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EC number: 205-087-0
CAS number: 133-06-2
Analysis of stock solutions and test media: Measured concentrations of
captan in the stock solutions ranged from 91 to 102% of the nominal
values. The results of the toxicity test are based on nominal
concentrations of captan. The physical and chemical parameters of the
test media remained at expected values during the study (pH 7.8 to 8.5,
dissolved oxygen 83 to 106 % ASV, temperature 13.1 to 14.4°C and total
hardness 176 to 182 mg CaCO3/L).
Toxicity and behaviour: At 320 μg/L, all fish exhibited hyperventilation
within 15 minutes of exposure and were dead within four hours. At 145
μg/L, all fish were affected within two hours of exposure, exhibiting
hyperventilation, darkened pigmentation and/or lethargy after four
hours. After 24 hours, all fish were dead at 145 μg/L. At 66.1 μg/L,
most fish exhibited hyperventilation within two hours of exposure and
all were adversely affected after 24 and 48 hours Thereafter, some fish
appeared to recover, with five fish exhibiting no adverse symptoms at 96
hours. At 30.1 μg/L, no adverse effects were observed during the first
four hours of exposure but between 24 and 72 hours up to six fish
exhibited hyperventilation and five of these had recovered and appeared
normal after 96 hours. At 13.7 μg/L, all fish were normal for the first
four hours of exposure and between 24 and 72 hours, up to three fish
exhibited hyperventilation, but had fully recovered by 96 hours.
The results are summarised in Table 6.1.1 -1 and Table 6.1.1 -2.
6.1.1-1 Summary of
sub-lethal effects of captan on brown trout following 96-hour exposure
under static test conditions
Numbers affected (out of initial seven)
All fish appeared normal
6.1.1-2 Summary of mortality
of brown trout following 96-hour exposure to captan under static test
Cumulative mortality (number out of seven)
A study was performed to
assess the acute toxicity of captan Technical to brown trout (Salmo
trutta) under static (no renewal) exposure conditions. A static exposure
regime was selected in order to mimic a single application of the
product in the environment; under these conditions it is unlikely that
the exposure concentrations of captan would have been maintained during
the study since captan is not stable in water, particularly at low
concentrations and under alkaline conditions.
The study was conducted in
accordance with EEC method Part C, Method 1 "Acute Toxicity for Fish"
published in the regulation 440/2008 and the OECD Guideline for Testing
of Chemicals No. 203 "Fish, Acute Toxicity Test”.
Groups of seven juvenile
fish were exposed to captan Technical at nominal concentrations of 13.7,
30.1, 66.1, 145 and 320 µg/L (13.0, 28.7, 62.9, 138 and 305 µg a.i./l).
The test substance was dissolved in dimethylformamide before being added
to water (dechlorinated tap water, hardness ca. 180mg/L as CaCO2).
The concentrations of captan
in the solvent stock solutions, from which the test media were prepared,
were verified using an HPLC method of analysis and an analytical
standard of captan; the measured levels ranged between 91 and 102% of
their nominal values. Attempts to develop a suitable analytical method
to monitor the exposure concentrations in the aqueous media- during the
test were unsuccessful. Consequently, the test results have been
expressed in terms of their nominal values.
Observations of the fish
were made after approximately 0.25, 2, 4, 24, 48, 72 and 96 hours of
After 96 hours, the highest
nominal test concentration at which no mortality had occurred was 66.1
µg/l and the lowest at which there was 100% mortality was 145 µg/l.
Sub-lethal, treatment-related effects were exhibited by the fish at all
test levels, although five of the seven fish at 30.1 and 66.1 µg/L and
all of the fish at 13.7 µg/L had recovered by the end of the test and
Based on these findings, the
following values have been estimated:
96-hour LC50 value (95%
98 (66 &145) µg/l
96-hour LC10 value (95%
92 (57 &145) µg/l
93 (63 &138) µg a.i./l
88 (54&138) µg a.i./l
c.l.: confidence limits.
Short-term toxicity to fish: LC50 (96h) = 93 µg/L (adjusting for the purity of captan in captan technical (95.2%)), (with 95% confidence limits of 63 to 138 µg/L) for Salmo trutta (OECD 203 (Fish Acute Toxicity Test)
Acute toxicity on fish was determined with a study according to OECD guideline 203 in freshwater under static conditions. With a LC 50(96h) value of 93 µg/L on Salmo trutta fish was found to be the most sensitive species in aquatic system.
Based on the results captan is classified as acute toxic category 1 (H400) to fish.
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