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EC number: 246-680-4 | CAS number: 25155-30-0
Toxicity to terrestrial arthropods
Administrative data
Link to relevant study record(s)
- Endpoint:
- toxicity to terrestrial arthropods: short-term
- Type of information:
- other: published data
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted.
- GLP compliance:
- no
- Application method:
- soil
- Analytical monitoring:
- yes
- Details on sampling:
- Samples were taken 2, 17, and 31 days after the soil was spiked.
- Vehicle:
- not specified
- Details on preparation and application of test substrate:
- LAS was dissolved in water and mixed with 30 g moist soil (25.5 g dry). The mixture was prepared immediately before the experiment.
- Test organisms (species):
- Folsomia sp.
- Animal group:
- Collembola (soil-dwelling springtail)
- Details on test organisms:
- TEST ORGANISM
- Source: laboratory culture
- Age at test initiation (mean and range, SD): 23-26 days
- Weight at test initiation (mean and range, SD): - Study type:
- laboratory study
- Total exposure duration:
- 21 d
- Test temperature:
- 20 degrees C
- pH (if soil or dung study):
- 5.5
- Photoperiod and lighting:
- 12 hrs light/12 hrs dark
- Details on test conditions:
- TEST SYSTEM
- Amount of soil or substrate: 30 g moist
- No. of organisms per container (treatment): 10 male/10 female
- No. of replicates per treatment group: 4
- No. of replicates per control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Speyer, Germany
- Soil texture (if natural soil)
- % sand: 34.8
- % silt: 5.6
- % clay: 5.2
- % gravel: 54.6
- Organic carbon (%): 2.3
- Pretreatment of soil: dried at 80 degrees C
- Storage (condition, duration): 5 degrees C
OTHER TEST CONDITIONS
- Photoperiod: 12/12 hrs
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : survival and reproduction at 21 days of exposure measured using digital image processing - Nominal and measured concentrations:
- nominal: 0-1000 mg/kg dry soil
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 147 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: 116-178
- Details on results:
- Adult survival was unaffected at all concentrations. Reproductive output decreased linearly with increasing concentration, with 430 juveniles produced in control containers, to only 150 juveniles produced at the highest concentration (1000 mg/kg dw soil).
- Validity criteria fulfilled:
- yes
- Conclusions:
- The 21-day EC10 for F. fimetaria was 147 mg/kg dw soil based on reproduction.
- Executive summary:
Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted. There was no effect on adult survival, however, there was a dose-related decrease in reproduction. The EC10 based on reproduction was 147 mg/kg dw soil.
- Endpoint:
- toxicity to terrestrial arthropods: short-term
- Type of information:
- other: published data
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- The toxicity of LAS to soil dwelling organisms was tested in three soil types. Various LAS salts (Na, Ca, and Mg) were tested in one soil type (sandy) to determine the effection of speciation to toxicity. In each test, 10 male and 10 female organisms were placed in containers with test substance added to the soil. Exposure lasted 21 days, at the end of which the number of adult and juvenile organisms were counted.
- GLP compliance:
- no
- Application method:
- soil
- Analytical monitoring:
- yes
- Details on sampling:
- 30 g soil samples were stored at -20 degrees C until analysis
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- Acetone was used in the Ca-LAS and Mg-LAS tests as these compounds are insoluble in water.
- Test organisms (species):
- Folsomia sp.
- Animal group:
- Collembola (soil-dwelling springtail)
- Details on test organisms:
- TEST ORGANISM
- Source: laboratory culture
- Age at test initiation (mean and range, SD): 23-26 days
- Weight at test initiation (mean and range, SD): - Study type:
- laboratory study
- Total exposure duration:
- 21 d
- Test temperature:
- 20 degrees C
- pH (if soil or dung study):
- 5.5-5.8
- Photoperiod and lighting:
- 12:12 photoperiod
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): 5.5 x 6 cm cylindrical containers
- Amount of soil or substrate: 30 g moist soil
- No. of organisms per container (treatment): 10 male/10 female
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Lundgaard
- Soil texture (if natural soil)
- % sand: 66.9 coarse, 15.8 fine
- % silt: 8.6
- % clay: 6.2
- Soil classification system: sandy soil
- Organic carbon (%): 1.5
- CEC: 3.23 mEq/100 g
- Pretreatment of soil: dried at 80 degrees C, and sifted through 2 mm mesh
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Askov
- Soil texture (if natural soil)
- % sand: 38.4 coarse, 23.6 fine
- % silt: 22.3
- % clay: 13.0
- Soil classification system: loamy soil
- Organic carbon (%): 1.6
- CEC: 8.14 mEq/100 g
- Pretreatment of soil: dried at 80 degrees C, and sifted through 2 mm mesh
OTHER TEST CONDITIONS
- Photoperiod: 12:12
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: Flakkebjerg
- Soil texture (if natural soil)
- % sand: 23.4 coarse, 25.5 fine
- % silt: 34.2
- % clay: 15.1
- Soil classification system: clay soil
- Organic carbon (%): 1.0
- CEC: 10.16 mEq/100 g
- Pretreatment of soil: dried at 80 degrees C, and sifted through 2 mm mesh
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : number of surviving adults and juveniles counted on day 21 - Nominal and measured concentrations:
- 0, 8, 28, 79, 278, 793 mg/kg dry weigth measured
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 85 mg/kg soil dw
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Na-LAS in sandy soil
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 93 mg/kg soil dw
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Na-LAS in clay soil
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 87 mg/kg soil dw
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Na-LAS in sandy soil
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 106 mg/kg soil dw
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Ca-LAS in sandy soil
- Duration:
- 21 d
- Dose descriptor:
- EC10
- Effect conc.:
- 97 mg/kg soil dw
- Nominal / measured:
- meas. (not specified)
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks on result:
- other: Mg-LAS in sandy soil
- Details on results:
- Soil type had little effect on the toxicity of Na-LAS to soil organisms. Salt speciation also had little effect on toxicity.
- Validity criteria fulfilled:
- yes
- Conclusions:
- The EC10 for Folsomia fimetaria in various soils ranged from 85-93 mg/kg dry weight soil.
- Executive summary:
Groups of 10 male and 10 female Folsomia fimetaria were exposed to various concentrations of Na-LAS in three different soil types for 21 days. At the end of this period, the number of adult and juvenile organisms was counted. Another test was done to compare the toxicity of three LAS salts, Na, Ca, and Mg, in one soil type (sandy). There was little variation in toxicity among the three soil types and in the three salt species. The EC10 for Folsomia fimetaria in various soils ranged from 85-93 mg/kg dry weight soil
Referenceopen allclose all
Description of key information
Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted. There was no effect on adult survival, however, there was a dose-related decrease in reproduction.
The EC10 based on reproduction was 147 mg/kg dw soil.
Groups of 10 male and 10 female Folsomia fimetaria were exposed to various concentrations of Na-LAS in three different soil types for 21 days. At the end of this period, the number of adult and juvenile organisms was counted. Another test was done to compare the toxicity of three LAS salts, Na, Ca, and Mg, in one soil type (sandy). There was little variation in toxicity among the three soil types and in the three salt species.
The EC10 for Folsomia fimetaria in various soils ranged from 85-93 mg/kg dry weight soil
Key value for chemical safety assessment
- Short-term EC50 or LC50 for soil dwelling arthropods:
- 425 mg/kg soil dw
- Long-term EC10, LC10 or NOEC for soil dwelling arthropods:
- 85 mg/kg soil dw
Additional information
Groups of 10 male and 10 female F. fimetaria were exposed to concentrations of LAS ranging from 0 -1000 mg/kg dw soil for 21 days. At the end of the 21 days, the number of F. fimetaria were counted, and the number of surviving adults and juveniles as the result of reproduction were counted. There was no effect on adult survival, however, there was a dose-related decrease in reproduction.
The EC10 based on reproduction was 147 mg/kg dw soil.
Groups of 10 male and 10 female Folsomia fimetaria were exposed to various concentrations of Na-LAS in three different soil types for 21 days. At the end of this period, the number of adult and juvenile organisms was counted. Another test was done to compare the toxicity of three LAS salts, Na, Ca, and Mg, in one soil type (sandy). There was little variation in toxicity among the three soil types and in the three salt species.
The EC10 for Folsomia fimetaria in various soils ranged from 85-93 mg/kg dry weight soil
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