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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 April to 25 April 1985
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
guideline study with acceptable restrictions
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Deviations:
no
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
yes
Details on preparation and application of test substrate:
Artificials soil: 69% industrial quartz sand, 20% kaolin clay, 10% sphagnum peat, 1% calcium carbonate, pH = 6.5
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
17 - 22° C
pH:
6.5
Reference substance (positive control):
yes
Remarks:
chloroacetamide
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 519.3 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
LC50(7d) = 35.8 mg/Kg; LC50(14d) = 20.8 mg/Kg

Chloroacetamide at a concentration of 3.0 to 243.0 mg/kg led to 27.5 - 100% mortality after 14 days. Mortality with captan is summarised in Table 6.3.1 -1.

Table 6.3.1-1 Summary of effects of captan on earthworms after 14 days exposure

Captan

concentration (mg/kg)1

% Mortality

7 days

14 days

0

0

0

5.2

0

7.5

15.6

5.0

10.0

52.0

5.0

20.00

155.9

10.0

37.5

519.3

7.5

32.5

1Nominal concentrations

Validity criteria fulfilled:
yes
Remarks:
Validity criteria of OECD Method 207 (1984) been fulfilled: The mortality in the controls does not exceed 10 per cent at the end of either test
Conclusions:
The 14-day LC50 of technical captan to earthworms was determined to be higher than 519.3 mg/kg.
Executive summary:

Test compound: Captan technical

 Protocol: OECD guidelines (1984) for testing of chemicals, entitled "Earthworm, Acute Toxicity Tests".

Test species: Earthworm, Eisenia foetida.

Duration of treatment: A Single soil treatment followed by a 14-day exposure period.

Treatments: Treatments included a negative control group and five groups treated with test compound at levels ranging from 5.2 mg/kg to 519.3 mg/kg .

Because of the limited number of mortalities in the test groups, it was not possible to determine LC50 values for Captan at 7 and 14 days. These values must lie in excess of the maximum dose level of 519.3 mg/kg.

The LC50 values for control group (chloroacetamide) were as follows:

 

Day 7:

LC50 value (ppm ai) 35.9 (95% confidence limits 20.6 – 68.9 ppm ai)

Day 14:

LC50 value (ppm ai) 20.8 (95% confidence limits 9.5 – 47.8 ppm ai)

 

The results indicate that Captan technical is of low toxicity to earthworms at concentrations of 5.2 to 519.3 mg/kg in soil.

Description of key information

Toxicity to soil macroorganisms except arthropods: LC50 (14 day) >519,3 mg/kg for earthworms (Eisenia foetida), OECD Guideline No. 207 (1984) "Earthworm acute toxicity test"

Key value for chemical safety assessment

Additional information

The results indicate that captan is of low toxicity to earthworms at concentrations of 5.2 to 519.3 mg/Kg in soil. LC50 was not reached.