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Ecotoxicological information

Toxicity to soil macroorganisms except arthropods

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Reference
Endpoint:
toxicity to soil macroorganisms except arthropods: short-term
Type of information:
experimental study
Adequacy of study:
key study
Study period:
11 April to 25 April 1985
Reliability:
1 (reliable without restriction)
Rationale for reliability incl. deficiencies:
guideline study
Qualifier:
according to guideline
Guideline:
OECD Guideline 207 (Earthworm, Acute Toxicity Tests)
Version / remarks:
1984
Deviations:
no
GLP compliance:
no
Specific details on test material used for the study:
- Physical state: white powder
- Purity: 90.5 %
- Batch number: 27878/611522
- Date of arrival: 30 January 1985
Analytical monitoring:
no
Vehicle:
yes
Details on preparation and application of test substrate:
Artificials soil: 69% industrial quartz sand, 20% kaolin clay, 10% sphagnum peat, 1% calcium carbonate, pH = 6.5
Test organisms (species):
Eisenia fetida
Animal group:
annelids
Study type:
laboratory study
Substrate type:
artificial soil
Limit test:
yes
Total exposure duration:
14 d
Test temperature:
17 - 22° C
pH:
6.5
Nominal and measured concentrations:
nominal concentrations in soil: 5.2, 15.6, 52.0, 155.9, 519.3 ppm/active ingredient
Reference substance (positive control):
yes
Remarks:
chloroacetamide
Key result
Duration:
14 d
Dose descriptor:
LC50
Effect conc.:
> 519.3 mg/kg soil dw
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Results with reference substance (positive control):
LC50(7d) = 35.8 mg/Kg; LC50(14d) = 20.8 mg/Kg

Chloroacetamide at a concentration of 3.0 to 243.0 mg/kg led to 27.5 - 100% mortality after 14 days. Mortality with captan is summarised in Table 6.3.1 -1.

Table 6.3.1-1 Summary of effects of captan on earthworms after 14 days exposure

Captan

concentration (mg/kg)1

% Mortality

7 days

14 days

0

0

0

5.2

0

7.5

15.6

5.0

10.0

52.0

5.0

20.00

155.9

10.0

37.5

519.3

7.5

32.5

1Nominal concentrations

Validity criteria fulfilled:
yes
Remarks:
Validity criteria of OECD Method 207 (1984) been fulfilled: The mortality in the controls does not exceed 10 per cent at the end of either test
Conclusions:
The 14-day LC50 of technical captan to earthworms was determined to be higher than 519.3 mg/kg.
Executive summary:

Test compound: Captan technical

 Protocol: OECD guidelines (1984) for testing of chemicals, entitled "Earthworm, Acute Toxicity Tests".

Test species: Earthworm, Eisenia foetida.

Duration of treatment: A Single soil treatment followed by a 14-day exposure period.

Treatments: Treatments included a negative control group and five groups treated with test compound at levels ranging from 5.2 mg/kg to 519.3 mg/kg .

Because of the limited number of mortalities in the test groups, it was not possible to determine LC50 values for Captan at 7 and 14 days. These values must lie in excess of the maximum dose level of 519.3 mg/kg.

The LC50 values for control group (chloroacetamide) were as follows:

 

Day 7:

LC50 value (ppm ai) 35.9 (95% confidence limits 20.6 – 68.9 ppm ai)

Day 14:

LC50 value (ppm ai) 20.8 (95% confidence limits 9.5 – 47.8 ppm ai)

 

The results indicate that Captan technical is of low toxicity to earthworms at concentrations of 5.2 to 519.3 mg/kg in soil.

Description of key information

Toxicity to soil macroorganisms except arthropods (Eisenia foetida): LC50 (14 day) >519,3 mg/kg for earthworms (Eisenia foetida), OECD Guideline No. 207 (1984) "Earthworm acute toxicity test"

Key value for chemical safety assessment

Short-term EC50 or LC50 for soil macroorganisms:
519.3 mg/kg soil dw

Additional information

The results indicate that captan is of low toxicity to earthworms at concentrations of 5.2 to 519.3 mg/Kg in soil. LC50 was not reached.