresh Weight
Remarks on result:
other: No phytotoxic effects

Visual Phytotoxicity: The plants were observed on day 7, 14 and 21 for visual phytotoxic effects and number of dead plants. No phytotoxic effects were observed for all tested plant species at the limit application rate of 2.4 kg formulation/ha.

The percent Inhibition of shoot height and shoot fresh weight is given in Table 6.3.3-2  to Table 6.3.3-3.

 

Table 6.3.3-2 Inhibition of Shoot Height

Application

rate

[kg/ha]

Shoot height [cm]

Oats

Inhib.

[%]

Onion

Inhib.

[%]

Sugar

Beet

Inhib.

[%]

Rape

Inhib.

[%]

Carrot

Inhib.

[%]

Soybean

Inhib.

[%]

Control

60.9

-

37.8

-

16.5

-

23.2

-

29.8

-

43.1

-

2.4

60,7

0

39.8

-5

17.3

-5

22.4

3

30.7

-3

40.8

5

inhib. = Inhibition negative values = promoted grovrth

 

Table 6.3.3-3 Inhibition of Shoot Fresh Weight

Application

rate

[kg/ha]

Shoot Fresh Weight [g]

Oats

Inhib.

[%]

Onion

Inhib.

[%]

Sugar

Beet

Inhib.

[%]

Rape

Inhib.

[%]

Carrot

Inhib.

[%]

Soybean

Inhib.

[%]

Control

19.03

-

15.38

-

27.73

-

36.99

-

20.87

-

27.99

-

2.4

19.28

-1

14.64

5

32.04

-16

34.85

6

22.56

-8

27.93

-1

  inhib. = Inhibition negative values = promoted grovrth

Validity criteria fulfilled:
yes
Remarks:
OECD 227 (2006): The results of the control for all plant species met the required validity criteria
Conclusions:
No phytotoxic effects were observed for ail tested plant species at the limit application rate of 2.4 kg Captan/ha, corresponding to 1.92 kg a.i./ha (nominal). The test item did not cause inhibitory effects ≥ 10% on shoot height and shoot fresh weight (biomass) to all plant species at the application rate of 2.4 kg Captan/ha, corresponding to 1.92 kg a.i./ha (nominal).
Executive summary:

The phytotoxicity of captan to six terrestrial plant species was established in 21-day toxicity tests. Test plants comprised two monocotyledons (oats, Gramineae; onion, Liliaceae) and four dicotyledons (sugar beet, Chenopodiaceae; rape, Brassicaceae; carrot, umbelliferae; soybean, Leguminosae). The test was conducted with the limit application rate of 2.4 kg formulation/ha, corresponding to 1.92 kg captan/ha. The test item was applied at test start to plant foliage which had reached the 2-4 true leaf stage. Potential toxic effects of the test item were assessed on day 7, 14 and 21 by visual observations (phytotoxic effects and number of dead plants) and on day 21 additionally by shoot height and shoot fresh weight determination. No phytotoxic effects were observed for all tested plant at the limit application rate of 2.4 kg formulation/ha.

The test Item did not cause inhibitory effects ≥ 10 % on shoot height and shoot fresh weight (biomass) to all plant species at the application rate of 2.4 kg formulation/ha (Table 6.3.3-1).

 

Table 6.3.3-1 Inhibition of Shoot Height and Shoot Fresh Weight in Comparison to the Control

 

Inhibition [%]

 

Species

Shoot height

Shoot fresh weight

Oats

0

-1

Onion

-5

5

Sugar beet

-5

-16

Rape

3

6

Carrot

-3

-8

Soybean

5

-1

negative values = promoted growth

Description of key information

Toxicity to terrestrial plants: Vegetative Vigour Test (6 non-target plant species, 21 days post application), application rate: 2.4 kg formulation/ha ≡ 1.92 a.i./ha: no phytotoxic effects were observed, OECD (2007)

Key value for chemical safety assessment

Additional information

Captan is used as a fungicide on a wide range of crop groups and does not cause crop phytotoxicity. No phytotoxic effects were observed in a test with 6 different crop plants (including both monocotyledon and dicotyledon plant species) at dose rates of 1.92 kg captan/ha indicating a low risk to non-target organisms.