1-chloro-2-nitrobenzene

Genetic toxicity: in vivo

Administrative data

Endpoint:

in vivo mammalian germ cell study: gene mutation

Remarks:

Type of genotoxicity: gene mutation

Type of information:

experimental study

Adequacy of study:

other information

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Acceptable well documented report/publication meeting basic scientific principles.

Data source

Reference

Reference 1

Reference Type:

study report

Title:

Unnamed

Year:

1993

Reference 2

Reference Type:

publication

Title:

Unnamed

Year:

1985

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 477 (Genetic Toxicology: Sex-linked Recessive Lethal Test in Drosophila melanogaster)

Deviations:

yes

Principles of method if other than guideline:

Method: other: males(24 hrs old), mated with 3x with Basc virgin females brood1: 3d, brood2: 2d, brood3: 2d;

GLP compliance:

no

Type of assay:

Drosophila SLRL assay

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2-nitrobenzene

EC Number:

201-854-9

EC Name:

1-chloro-2-nitrobenzene

Cas Number:

88-73-3

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-2-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 2-Chloronitrobenzene
- Analytical purity: 99%
- Lot/batch No.: ET 00210KM
- Source: Aldrich Chemical Company (Milwaukee, WI)

Test animals

Species:

Drosophila melanogaster

Strain:

other: Canton-S wild type

Sex:

male

Details on test animals or test system and environmental conditions:

- Chemical Analyses by Midwest Research Institute (MRI; Kansas City, MO)
- Karl Fischer analysis indicated less than 0.3% water
- Test substance: purity: 99 %

Administration / exposure

Route of administration:

oral: feed

Vehicle:

5% sucrose

Duration of treatment / exposure:

72 hours

Doses / concentrations

Remarks:

Doses / Concentrations:
0, 125 ppm in 10 % ethanol and 5 % sucrose solution
Basis:

No. of animals per sex per dose:

no data

Positive control(s):

no data

Examinations

Evaluation criteria:

no data

Statistics:

Normal approximation to the binomial test according to Margolin et al., 1983

Results and discussion

Test results

Sex:

male

Genotoxicity:

negative

Toxicity:

yes

Vehicle controls validity:

valid

Negative controls validity:

not valid

Positive controls validity:

not valid

Additional information on results:

Toxicity tests were performed to set concentrations at a level that would induce 30% mortality after 72 hours of feeding

Any other information on results incl. tables

Induction of sex-linked recessive lethal mutations in Drosophila melanogaster by 2-Chloronitrobenzene

Route of exposure	Dose (ppm)	Incidence Deaths (%)	Incidence Sterility (%)	No. of Lethals / No. of X chromosomes tested			Total^1
				Mating 1	Mating 2	Mating 3
Study performed at; data published in Zimmering et al. (1989)
Larva feeding     Larva feeding	59 0   60 0	1     10	0     0	1/1349 2/1341   3/1309 1/1281	1/1344 0/1332   1/1326 0/1234		2/2693 (0.07%) 2/2673 (0.07%)   4/2635 (0.15%) 1/2515 (0.04%)
Study performed at; data published in Zimmering et al. (1985)
Feeding     Injection	125 0   10000 0	8     15	9     9	4/3481 1/2282   0/3065 1/1455	1/1759 0/785   0/2635 1/1345	0/1847 1/520   1/2450 4/1160	5/7087 (0.07%) 2/3587 (0.06%)   1/8150 (0.01%) 6/3960 (0.15%)

¹Total number of lethal mutations / total number of X chromosomes tested for up to three mating trials

Applicant's summary and conclusion

Conclusions:

Interpretation of results (migrated information): negative