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EC number: 246-680-4 | CAS number: 25155-30-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
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- Flash point
- Auto flammability
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- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
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- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
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- Endpoint summary
- Stability
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- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
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- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
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- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
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- Additional toxicological data

Genetic toxicity: in vitro
Administrative data
- Endpoint:
- in vitro gene mutation study in bacteria
- Remarks:
- Type of genotoxicity: gene mutation
- Type of information:
- other: published data
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- comparable to guideline study
- Justification for type of information:
- Dodecylbenzene sulfonic acids (CAS# 27176-87-0 , EC Number; 248-289-4) ) is a very close analogue of Sodium dodecylbenzenesulfonate (CAS No. 25155-30-0, EC Number; 246-680-4) ) and the dissociated acid it readily dissociates in water and release the dodecylbenzene sulfonic anion in solution.
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 008
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 471 (Bacterial Reverse Mutation Assay)
- GLP compliance:
- yes
- Type of assay:
- bacterial reverse mutation assay
Test material
- Reference substance name:
- Dodecylbenzenesulphonic acid
- EC Number:
- 248-289-4
- EC Name:
- Dodecylbenzenesulphonic acid
- Cas Number:
- 27176-87-0
- Molecular formula:
- C18H30O3S
- IUPAC Name:
- Dodecylbenzenesulfonic acid
- Test material form:
- other: liquid
- Details on test material:
- - KIT Code No.: K-2698- Supplier: Korea Chemicals Management Association (KCMA)- Lot No.: 080108C105- Purity: 96.87%- Molecular Weight: 326.54- Density: 1.0550 at 20 deg C- Date of Receipt: January 14, 2008- Quantities received: 3 kg- Appearance: Liquid- Life time: January 8, 2009- Storage Conditions: Room Temperature [Archives No.: KIT-401(106)]
Constituent 1
Method
Species / strainopen allclose all
- Species / strain / cell type:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Species / strain / cell type:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Metabolic activation system:
- - Species and cell type: Rat (Sprague Dawley strain), male, liver - Quantity: 5 % S9 mix induced with Aroclor 1254
- Test concentrations with justification for top dose:
- - TA 98: 23.44, 46.88, 93.75, 187.5, 375, 750, 1,500 μg/plate (-)
- TA 98: 5.86, 11.72, 23.44, 46.88, 93.75, 187.5, 375, 750, 1,500, 3,000 μg/plate (+)
- TA 100: 5.86, 11.72, 23.44, 46.88, 93.75, 187.5 μg/plate (-)
- TA 100: 23.44, 46.88, 93.75, 187.5, 375, 750 μg/plate (+)
- TA 1535: 5.86, 11.72, 23.44, 46.88, 93.75, 187.5 μg/plate (-)
- TA 1535: 23.44, 46.88, 93.75, 187.5, 375, 750 μg/plate (+)
- TA 1537: 5.86, 11.72, 23.44, 46.88, 93.75, 187.5 μg/plate (-)
- TA 1537: 23.44, 46.88, 93.75, 187.5, 375, 750 μg/plate (+)
- WP2uvrA: 5.86, 11.72, 23.44, 46.88, 93.75, 187.5 μg/plate (-)
- WP2uvrA: 23.44, 46.88, 93.75, 187.5, 375, 750, 1500 μg/plate (+) - Vehicle / solvent:
- Dimethylsulfoxide
Controls
- Untreated negative controls:
- yes
- Remarks:
- Dimethylsulfoxide
- Negative solvent / vehicle controls:
- yes
- Remarks:
- Dimethylsulfoxide
- Positive controls:
- yes
- Positive control substance:
- other: 2-Nitrofluorene, Sodium azide, 9-Aminoacridine, 4-Nitroquinoline 1-oxide ,2-Aminoanthracene and Benzo(a)pyrene
- Details on test system and experimental conditions:
- - Number of replicated: 3 plates/dose - Dose range finding experiment was carried out using dose levels of 8, 40, 200, 1,000, 3,000 and 5,000 μg/plate both in the absence and in the presence of metabolic activation system.- Cytotoxicity: A preliminary toxicity test was performed to define the concentrations to be used for the mutagenicity study.
- Evaluation criteria:
- The assay was considered valid if the number of spontaneous revertant colonies in vehicle control plates falls within the normal range and the positive control chemicals induce significant increases in the number of mutagen-induced revertant colonies compared to vehicle control. It was judged to be positive if there was a concentration-related increase over the range tested and/or a reproducible increase at one or more concentrations in the number of revertant colonies per plate in at least one strain with or without the metabolic system. In addition, it was judged to have a toxic effect (antibacterial effect) when a clearing or diminution of background lawn, the appearance of micro-colonies, and/or thedecrease more than 50% in the number of colonies compared to that of vehicle control was observed.
Results and discussion
Test resultsopen allclose all
- Species / strain:
- S. typhimurium TA 1535, TA 1537, TA 98 and TA 100
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- ≥375 μg/plate (+) (TA 100, TA 1535, TA 1537) ≥93.75 μg/plate (-) (TA 100, TA 1535, TA 1537) ≥1500 μg/plate (+) (TA 98) ≥375 μg/plate (-) (TA 98)
- Species / strain:
- E. coli WP2 uvr A
- Metabolic activation:
- with and without
- Genotoxicity:
- negative
- Cytotoxicity / choice of top concentrations:
- cytotoxicity
- Remarks:
- ≥750 μg/plate (+) (WP2 uvr ) 1500 μg/plate (+) (WP2 uvr )
- Remarks on result:
- other: strain/cell type: TA 98, 100, 1535, 1537 and WP2 uvr A
- Remarks:
- Migrated from field 'Test system'.
Any other information on results incl. tables
Bacterial tests
A bacterial reverse mutation test was performed in accordance with [OECD TG 471] using Salmonella typhimurium (strains TA98,TA100, TA1535 and TA 1537) and Escherichia coli (strain WP2uvrA) with and without a metabolic activation system. Test doses were as follows:
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5.86, 11.72, 23.44, 46.88, 93.75, 187.5, 375, 750, 1,500, 3,000μg/plate (+/-) |
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Dimethylsulfoxide (DMSO) was used as a vehicle and Sodium azide (SA), 2-Nitrofluorene (2-NF), 9-Aminoacridine (9-AA), 4-Nitroquinoline 1-oxide (4NQO),2-Aminoanthracene (2-AA)andBenzo(a)pyrene (BP)were used as positive control item.In all strains used, there was no increase in the number of revertant colonies compared to the vehicle control at any concentration of dodecylbenzenesulfonic acid either in the presence or absence of metabolic activation system. However, in the first and second experiment, it was observed a clearing or diminution of background lawn, the appearance of micro-colonies, and/or more than 50% decrease in the number of colonies at more than 375 μg/plate and 93.75 μg/plate in TA100, TA1535 and TA1537 strains in the presence and absence of S9 activation, respectively. Also, in TA98 strain, it was observed a clearing or diminution of background lawn, the appearance of micro-colonies, and/or more than 50% decrease in the number of colonies at more than 1500 μg/plate and 375μg/plate in the presence and absence of S9 activation, respectively. InE. coliWP2uvrA strain, the more than 50% decrease in the number of colonies was observed at more than 750μg/plate and at 1500 μg/plate in the presence of S9 activation in the first and second experiment, respectively. The positive controls induced a significant increase in the numbers of revertant colonies indicating the assay was valid.
Applicant's summary and conclusion
- Conclusions:
- Interpretation of results :negative
In the presence and absence of metabolic system, no mutation in the Salmonella typhimurium (strains TA 98, TA 100, TA 1535 and TA 1537) and Escherichia coli (strain WP2 uvrA) occurred with dodecylbenzenesulfonic acid (as a read across for Sodium dodecylbenzenesulfonate) .
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