1-chloro-2-nitrobenzene

Acute Toxicity: oral

Administrative data

Endpoint:

acute toxicity: oral

Type of information:

experimental study

Adequacy of study:

key study

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 401 (Acute Oral Toxicity)

Deviations:

yes

Remarks:

: no pathology performed, lack of details on test substance

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2-nitrobenzene

EC Number:

201-854-9

EC Name:

1-chloro-2-nitrobenzene

Cas Number:

88-73-3

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-2-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 2-Chlor-1-nitrobenzol
- Physical state: solid
- Analytical purity: no data

Test animals

Species:

rat

Strain:

Wistar

Sex:

male/female

Details on test animals or test system and environmental conditions:

TEST ANIMALS
-Source: Winkelmann, Borchen, Germany
-Weight: 160-230g
-Housing: single per cage (Macrolon Type III)
-Diet: Altromin-Standarddiät, Altromin GmbH, Lage/Lippe, Germany

Administration / exposure

Route of administration:

oral: gavage

Vehicle:

polyethylene glycol

Details on oral exposure:

no data

Doses:

male: 50, 100, 150, 200, 250, 300, 500 mg/kg bw
female: 25, 50, 100, 250, 350, 500, 650, 850 mg/kg bw

No. of animals per sex per dose:

15

Control animals:

no

Details on study design:

Duration of observation period following administration: 14 days

Statistics:

LD50 was calculated by Probit-analysis (Fink and Hund 1965. Arzneim.-Forsch. 15:624), Litchfield and Wilcoxon (J. Pharmacol. Exp. Therap. 96, 99, 1949)

Results and discussion

Effect levels

Effect levels 1

Sex:

male

Dose descriptor:

LD50

Effect level:

219 mg/kg bw

95% CL:

196 - 243

Effect levels 2

Sex:

female

Dose descriptor:

LD50

Effect level:

457 mg/kg bw

95% CL:

398 - 518

Mortality:

Male rats:
150 mg/kg bw: 2/15 animals died 2d after application
200 mg/kg bw: 4/15 animals died 24h after application
250 mg/kg bw: 10/15 animals died between 1-2d after application
300 mg/kg bw: 14/15 animals died 24h after application
500 mg/kg bw: 15/15 animals died 24h after application

Female rats:
250 mg/kg bw: 1/15 animal died 8d after application
350 mg/kg bw: 2/15 animals died between 1-2d after application
500 mg/kg bw: 10/15 animals died between 1-2d after application
650 mg/kg bw: 12/15 animals died between 1-2d after application
850 mg/kg bw: 15/15 animals died between 1-2d after application

Clinical signs:

other: Reduced general condition and cyanotic appearance were observed in all male animals dosed 100 - 500 mg/kg bw and in all female rats dosed with 50 - 850 mg/kg bw

Gross pathology:

not examined

Any other information on results incl. tables

Acute oral toxicity

Dose (mg/kg)	Conc. %	Result	Signs of intoxication		Time of death
		m/s/n	Start	End
Male rats
50 100 150 200 250 300 500	1 2 3 4 5 6 10	0/0/15 0/15/15 2/15/15 4/15/15 10/15/15 14/15/15 15/15/15	- 49min 20min 16min 36min 13min 18min	- 5d 7d 7d 11d 9d -	- - 2d 24h 1-2d 24h 24h
Female rats
25 50 100 250 350 500 650 850	0.5 1 2 5 7 10 13 17	0/0/15 0/15/15 0/15/15 1/15/15 2/15/15 10/15/15 12/15/15 15/15/15	- 24h 24h 90min 11min 2h 8min 2h	- 3d 7d 7d 7d 13d 12d -	- - - 8d 1-2d 1-2d 1-2d 1-2d

m: number of rats which died

n: number of animals put in test

s: number of animals with signs of intoxication: reduced general condition, cyanotic appearance

Applicant's summary and conclusion

Conclusions:

DSD: Xn, R22 Harmful if swallowed
GHS: Acute Oral Category 3

Executive summary:

Gröning P (Bayer AG), 1976

The acute oral toxicity of 1 -chloro-2 -nitrobenzene was investigated in male and female Wistar II rats. 7 male and 8 female groups of 15 animals were dosed with 50, 100, 150, 200, 250, 300 and 500 mg/kg bw and 25, 50, 100, 250, 350, 500, 650 and 850 mg/kg bw 1 -chloro-2 -nitrobenzene per gavage and observed for 14 days following exposure for mortality and clinical signs.

Mortalities occurred at dose levels equal to and exceeding 150 mg/kg bw between 24 hours and 2 days after administration in male rats and in female rats at dose levels equal to and exceeding 250 mg/kg bw between 24 hours and 8 days. Reduced general condition and cyanotic appearance were observed in all male animals dosed 100 - 500 mg/kg bw and in all female rats dosed with 50 - 850 mg/kg bw. Symptoms started to appear 8 min (650 mg/kg bw, females) and 24 hours (50 and100 mg/kg bw, females) after administration, continuing up to 13 days. No clinical signs were observed in animals dosed with 25 mg/kg bw in females and 50 mg/kg bw in male rats. The calculated LD50 for male rats was 219 mg/kg bw and 457 mg/kg bw for female rats.