1-chloro-2-nitrobenzene

Acute Toxicity: dermal

Administrative data

Endpoint:

acute toxicity: dermal

Type of information:

experimental study

Adequacy of study:

other information

Study period:

1976

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions.

Data source

Reference

Reference Type:

study report

Title:

Unnamed

Year:

1976

Report date:

1976

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 402 (Acute Dermal Toxicity)

Deviations:

yes

Remarks:

: no pathology performed, lack of details on test substance

GLP compliance:

no

Test type:

standard acute method

Limit test:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2-nitrobenzene

EC Number:

201-854-9

EC Name:

1-chloro-2-nitrobenzene

Cas Number:

88-73-3

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-2-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 2-Chlor-1-nitrobenzol
- Physical state: solid
- Analytical purity: no data

Test animals

Species:

rat

Strain:

Wistar

Sex:

male

Administration / exposure

Type of coverage:

occlusive

Vehicle:

polyethylene glycol

Details on dermal exposure:

TEST SITE
- Area of exposure: shaved back
- Type of wrap if used: covered by alu and plaster


REMOVAL OF TEST SUBSTANCE
- Washing (if done): rinsed with water and soap
- Time after start of exposure: 24 hours

Duration of exposure:

24 hours

Doses:

male rats: 250, 350, 500, 750, 1000, 1500 mg/kg bw

No. of animals per sex per dose:

10

Control animals:

no

Details on study design:

- Duration of observation period following administration: 14 days

Results and discussion

Effect levels

Sex:

male

Dose descriptor:

LD50

Effect level:

655 mg/kg bw

Mortality:

See details below

Any other information on results incl. tables

dosis  conc.  result   signs of intoxication  time of death
mg/kg   %     m /s /n     start   end

 250    25    1/10/10     18 h   14 d             4 d
 350    25    1/10/10     18 h    7 d            18 h       
  
 500    50    3/10/10     18 h    9 d           1-3 d
 750    50    7/10/10     24 h   13 d           1-3 d 
1000    50    7/10/10     18 h    4 d        18 h - 3 d 
1500    75    9/10/10     18 h   14 d        18 h - 4 d

m:  number of rats which died; 
n: number of animals put in test
s: number of animals with signs of intoxication:
reduced general condition, difficulties in breathing,
cyanotic appearance, some animals showed lacrimation