Sodium dodecylbenzenesulfonate

Toxicity to other aquatic organisms

Link to relevant study record(s)

Reference

Reference 1

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

Thirty mussels (5.8 cm average main axis length) collected from a mussel cultivation area of the Lagoon of Venice, Italy were divided into groups of 10 and placed in net tubes in a 60-L tank for 7 days in contact with 50 mg/L continuously suspended LAS-spiked sediments. Mussels were fed an algal suspension and water, food, and sediment were renewed daily. Filtration rate was determined twice a day (immediately before and after water changes) as defined by the volume of water cleared of algal particles/animal/hour. Faeces were collected daily and pooled for analysis of LAS concentrations by HPLC. Oxygen consumption and ammonia excretion rates were determined at the end of each treatment.

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

no

Test organisms (species):

other: Mytilus galloprovincialis

Details on test organisms:

Mytilus galloprovincialis (marine mussel).
Thirty mussels (5.8 cm average main axis length) collected from a mussel cultivation area of the Lagoon of Venice, Italy

Test type:

semi-static

Water media type:

saltwater

Limit test:

yes

Total exposure duration:

7 d

Nominal and measured concentrations:

50 mg/L continuously suspended LAS-spiked sediments

Details on test conditions:

Mussels were divided into groups of 10 and placed in net tubes in a 60-L tank for 7 days in contact with 50 mg/L continuously suspended LAS-spiked sediments. Mussels were fed an algal suspension and water, food, and sediment were renewed daily.

Reference substance (positive control):

not specified

Duration:

7 d

Dose descriptor:

NOEC

Effect conc.:

32.19 other: mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: filtration rate, oxygen uptake, nitrogen excretion

Details on results:

No significant differences in survival or physiological responses between treatments and controls were observed.
NOEC = 32.19 mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)

The LAS concentration in treated sediments decreased by about 90% over the duration of the study (mean 132 mg/kg at initiation to mean 7.85 mg/kg at completion).

Validity criteria fulfilled:

yes

Conclusions:

NOEC = 32.19 mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)

Reference 2

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

other: published data

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

study well documented, meets generally accepted scientific principles, acceptable for assessment

Qualifier:

no guideline followed

Principles of method if other than guideline:

Tests were conducted as an aqueous fraction in the presence of sediment. Natural stream sediments (71% clay, 19% fine silt, 4% medium sand, 6% fine sand) were collected from a pristine site in Rapid Creek, SD. Before testing, wet sediment was autoclaved for 40-60 minutes to reduce microbial populations and minimize initial rates of surfactant biodegradation. LAS was added to a sediment slurry at a nominal concentration and stirred overnight, then 350 g was poured into each test chamber and allowed to settle. The organic carbon content of the test sediment was 4.2% prior to testing. A flow-through diluter system delivered test material in water to glass containers with 120-140 cm2 bottom surface area each. Test concentrations were control, 8, 42, 146, 319, and 993 ppm. Intact egg masses were incubated in Petri dishes containing 20-30 mL of dilution water at 22 °C until hatching commenced. Newly hatched larvae were allowed to mature 72 hours before testing. Twenty larvae were randomly distributed to each duplicate test chamber for each of five test concentrations plus the controls. Larvae were fed daily until emergence of the first adult in each chamber. Tests were continued until each midge emerged as an adult or larvae were determined to be dead. The number of winged adults was recorded daily. The average test duration was 24 days. Total hardness, pH, dissolved oxygen, and temperature were monitored frequently during the test.

GLP compliance:

no

Analytical monitoring:

yes

Vehicle:

not specified

Details on test solutions:

Natural stream sediments (71% clay, 19% fine silt, 4% medium sand, 6% fine sand) were collected from a pristine site in Rapid Creek, SD. Before testing, wet sediment was autoclaved for 40-60 minutes to reduce microbial populations and minimize initial rates of surfactant biodegradation.

Test organisms (species):

other: Chironomus riparius

Details on test organisms:

Chironomus riparius (Insecta, Midge)

Test type:

flow-through

Water media type:

not specified

Limit test:

no

Total exposure duration:

24 h

Post exposure observation period:

closed-system

Nominal and measured concentrations:

Test concentrations were control, 8, 42, 146, 319, and 993 ppm.

Details on test conditions:

LAS was added to a sediment slurry at a nominal concentration and stirred overnight, then 350 g was poured into each test chamber and allowed to settle. The organic carbon content of the test sediment was 4.2% prior to testing. A flow-through diluter system delivered test material in water to glass containers with 120-140 cm² bottom surface area each. Intact egg masses were incubated in Petri dishes containing 20-30 mL of dilution water at 22 °C until hatching commenced. Newly hatched larvae were allowed to mature 72 hours before testing. Twenty larvae were randomly distributed to each duplicate test chamber for each of five test concentrations plus the controls. Larvae were fed daily until emergence of the first adult in each chamber. Tests were continued until each midge emerged as an adult or larvae were determined to be dead.

Reference substance (positive control):

yes

Duration:

24 h

Dose descriptor:

NOEC

Effect conc.:

319 other: ppm in sediment

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: emergence

Duration:

24 h

Dose descriptor:

LOEC

Effect conc.:

993 other: ppm in sediment

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: emergence

Details on results:

Adults typically emerged 12-14 days after hatching. Control values for adult emergence were similar to or exceeded the historical average observed in their laboratory (>90%). Percent emergence was 98, 95, 90, 90, 90, and 73 for the control, 8, 42, 146, 319, and 993 ppm concentrations, respectively. For comparison, additional flow-through studies were conducted without sediment.

Results indicate that sorption onto sediment significantly mitigates LAS bioavailability.

Validity criteria fulfilled:

yes

Conclusions:

The NOEC for Chironomus riparius in sediment was 319 ppm, and the LOEC was 993 ppm based on emergence.

Reference 3

Endpoint:

toxicity to other aquatic vertebrates

Type of information:

experimental study

Adequacy of study:

key study

Reliability:

2 (reliable with restrictions)

Rationale for reliability incl. deficiencies:

comparable to guideline study with acceptable restrictions

Qualifier:

no guideline followed

Principles of method if other than guideline:

An integrated model stream ecosystem fate and effects study of a C12LAS homologue, with a high content (35.7%) of its most hydrophobic and toxic 2-phenyl isomer, was performed in the summer and fall of 1996 in Procter and Gamble’s Experimental Stream facility. The study addressed responses of periphytic microbes, immature benthic fauna including abundance and drift, and emergence of adult insects in a 56-day exposure.

GLP compliance:

not specified

Analytical monitoring:

yes

Details on sampling:

Sampling was done two to three times weekly at both the head and tail of each stream. Sampling was done in triplicate.

Vehicle:

no

Test organisms (species):

other: mayfly, chironomid, and aquatic worm

Details on test organisms:

other: Baetis sp. (mayfly), Isonychia sp. (mayfly), Stenonema sp. (mayfly), Thienemannimyla sp. (chironomid), Tanytarsus sp. (chironomid), Cricotopus sp. (chironomid), Polypedilum sp. (chironomid), Reotanytarsus sp. (chironomid), Naididae (aquatic worm)

Test type:

other: out-door experimental streams

Water media type:

freshwater

Limit test:

no

Total exposure duration:

56 d

Remarks on exposure duration:

Exposures ranged from 126 to 2978 μg/L

Details on test conditions:

An integrated model stream ecosystem fate and effects study of a C12LAS homologue, with a high content (35.7%) of its most hydrophobic and toxic 2-phenyl isomer, was performed in the summer and fall of 1996 in Procter and Gamble¿s Experimental Stream facility. The study addressed responses of periphytic microbes, immature benthic fauna including abundance and drift, and emergence of adult insects in a 56-day exposure.

Duration:

56 d

Dose descriptor:

NOEC

Effect conc.:

268 µg/L

Nominal / measured:

nominal

Conc. based on:

test mat.

Basis for effect:

other: bioavailailability model

Details on results:

Microbial heterotrophs acclimated to C12LAS exposure quickly (14 days) and biodegraded C12LAS at all concentrations. Blue-green algae responded by increasing in abundance with increasing C12LAS concentration. Invertebrates responded by increased drift and reduced benthic abundances at concentrations exceeding 293 μg/LL. Emergence at 927 ¿g/L also declined relative to the control. Adverse responses for mayflies and chironomids were indicated using univariant statistical techniques. Multivariant techniques indicated these taxa plus molluscs, aquatic worms, caddisflies, and stoneflies were impaired at some concentrations. Bioavailability of C12LAS was investigated in streams as a function of the total suspended solids load in the water column driven by local weather and watershed patterns. A continuous bioavailability model indicated exposure was reduced by an average of 8.5 ± 8.9%. A model ecosystem NOEC (no-observed-effect-concentration) was concluded to be 293 ¿g/L based on measured water column exposure and adjusted to 268 μg/LL by the bioavailability model. A summary of selected population and community responses at 8 weeks from the current study is shown in the table below.

Community/Measure	Dose Response	Temporal	NOEC (µg/L)
Heterotrophic microbial Biomass (total lipid phosphate/mm^2) Amino acid uptake (^3H dpm/mm^2/min) Phospholipid fatty acid (PLFA) distr. Surfactant mineralization (% CO2)	NS NS	Shift at >293 µg/L Acclimation at all conc.
Autotrophic microbial Bicarbonate uptake (^14C dpm/mm^2/min) Algal density (cells/mm^2) Algal biovolume (µm^3/mm^2) Blue-green algal density (cells/mm^2) Green algal density (cells/mm^2) Diatom algal density (cells/mm^2) Algal richness Dominant taxa (cells/mm^2)   Cocconeis placentula   Melosira varians   Chrococcus sp.   Nitzschia dissipata   Navicula salinarum v. intermedia   Pleurosira (= Biddulphia) laevis   Nitzschia inconspicua   Nitzschia palea   Diatoma vulgare   Gyrosigma acuminatum	+ NS NS ++ NS NS - - NS + NS NS NS ++ + -- -	+ ++ - - + ++ + -- -	927 927 927 927
Invertebrates Richness Diversity (Shannon-Weaver) Total abundance (No./m^2) Insect abundance (No./m^2) EPT abundance (No./m^2) Mayfly abundance (No./m^2) Caddisfly abundance (No./m^2) True fly abundance (No./m^2) Chironomid abundance (No./m^2) Mollusk abundance (No./m^2) Oligochaete abundance (No./m^2) Dominant populations (No./m^2)   Baetis sp. (mayfly)   Isonychia sp. (mayfly)   Stenonema sp. (mayfly)   Thienemannimyla sp. (chironomid)   Tanytarsus sp. (chironomid)   Cricotopus sp. (chironomid)   Polypedilum sp. (chironomid)   Reotanytarsus sp. (chironomid)   Naididae (aquatic worm)	NS NS -- NS - NS NS NS NS NS -- b --- NS --- ++ + + NS NS	-- - -- --- --- ++ + +	293 927 293 927 293

a Plus (+) and minus (-) signs indicate whether the response significantly increased or decreased from the control condition (± = 0.05). The strength to the response was graded as slight (+/-), moderate (++/--), or great (+++/---) based on statistical analyses.   NS indicates not significant. ^bTaxon too low in abundance, emerged.

 

Validity criteria fulfilled:

yes

Conclusions:

A model ecosystem NOEC (no-observed-effect-concentration) was concluded to be 293 μg/L based on measured water column exposure and adjusted to 268 μg/Lby the bioavailability model.

Executive summary:

An integrated model stream ecosystem fate and effects study of a C12LAS homologue, with a high content (35.7%) of its most hydrophobic and toxic 2-phenyl isomer, was performed in the summer and fall of 1996 in Procter and Gambles Experimental Stream facility. The study addressed responses of periphytic microbes, immature benthic fauna including abundance and drift, and emergence of adult insects in a 56-day exposure. A model ecosystem NOEC (no-observed-effect-concentration) was concluded to be 293 microgram/L based on measured water column exposure and adjusted to 268 microgram/L by the bioavailability model.

Description of key information

No significant differences in survival or physiological responses between treatments and controls were observed in the study of Marin, M.G., Pivotti, L., Campesan, G., Turchetto, M. and Tallandini, L.1994.
Thirty mussels (5.8 cm average main axis length) collected from a mussel cultivation area of the Lagoon of Venice, Italy were divided into groups of 10 and placed in net tubes in a 60-L tank for 7 days in contact with 50 mg/L continuously suspended LAS-spiked sediments. Mussels were fed an algal suspension and water, food, and sediment were renewed daily. Filtration rate was determined twice a day (immediately before and after water changes) as defined by the volume of water cleared of algal particles/animal/hour. Faeces were collected daily and pooled for analysis of LAS concentrations by HPLC. Oxygen consumption and ammonia excretion rates were determined at the end of each treatment.
NOEC = 32.19 mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)

Additional information

No significant differences in survival or physiological responses between treatments and controls were observed in the study of Marin, M.G., Pivotti, L., Campesan, G., Turchetto, M. and Tallandini, L.1994.

Thirty mussels (5.8 cm average main axis length) collected from a mussel cultivation area of the Lagoon of Venice, Italy were divided into groups of 10 and placed in net tubes in a 60-L tank for 7 days in contact with 50 mg/L continuously suspended LAS-spiked sediments. Mussels were fed an algal suspension and water, food, and sediment were renewed daily. Filtration rate was determined twice a day (immediately before and after water changes) as defined by the volume of water cleared of algal particles/animal/hour. Faeces were collected daily and pooled for analysis of LAS concentrations by HPLC. Oxygen consumption and ammonia excretion rates were determined at the end of each treatment.

NOEC = 32.19 mg/kg dry weight (geometric mean of initial [132 mg/L] and final [7.85 mg/L] LAS concentration)