Sodium dodecylbenzenesulfonate

Biodegradation in water: screening tests

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Justification for type of information:

Sodium dodecylbenzenesulfonate is one of the linear alkylbenzene sulfonic acids (LAS). Sodium dodecylbenzenesulfonate is regarded with 12C-LAS.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 F (Ready Biodegradability: Manometric Respirometry Test)

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Details on inoculum:

The activated sludge sample was obtained from the pilot-plant during period A in this study.

Duration of test (contact time):

28 d

Initial conc.:

25.1 mg/L

Based on:

test mat.

Details on study design:

This test was carried out according to the guidelines of OECD test method encoded 301F. An automated sapromate(Voith) was used to follow O2 consumption in response to LAS mineralisation. A test with a blank inoculm was included as well as a test with 109mg/l of the ezsily biodegradable potassium hydrogen phthalate (PHP) to serve as a method control. All the tests were carried out in duplicate over a period of 28 days at a temperature of 20 deg C.

Reference substance:

other: potassium hydrogen phthalate (PHP)

Parameter:

other: mineralisation

Value:

> 60

Remarks on result:

other: within 28days

Details on results:

The O2-consumption data generated in the ready biodegradability tests(data not shown) showed that LAS is readily biodegradable as defined by the OECD Guidelines: more than 60% of mineralisation was achieved within 28 days and this level was reached within a time windows of 10 days from the start of mineralisation. From the O2-consumption data a biomass-yoeld Y of 0.50 (+/-) 0.02 mg^-1 O2 and a maximum specific growth rate of 0.050 (+/-) 0.003 h^-1 were estimated.

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Data of following studies are demonstrating rapid biodegradation of C12- LAS (including Na-LAS) under aerobic and anaerobic conditions. The result is that more than 60% of mineralisation was achieved within 28 days.

Executive summary:

C12-LAS is readily biodegradable (more than 60% by mineralization wihin 28days) [OECD TG 301 F].

Reference 2

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

equivalent or similar to guideline

Guideline:

OECD Guideline 301 E (Ready biodegradability: Modified OECD Screening Test)

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

natural water

Details on inoculum:

To each liter of sterile Bay water was added 1.0 mL of sterile BOD buffer and 5 mL of a “seed” culture. The bacterial “seed” consisted of the sample of (non-filtered) Bay water from May 30, 1972 which was supplemented with DBS (10 ppm) and incubated at 20 deg C for 17 days, by which time more than 75% degradation had occurred.

Duration of test (contact time):

17 d

Initial conc.:

10 other: ppm

Based on:

test mat.

Details on study design:

Detergent decomposition depends on aerobic respiratory processes, incubation in a closed vessel results in depletion of dissolved oxygen. To determine oxygen utilization during detergent oxidation a BOD-type test was employed with sodium dodecylbenzene sulfonate as substrate. Bay water aged more than 30 days was sterilized by membrane filtration (Millipore, 0.45 um).

Parameter:

% degradation (test mat. analysis)

Value:

> 75

Sampling time:

11 d

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Biodegradation of sodium dodecylbenzene sulfonate (DBS), at 10 ppm was measured 75% after 11 days and test temperature was maintained at 20 deg C for 17 days. The sodium dodecylbenzene sulfonate (DBS) could be conclued as readily degradable.

Reference 3

Endpoint:

biodegradation in water: ready biodegradability

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

data from handbook or collection of data

Justification for type of information:

Dodecylbenzene sulfonic acids (CAS# 27176-87-0 , EC Number; 248-289-4) ) is a very close analogue of Sodium dodecylbenzenesulfonate (CAS No. 25155-30-0, EC Number; 246-680-4) ) and the dissociated acid it readily dissociates in water and release the dodecylbenzene sulfonic anion in solution.

Qualifier:

according to guideline

Guideline:

OECD Guideline 301 B (Ready Biodegradability: CO2 Evolution Test)

GLP compliance:

not specified

Oxygen conditions:

aerobic

Inoculum or test system:

activated sludge (adaptation not specified)

Duration of test (contact time):

28 d

Initial conc.:

10 mg/L

Based on:

DOC

Initial conc.:

20 mg/L

Based on:

DOC

Details on study design:

This test was carried out according to the guidelines of OECD Guide-line 301 B "Ready Biodegradability: Modified Sturm Test (CO2 evolution)"
% degradation refers to mineralization. . All the tests were carried out in duplicate over a period of 28 days at a temperature of 20 deg C.

Reference substance:

not specified

Parameter:

other: mineralisation

Value:

69

Remarks on result:

other: after 28 day

Details on results:

Data of following studies are demonstrating rapid biodegradation of Benzene sulfonic acid, dodecyl- (27176-87-0) under aerobic conditions.
The result is that it was achieved 69% mineralization after 28 days.This test was carried out according to the guidelines of OECD Guide-line 301 B "Ready Biodegradability: Modified Sturm Test (CO2 evolution)"

Validity criteria fulfilled:

yes

Interpretation of results:

readily biodegradable

Conclusions:

Data of following studies are demonstrating rapid biodegradation of Benzene sulfonic acid, dodecyl- (27176-87-0) under aerobic conditions.
The result is that it was achieved 69% mineralization after 28 days.This test was carried out according to the guidelines of OECD Guide-line 301 B "Ready Biodegradability: Modified Sturm Test (CO2 evolution)"

Executive summary:

Data of following studies are demonstrating rapid biodegradation of Benzene sulfonic acid, dodecyl- (27176-87-0) under aerobic conditions. The result is that it was achieved 69% mineralization after 28 days.This test was carried out according to the guidelines of OECD Guide-line 301 B "Ready Biodegradability: Modified Sturm Test (CO2 evolution)"

Description of key information

Biodegradation in water:screening tests
 
Data of following studies are demonstrating rapid biodegradation of C12-LAS (including sodium dodecylbenzene sulfonate (DBS)) under aerobic conditions. Temmink and Klapwijk (2004) conducted OECD 301F test and the result is that more than 60% of mineralisation was achieved within 28 days.
Cook and Glodman, 1974 conducted Biodegradation of sodium dodecylbenzene sulfonate (DBS), at 10 ppm was measured 75% after 11 days and test temperature was maintained at 20 deg C for 17 days. The sodium dodecylbenzene sulfonate (DBS) could be conclued as readily degradable.
For dodecylbenzene sulfonic acid, the available study indicates 69% of the material mineralized after 28 days ,OECD 301B test (USEPA 1992).

Key value for chemical safety assessment

Biodegradation in water:

readily biodegradable

Additional information

Biodegradation in water:screening tests

 

Data of following studies are demonstrating rapid biodegradation of C12-LAS (including sodium dodecylbenzene sulfonate (DBS)) under aerobic conditions. Temmink and Klapwijk (2004) conducted OECD 301F test and the result is that more than 60% of mineralisation was achieved within 28 days.

Cook and Glodman, 1974 conducted Biodegradation of sodium dodecylbenzene sulfonate (DBS), at 10 ppm was measured 75% after 11 days and test temperature was maintained at 20 deg C for 17 days. The sodium dodecylbenzene sulfonate (DBS) could be conclued as readily degradable.

For dodecylbenzene sulfonic acid, the available study indicates 69% of the material mineralized after 28 days ,OECD 301B test (USEPA 1992).

Table     Summary of Biodegradation in water:screening tests

 

Type/ Method	Test material	Degradation	Duration	References
Aerobic/ OECD 301F	C12-LAS	> 60 % of mineralisation	28 days	Temmink and Klapwijk, 2004
Aerobic/ Other	Sodium dodecyl benzenesulfonate	75 %	11 days	Cook and Glodman, 1974
Aerobic/ OECD 301B	Dodecylbenzenesulfonic acid	69 % of mineralisation	28 days	USEPA 1992