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Short-term toxicity to aquatic invertebrates

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Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
(Q)SAR
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to guideline
Guideline:
other: QSAR model
Principles of method if other than guideline:
EPI Suite v 4.0/ECOSARv1.00
GLP compliance:
no
Remarks:
not applicable. QSAR model
Analytical monitoring:
not required
Vehicle:
no
Test organisms (species):
other: Daphnid species
Test type:
other: QSAR model
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
not applicable. QSAR model
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Dissolved oxygen:
not applicable. QSAR model
Salinity:
not applicable. QSAR model
Nominal and measured concentrations:
not applicable. QSAR model
Details on test conditions:
100% Benzenesulfonic acid, dodecyl-, sodium salt
SMILES : O([Na])S(=O)(=O)c1ccc(cc1)CCCCCCCCCCCC
CHEM : Benzenesulfonic acid, dodecyl-, sodium salt
CAS Num: 25155-30-0
MOL FOR: C18 H29 O3 S1 Na1
MOL WT : 348.48
Log Kow: 3.00 (KowWin estimate)
Wat Sol: 800 mg/L (experimental database)
Reference substance (positive control):
not required
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
23.265 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.

Overview of QSARs estimations on short-term aquatic toxicity of

Sodium dodecylbenzenesulfonate/Benzenesulfonic acid, dodecyl-, sodium salt

to Daphnid     

 

Method

 

Results

Remarks

Reference

QSAR

estimate

Daphnid , 48-hr LC50 =23.265 mg/L (ppm)

 

Daphnid, 30-day, ChV (chronic value) = 3.159 mg/L (ppm)

 

 

Reliable with restrictions,

QSAR estimated data

ECOSAR Program (v1.00)

 

Using the EPIWIN ECOSAR QSAR model aLC50 for fresh water Daphnid of 23.265 mg/l was established. 

Daphnid , 48-hr LC50 =23.265 mg/L (ppm)

Daphnid, 30-day, ChV (chronic value) = 3.159 mg/L (ppm)

 

Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.
Executive summary:

The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.

 Daphnid , 48-hr LC50 = 23.265 mg/L (ppm)

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
(Q)SAR
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
results derived from a valid (Q)SAR model and falling into its applicability domain, with adequate and reliable documentation / justification
Justification for type of information:
QSAR prediction: US EPA accepted QSAR method for chemicals properties assessment.
Qualifier:
according to guideline
Guideline:
other: QSAR model
Principles of method if other than guideline:
EPI Suite v 4.0/ECOSARv1.00
GLP compliance:
no
Remarks:
not applicable. QSAR model
Analytical monitoring:
not required
Vehicle:
no
Test organisms (species):
other: Mysid shrimp
Details on test organisms:
Scientific name: Americamysis bahia
Common name: Opossum shrimp, Mysid shrimp, Mysids
Test type:
other: QSAR model
Water media type:
freshwater
Limit test:
no
Total exposure duration:
96 h
Hardness:
not applicable. QSAR model
Test temperature:
not applicable. QSAR model
pH:
not applicable. QSAR model
Dissolved oxygen:
not applicable. QSAR model
Salinity:
not applicable. QSAR model
Nominal and measured concentrations:
not applicable. QSAR model
Details on test conditions:
100% Benzenesulfonic acid, dodecyl-, sodium salt
SMILES : O([Na])S(=O)(=O)c1ccc(cc1)CCCCCCCCCCCC
CHEM : Benzenesulfonic acid, dodecyl-, sodium salt
CAS Num: 25155-30-0
MOL FOR: C18 H29 O3 S1 Na1
MOL WT : 348.48
Log Kow: 3.00 (KowWin estimate)
Wat Sol: 800 mg/L (experimental database)
Reference substance (positive control):
not required
Duration:
96 h
Dose descriptor:
LC50
Effect conc.:
17.429 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.

Overview of QSARs estimations on short-term aquatic toxicity of  

Sodium dodecylbenzenesulfonate/Benzenesulfonic acid, dodecyl-, sodium salt

to Mysid shrimp   

 

Method

 

Results

Remarks

Reference

QSAR

estimate

Mysid Shrimp, 96 -hr, LC50= 17.429 mg/L (ppm)

Mysid Shrimp (SW), 30-day, ChV (chronic value) = 1.121 mg/L (ppm)

 

 

Reliable with restrictions,

QSAR estimated data

ECOSAR Program (v1.00)

     

 Mysid Shrimp, 96 -hr, LC50= 17.429 mg/L (ppm)

Mysid Shrimp (SW), 30-day, ChV (chronic value) = 1.121 mg/L (ppm)

 

 

Validity criteria fulfilled:
yes
Remarks:
US EPA accepted QSAR method for chemicals properties assessment.
Conclusions:
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.
Executive summary:

The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.

 

 

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Dodecylbenzene sulfonic acids (CAS# 27176-87-0 , EC Number; 248-289-4) ) is a very close analogue of Sodium dodecylbenzenesulfonate (CAS No. 25155-30-0, EC Number; 246-680-4) ) and the dissociated acid it readily dissociates in water and release the dodecylbenzene sulfonic anion in solution
Qualifier:
according to guideline
Guideline:
other: DIN 38412 Part 11.
Principles of method if other than guideline:
All parameters were maintained in accordance with acceptable DIN guidelines.
GLP compliance:
not specified
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
NR
Test temperature:
20 °C
pH:
8.4
Dissolved oxygen:
9.8 mg/l
Salinity:
NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 12000 NR TO NR 12000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 12000 NR TO NR 12000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 20 NR TO NR C
pH: 8.4 NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: 325 NR TO NR μS/cm
DISSOLVED OXYGEN: 9.8 NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC0
Effect conc.:
6 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC100
Effect conc.:
14 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Validity criteria fulfilled:
yes
Conclusions:
A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
Executive summary:

A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
Dodecylbenzene sulfonic acids (CAS# 27176-87-0 , EC Number; 248-289-4) ) is a very close analogue of Sodium dodecylbenzenesulfonate (CAS No. 25155-30-0, EC Number; 246-680-4) ) and the dissociated acid it readily dissociates in water and release the dodecylbenzene sulfonic anion in solution
Qualifier:
according to guideline
Guideline:
other: Directive 84/449/EEC, C.2 "Acute toxicity for Daphnia"
Principles of method if other than guideline:
River Po waters were collected at the end of the drainage basin, extracted by means of XAD-2 resins and tested for toxicity on aquatic organisms (Daphnia magna and Selenastrum capricornutum) and for mutagenicity with a modified Ames test. The extracts were analyzed by HRGC/FID and selective detectors for the determination of the most common classes of toxic compounds.
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna (Water Flea)
AGE: NR NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
flow-through
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
NR
Test temperature:
20 °C
Salinity:
NR
Nominal and measured concentrations:
TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 5880 NR TO NR 5880 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 48 hour(s)
STAND DUR. (D): 2 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGES STANDARD CONC (ug/L) IONCIC FRACTION
F 5880 NR TO NR 5880 ug/L NR

Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.88 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Validity criteria fulfilled:
yes
Conclusions:
A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
Executive summary:

A 48 h EC50 of 5.88 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Justification for type of information:
C12 LAS; Linear Alkylbenzene Sulfonate (LAS)) is a very close analogue of Sodium dodecylbenzenesulfonate
Qualifier:
according to guideline
Guideline:
other: EPA-660/3-75-009 1975. Method for acute toxicity tests with fish, macroinvertebrates and amphibians.
Principles of method if other than guideline:
Acute toxicity tests were conducted on high molecular weight LAS, individual pure homologues, non-linear LAS components (dialkyl tetralin or indane sulfonates (DTIS), and model biodegradation intermediates (sulfophenyl undecane, SØU) in order to determine whether biodegradation decreases toxicity. In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three beakers. No food was added for the duration of the test.
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three replicate beakers per concentration. Five concentrations were tested.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna (Water fleas); <18 hr old
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Post exposure observation period:
open system
Hardness:
250 mg/L
Test temperature:
not reported
pH:
not reported
Details on test conditions:
This test were performed in 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness. Ten daphnia (<18 hr old) were placed in each of 3 replicate beakers.No food was added.
Reference substance (positive control):
not required
Duration:
24 h
Dose descriptor:
LC50
Effect conc.:
10.7 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: C12 -LAS
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
3.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: C12 -LAS
Details on results:
A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

 Results:The following table shows the acute toxicity of original materials (LC50, mg/L)..

Daphnia magna

 

Average chain length

24 hour

48 hour

High molecular weight LAS

13.3

2.6 ± 0.1

2.3 ± 0.1

Individual homologues LAS

 

 

 

C10

10

53.1 ± 0.4

12.3 ± 2.6

C11

11

15.8 ± 3.0

5.7 ± 0.6

C12

12

10.7 ± 1.6

3.5 ± 1.0

C13

13

2.7 ± 0.4

2.0 ± 0.3

C14

14

1.2 ± 0.2

0.7 ± 0.2

Nonlinear LAS components (DTIS)

 

 

 

C10

10

106.0 ± 27.0

98.0 ± 21.3

C12

12

55.1 ± 9.1

34.1 ± 5.1

C14

14

12.4 ± 1.4

10.0 ± 1.0

Model biodegradation intermediates

 

 

 

C4 (SØ Butyrate)

4

~12,000

~6,000

C5 (SØ Valerate)

5

~12,000

~5,000

C11 (SØU)

11

355 ± 150*

208 ± 85*

*Subsequent repurification of this sample yielded a product with the same isomeric composition but with LC50values over 1000 mg/L for daphnids (Swisher et al., 1976).

 

Validity criteria fulfilled:
yes
Conclusions:
A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
Executive summary:

The toxicity of high molecular weight LAS, individual LAS homologues, and nonlinear LAS components (DTIS) was measured in a series of aquatic toxicity tests with Daphnia magna. Results show that biodegradation of LAS influences the toxicity, with the remaining LAS becoming less toxic. The 48 -hour LC50 of high molecular weight LAS to Daphnia magna is 2.4 mg/L. A 48 h LC50 of 3.5 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
weight of evidence
Study period:
May 8, 2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna, <24 h old.
No.of daphnids: 5/replicate, 4 replicates/treatment.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
hardness 168 mg/l (CaCO3)
Test temperature:
20±2°C
pH:
pH 8.4-8.5;
Dissolved oxygen:
O2 91-97%;
Nominal and measured concentrations:
Concentration Nominal: 1.56, 3.13, 6.25, 12.5, 25, 50 and 100 mg/L
(no vehicle), untreated control
Details on test conditions:
Test conditions :Static; at 20±2°C in 225 mL crystallising dishes (covered), containing 100 mL of reconstituted water of hardness 168 mg/l (CaCO3) and pH 8.3, 16 h light.
Phys. meas.: At 0 and 48 h in one replicate for all concentrations; overall ranges for pH 8.4-8.5; O2 91-97%; temperature (0, 24 and 48 h) 20-21°C.
Observations: Immobility/mortality at 24 and 48 h.
Reference substance (positive control):
not required
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
2.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: (95% CI 2.2-2.7 mg/L)
Details on results:
The 48-h EC50 calculated using trimmed SPK was 2.5 mg/L (95% CI 2.2-2.7 mg/L) ⇔ 2.2 mg a.i./L (95% CI 2.0-2.4 mg a.i./L).
Validity criteria fulfilled:
yes
Conclusions:
A 48 h EC50 of 2.5mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
Executive summary:

A 48 h EC50 of 2.5 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
weight of evidence
Study period:
May 8, 2000
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
no
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
Species: Daphnia magna, <24 h old.
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Test temperature:
20±1 degree C
pH:
pH 7.8-8.0
Dissolved oxygen:
dissolved oxygen 7.7-8.0;
Nominal and measured concentrations:
0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L
Details on test conditions:
Seven concentrations and a control were tested (0.4, 0.7, 1.2, 2.0, 3.5, 6.0, and 10 mg/L). Physicochemical parameters were maintained within acceptable ranges (temperature 20±1 degree C; dissolved oxygen 7.7-8.0; pH 7.8-8.0). At 48 hours, 0/20 daphnids were immobile in the 0.4 through 3.5 mg/L concentrations, 14/20 in 6.0 mg/L, and 20/20 in 10 mg/L. Graphical interpolation was used to calculate the EC50 value.
Reference substance (positive control):
not required
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
5.2 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Remarks on result:
other: (95% 3.5-6.0 mg/L)
Details on results:
A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
Validity criteria fulfilled:
yes
Conclusions:
A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.
Executive summary:

A 48 h EC50 of 5.2 mg/L has been determined for the effects of this substance on Immobility of the Daphnia magna.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
no guideline followed
Principles of method if other than guideline:
The effects of four concentrations of dodecyl benzene sulfonate at four salinity levels on adult Tisbe bulbisetosa were studied in laboratory experiments. The DBS solutions were analysed by the methylene method to control their stability with time.
The stock solution has been prepared by dissolving 100 mg of pure DBS to 100 mL of distilled water. Test solutions for this test have been obtained both by diluting 3, 6, 9, 12 mL of the stock solution in filtered seawater at a determined level of salinity to 1000 mL, and by diluting the fixed amount stock solution in seawater of 9, 12, 18, 36‰ salinity.
GLP compliance:
not specified
Analytical monitoring:
yes
Vehicle:
no
Details on test solutions:
The stock solution has been prepared by dissolving 100 mg of pure DBS to 100 mL of distilled water. Test solutions for this test have been obtained both by diluting 3, 6, 9, 12 mL of the stock solution in filtered seawater at a determined level of salinity to 1000 mL, and by diluting the fixed amount stock solution in seawater of 9, 12, 18, 36‰ salinity.
Test organisms (species):
other aquatic crustacea: Tisbe bulbisetosa
Details on test organisms:
Tisbe bulbisetosa : adult female
Test type:
not specified
Water media type:
saltwater
Limit test:
no
Total exposure duration:
48 h
Nominal and measured concentrations:
This study is the combined effects of four concentrations (0, 3, 6, 9, 12 ppm) of DBS at four salinity (9, 12, 18, 360) on adult Tisbe bulbisetosa.
Reference substance (positive control):
not required
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
4.58 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (18‰ salinity) = 4.58 ppm(95% confidence interval: 4.00 to 5.24)
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
5.12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Remarks on result:
other: (36‰ salinity) = 5.12 ppm(95% confidence interval: 4.27 to 6.13)-
Details on results:
- 48 hr-LC50 (36‰ salinity) = 5.12 ppm(95% confidence interval: 4.27 to 6.13)- 48 hr-LC50 (18‰ salinity) = 4.58 ppm(95% confidence interval: 4.00 to 5.24)

A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.
Validity criteria fulfilled:
yes
Conclusions:
A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.
Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
other: published data
Adequacy of study:
weight of evidence
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
according to guideline
Guideline:
other: EPA-660/3-75-009
Principles of method if other than guideline:
Environmnetal Protection Agency (1975) ; EPA-660/3-75-009, Method for acute toxicity tests with fish, macroinertebrates and amphibians
GLP compliance:
not specified
Analytical monitoring:
not specified
Vehicle:
no
Details on test solutions:
In 250 mL beakers with 200 mL of well water of approximately 250 mg/L hardness, ten Daphnia, less than 18 hours old, were placed in each of the three replicate beakers per concentration. Five concentrations were tested.
Test organisms (species):
Daphnia magna
Details on test organisms:
Daphnia magna <24 hr old
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
165 mg/L (as CaCO3)
Test temperature:
21-23 deg C
pH:
8.1-8.9
Dissolved oxygen:
8.4 ppm
Nominal and measured concentrations:
- five test concentrations and control (nominal)

Details on test conditions:
- Dilution water was a reconstituted water.(average Hardness (as CaCO3): 165 mg/L, Alkalinity (as CaCO3): 120 +/- 10 mg/L, Dissolved oxygen 5.3mg/L (>60% saturation), temperature : 22 +/- 1 deg C, pH : 7.9-8.4)- Number of replicates; 3 replicates- light intensity (12hr photoperiod at 40-70 L/ft^2)- The 250 mL glass test chambers contained 200ml of test water and five test Daphnia.- five concentrations and control
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
LC50
Effect conc.:
1.8 - 5.6 mg/L
Nominal / measured:
nominal
Conc. based on:
act. ingr.
Basis for effect:
mortality
Remarks on result:
other: C12 -LAS
Details on results:
A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
Validity criteria fulfilled:
yes
Conclusions:
A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
Executive summary:

A 48 h LC50 of 1.8 - 5.6 mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna
(Water Flea)
AGE: <24 h
LIFE STAGE: neonate
COMMENT: FEMALE,CLONE A STRAIN
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
48 h
Hardness:
NR
Test temperature:
21 °C
pH:
NR
Dissolved oxygen:
NR
Salinity:
NR
Nominal and measured concentrations:
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: mg/L miligram per liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 6.3 NR TO NR 6300 ug/L NR
F 7.5 NR TO NR 7500 ug/L NR
F 9.5 NR TO NR 9500 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: C (sufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 48 hour(s)
STAND DUR. (D): 2 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: mg/L miligram per liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 6.3 NR TO NR 6300 ug/L NR
F 7.5 NR TO NR 7500 ug/L NR
F 9.5 NR TO NR 9500 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 21 NR TO NR C
pH: NR NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: NR NR TO NR μS/cm
DISSOLVED OXYGEN: NR NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
6.3 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
7.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Duration:
48 h
Dose descriptor:
EC50
Effect conc.:
9.5 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mortality
Details on results:
A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.

Validity criteria fulfilled:
yes
Conclusions:
A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.
Executive summary:

A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.

Endpoint:
short-term toxicity to aquatic invertebrates
Type of information:
experimental study
Adequacy of study:
key study
Reliability:
2 (reliable with restrictions)
Rationale for reliability incl. deficiencies:
study well documented, meets generally accepted scientific principles, acceptable for assessment
Qualifier:
equivalent or similar to guideline
Guideline:
OECD Guideline 202 (Daphnia sp. Acute Immobilisation Test)
GLP compliance:
yes
Analytical monitoring:
no
Vehicle:
no
Test organisms (species):
Daphnia magna
Details on test organisms:
SPECIES #
(NAME): 5 Daphnia magna
(Water Flea)
AGE: NR
LIFE STAGE: not reported, unknown
COMMENT: NR
Test type:
static
Water media type:
freshwater
Limit test:
no
Total exposure duration:
24 h
Hardness:
NR
Test temperature:
NR
pH:
NR
Dissolved oxygen:
NR
Salinity:
NR
Nominal and measured concentrations:
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 12000 NR TO NR 12000 ug/L NR
F 17000 NR TO NR 17000 ug/L NR
Details on test conditions:
TEST CONDITIONS
STUDY TYPE:
MEDIA: FW (Fresh water)
LOCATION: LAB (Laboratory, indoor)
CONTROL: I (insufficient control)
EXPOSURE TYPE: S ( static)
APPLICATION FREQ.: 1 X
EXPOSURE DUR.: 24 hour(s)
STAND DUR. (D): 1 day(s
TEST CONCENTRATION
CHEM ANAL. METHOD: unmeasured
UNIT OF MEASURE: ug/L micrograms per liter

TYPE VALUE RANGE STANDARD CONC (ug/L) ION
F 12000 NR TO NR 12000 ug/L NR
F 17000 NR TO NR 17000 ug/L NR

WATER QUALITY VALUE RANGE (UNIT)
TEMPERATURE: 21 NR TO NR C
pH: NR NR TO NR
HARDNESS: NR NR TO NR mg/L CaCO3
SALINITY: NR NR TO NR
ALKALINITY: NR NR TO NR mg/L CaCO3
CONDUCTIVITY: NR NR TO NR μS/cm
DISSOLVED OXYGEN: NR NR TO NR mg/l
ORGANIC CARBON (NR): NR NR TO NR NR
Reference substance (positive control):
no
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
12 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Duration:
24 h
Dose descriptor:
EC50
Effect conc.:
17 mg/L
Nominal / measured:
nominal
Conc. based on:
test mat.
Basis for effect:
mobility
Details on results:
A 24 h EC50 of 12mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Validity criteria fulfilled:
yes
Conclusions:
A 24 h EC50 of 12mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
Executive summary:

A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

Description of key information

A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna. 
A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.
A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.
The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.
The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.
A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.

Key value for chemical safety assessment

Fresh water invertebrates

Fresh water invertebrates
Effect concentration:
6.3 mg/L

Marine water invertebrates

Marine water invertebrates
Effect concentration:
4.58 mg/L

Additional information

A 48 h EC50 of 6.3 mg/L has been determined for the effects of the test substance on mortality of the Daphnia magna.

A 24 h EC50 of 12 mg/L has been determined for the effects of the test substance on immobilation of the Daphnia magna.

A 48 h LC50 of 3.5mg/L has been determined for the effects of the substance C12 -LAS on mortality of the Daphnia magna.

The ECOSAR QSAR model predicts that LC50 for fresh water species Daphnid would to be 23.265 mg/l therefore that the substance is slightly toxic to Daphnid.

The ECOSAR QSAR model predicts that LC50 for fresh water species cited would to be 17.429 mg/l therefore that the substance is slightly toxic to Mysid shrimp.

A 48 h LC50 of 4.58 mg/L (18‰ salinity) and 5.1 mg/L (36‰ salinity) has been determined for the effects of the substance Dodecylbenzenesulfonate (DBS) on mortality of the Tisbe bulbisetosa.