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EC number: 246-680-4 | CAS number: 25155-30-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Exposure related observations in humans: other data
Administrative data
- Endpoint:
- exposure-related observations in humans: other data
- Type of information:
- experimental study
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- The percutaneous absorption of some anionic surfactants
- Author:
- Howes D.
- Year:
- 1 975
- Bibliographic source:
- J. Soc. Cosmet. Chem. 26: 47-63
Materials and methods
- Type of study / information:
- In vitro penetration through human epidermis
- Endpoint addressed:
- dermal absorption
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- Female abdominal skin samples obtained at autopsy were frozen and stored at- 70 o. Samples of the skin were allowed to thaw out and were heated at 58 o for 2 min and the epidermis removed in sheets The epidermal samples were mounted in 1 cm diameter penetration cells similar to those described by Ainsworth . Saline containing 0 .012 % Pencillin and 0.01% Streptomycin was placed in contact with both surfaces of the sample and the cells were equilibrated at 37 o for 24 h. The electrical resistance of the cells was measured and only cells with a resistanceg reater than 50 000 Ω were used. The saline from the corneum surface was removed and 0.1 ml of the [14C] surfactant solution was placed on the corneum. 1.0 ml aliquots of the saline in the sampling compartment (8.0 ml) were monitored for 14C at 0.5, 1, 2, 3, 4, 6, 7, 8, 24 and 48 h, each time 1.0ml of fresh saline was added to maintain the volume at 8.0 ml. At the end of the experiment the corneum was washed with excess of distilled water and the epidermal sample monitored for 14C by solubilizing in 'Soluene'.
- GLP compliance:
- not specified
Test material
- Reference substance name:
- Sodium dodecylbenzenesulfonate
- EC Number:
- 246-680-4
- EC Name:
- Sodium dodecylbenzenesulfonate
- Cas Number:
- 25155-30-0
- Molecular formula:
- C18H29NaO3S
- IUPAC Name:
- sodium dodecylbenzenesulfonate
- Details on test material:
- - Name of test material (as cited in study report): LAS (CAS #25155-30-0); activity: >99%
- Analytical purity: determined to be chemically pure by thin layer chromatography
- Radiochemical purity (if radiolabelling): determined to be radioactively pure by isotope dilution analysis
- Specific activity (if radiolabelling): > 99%
Constituent 1
Method
- Ethical approval:
- no
- Details on study design:
- SKIN PREPARATION
- Source of skin: human cadavars
- Ethical approval if human skin:
- Type of skin: abdominal
- Preparative technique: Epidermal samples were heated at 58 degrees C for 2 min. Samples were placed in 1 cm diamter penetration cells, and saline with 0.012% penicillin, 0.01% streptomycin was placed on both surfaces of the cells. The cells were equilibrated at 37 degrees C for 24 hrs.
- Membrane integrity check: Only cells with electrical resistance greater than 50,000 ohms were used.
- Storage conditions: -70 degree C - Exposure assessment:
- measured
- Details on exposure:
- Female abdominal skin samples obtained at autopsy were frozen and stored at- 70 o. Samples of the skin were allowed to thaw out and were heated at 58 o for 2 min and the epidermis removed in sheets The epidermal samples were mounted in 1 cm diameter penetration cells similar to those described by Ainsworth . Saline containing 0 .012 % Pencillin and 0.01% Streptomycin was placed in contact with both surfaces of the sample and the cells were equilibrated at 37 o for 24 h. The electrical resistance of the cells was measured and only cells with a resistanceg reater than 50 000 Ω were used. The saline from the corneum surface was removed and 0.1 ml of the [14C] surfactant solution was placed on the corneum. 1.0 ml aliquots of the saline in the sampling compartment (8.0 ml) were monitored for 14C at 0.5, 1, 2, 3, 4, 6, 7, 8, 24 and 48 h, each time 1.0ml of fresh saline was added to maintain the volume at 8.0 ml. At the end of the experiment the corneum was washed with excess of distilled water and the epidermal sample monitored for 14C by solubilizing in 'Soluene'.
Results and discussion
- Results:
- Radiolabelled test substance was applied (0.1 ml of a 3 mM solution) to samples of human abdominal skin from four female cadavars. Exposure time was 48 hrs. Analysis by liquid scintillation counting was done at 0.5, 1, 2, 3, 4, 6, 7, 8, 24, and 48 hrs. Penetration through human skin was negligible, with < 0.07% absorbed in 48 hrs.
Applicant's summary and conclusion
- Conclusions:
- The in vitro penetration through human skin after a 48 hr exposure was < 0.07%.
- Executive summary:
Radiolabelled test substance was applied (0.1 ml of a 3 mM solution) to samples of human abdominal skin from four female cadavars. Exposure time was 48 hrs. Analysis by liquid scintillation counting was done at 0.5, 1, 2, 3, 4, 6, 7, 8, 24, and 48 hrs. Penetration through human skin was negligible, with < 0.07% absorbed in 48 hrs.
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