1-chloro-2-nitrobenzene

Skin irritation / corrosion

Administrative data

Endpoint:

skin irritation: in vivo

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: see 'Remark'

Remarks:

Comparable to guideline study with acceptable restrictions. Considering both, the application site (inside of the rabbit ear as a thin and sensitve skin area) and the prolonged exposure duration (24 h) the reported absence of skin reactions appears sufficient for assessment.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 404 (Acute Dermal Irritation / Corrosion)

Deviations:

yes

Remarks:

only two animals used, application area inside of the animal ear, exposure duration 24 h instead of 4 h, no individual animal data (tabulation) given, lack of details on test substance

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2-nitrobenzene

EC Number:

201-854-9

EC Name:

1-chloro-2-nitrobenzene

Cas Number:

88-73-3

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-2-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 2-Chlor-1-nitrobenzol
- Analytical purity: no data

Test animals

Species:

rabbit

Strain:

other: albino

Details on test animals or test system and environmental conditions:

Animal
-Weight: 3,5-4 kg
-Source: Gierlich, Bochum, Germany
-Housing: single
-Diet: Höing-Kaninchenfutter 222, Höing, Verden/Aller, Germany

Test system

Type of coverage:

semiocclusive

Preparation of test site:

other: hairless inside of the ear cup

Vehicle:

unchanged (no vehicle)

Controls:

no

Amount / concentration applied:

500 mg

Duration of treatment / exposure:

24 hour(s)

Observation period:

7 days

Number of animals:

2

Details on study design:

TEST SITE
- Area of exposure: a 2 x2 cm area of the animal ear
- Cellulose pads were loaded with 500 mg 1 -chloro-2 -nitrobenzene.
- Type of wrap if used: The test areas were covered semi-occlusive with a layer of cellulose and plaster.

REMOVAL OF TEST SUBSTANCE
no data

SCORING SYSTEM:
no data

Results and discussion

Any other information on results incl. tables

No scoring data mentioned

Applicant's summary and conclusion

Interpretation of results:

not irritating

Remarks:

Migrated information no classification required. Criteria used for interpretation of results: EU

Executive summary:

Groening (Bayer AG), 1976

The skin irritation potential of 1 -chloro-2 -nitrobenzene was investigated in albino rabbits. A 2 x 2 cm area of the animal ear inside was used to applicate the test substance. Cellulose pads were loaded with 500 mg 1 -chloro-2 -nitrobenzene. The test areas were covered semi-occlusively with a layer of cellulose and plaster. The exposure duration was 24 hours.

No irritating effects were observed throughout the test and after 7 days of observation.