1-chloro-2-nitrobenzene

Toxicity to aquatic algae and cyanobacteria

Administrative data

Endpoint:

toxicity to aquatic algae and cyanobacteria

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

other: Comparable to guideline study with acceptable restrictions. Adopted according to OECD SIDS. The original source is available and has been reviewed.

Data source

Reference

Reference 1

Reference Type:

publication

Title:

Quantitative Structure-Activity Relationships of Nitroaromatic Compounds to Four Aquatic Organisms.

Author:

Zhao Y-H, Yuan X, Ji G-D, Sheng L-X, Wang L-S

Year:

1997

Bibliographic source:

Chemosphere 34 (8): 1837 - 1844

Reference 2

Reference Type:

secondary source

Title:

1-Chloro-2-nitrobenzene CAS: 88-73-3, SIDS Initial Assessment Report

Author:

OECD

Year:

2001

Bibliographic source:

UNEP publications

Materials and methods

Test guideline

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 201 (Alga, Growth Inhibition Test)

Version / remarks:

(1984)

Deviations:

yes

Remarks:

: test duration 96 h instead of 72 h

GLP compliance:

no

Test material

Test material information

Constituent 1

Reference substance name:

1-chloro-2-nitrobenzene

EC Number:

201-854-9

EC Name:

1-chloro-2-nitrobenzene

Cas Number:

88-73-3

Molecular formula:

C6H4ClNO2

IUPAC Name:

1-chloro-2-nitrobenzene

Details on test material:

- Name of test material (as cited in study report): 2-Chloronitrobenzene
- Analytical purity: no data

Sampling and analysis

Analytical monitoring:

not specified

Test solutions

Vehicle:

yes

Details on test solutions:

- stock solutions were prepared in acteone

Test organisms

Test organisms (species):

other: algae: Scenedesmus obliquus

Study design

Test type:

not specified

Water media type:

freshwater

Limit test:

no

Total exposure duration:

96 h

Test conditions

Test temperature:

24 ± 1 °C

Nominal and measured concentrations:

- 5 concentrations
- 3 replicates

Details on test conditions:

TEST SYSTEM
- Type: closed
- Size, fill volume: 100 mL, 50 mL
- Initial cells density: 10E4 cells/mL
- No. of vessels per concentration (replicates): 3
- No. of vessels per control (replicates): 3


OTHER TEST CONDITIONS
- Photoperiod: 12 : 12 h
- Light intensity and quality: cool white fluorescent light, 4000 Lux (± 10%)


EFFECT PARAMETERS MEASURED:
- Determination of cell concentrations: spectrophotometer (at 650 nm)
- Observation intervals: daily

Reference substance (positive control):

no

Results and discussion

Effect concentrations

Duration:

96 h

Dose descriptor:

EC50

Effect conc.:

18.1 mg/L

Nominal / measured:

not specified

Conc. based on:

test mat.

Basis for effect:

growth rate

Applicant's summary and conclusion

Validity criteria fulfilled:

not specified

Executive summary:

Zhao (1997):

In a study similar to OECD 201 toxicity of 1-chloro-2-nitrobenzene to Scenedesmus obliquus was assessed. After 96 h of exposure an EC50 of 18.1 mg/L was determined based on growth rate.