Registration Dossier
Registration Dossier
Diss Factsheets
Use of this information is subject to copyright laws and may require the permission of the owner of the information, as described in the ECHA Legal Notice.
EC number: 246-680-4 | CAS number: 25155-30-0
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data

Repeated dose toxicity: dermal
Administrative data
- Endpoint:
- short-term repeated dose toxicity: dermal
- Type of information:
- other: published data
- Adequacy of study:
- weight of evidence
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- study well documented, meets generally accepted scientific principles, acceptable for assessment
Data source
Reference
- Reference Type:
- publication
- Title:
- Effect of long term topical application of some anionic surfactants on the skin, oral mucous membrane, and tongue.
- Author:
- Sadai, M. and Mizuno, N.
- Year:
- 1 972
- Bibliographic source:
- Jpn Journal Dermatol. 82:207-221. (in Japanese); cited in: IPCS (1996); Environmental Health Criteria 169: Linear Alkylbenzene Sulfonates (LAS) and Related Compounds. WHO, Geneva, Switzerland.
Materials and methods
Test guideline
- Qualifier:
- no guideline followed
- Principles of method if other than guideline:
- LAS was applied to the backs of the rats. On the 16th day of the experiment, skin at the application site and the tissues of the tongue and oral mucosa (to examine the effects of licking) of the rats that received 30% were examined histologically
- GLP compliance:
- not specified
- Limit test:
- no
Test material
- Reference substance name:
- C12-LAS
- IUPAC Name:
- C12-LAS
- Details on test material:
- Benzenesulfonic acid, C10-13-alkyl derivs., sodium salts.
LAS, activity: 99.9%
Constituent 1
Test animals
- Species:
- rat
- Strain:
- Wistar
- Sex:
- male
Administration / exposure
- Type of coverage:
- occlusive
- Vehicle:
- other: distilled water
- Details on exposure:
- LAS was applied for 15 days to the backs of male rats, at daily doses of 0.5 g of solutions at 20 and 30% (about 286 and 427 mg/kg bw/day).
- Analytical verification of doses or concentrations:
- not specified
- Duration of treatment / exposure:
- 15 days
- Frequency of treatment:
- daily
Doses / concentrations
- Remarks:
- Doses / Concentrations:
286 and 427 mg/kg bw day
Basis:
nominal per unit body weight
- Control animals:
- yes
- Details on study design:
- LAS was applied for 15 days to the backs of male rats, at daily doses of 0.5 g of solutions at 20 and 30% (about 286 and 427 mg/kg bw/day). On the 16th day of the experiment, the animals were assessed. Body weight gain was suppressed in the 20% group (286 mg/kg bw/day) and the body weight was decreased in the 30% group (427 mg/kg bw/day). An infiltrating, yellowish-reddish brown crust was observed after 2-3 days in the lower dose group, and after 1-2 days in the high dose group. After 4-6 days the crust was abraded and erosion occurred at the abraded site.
Examinations
- Sacrifice and pathology:
- On the 16th day of the experiment, skin at the application site and the tissues of the tongue and oral mucosa (to examine the effects of licking) of the rats that received 30% were examined histologically
Results and discussion
Results of examinations
- Clinical signs:
- no effects observed
- Mortality:
- no mortality observed
- Body weight and weight changes:
- effects observed, treatment-related
- Ophthalmological findings:
- not examined
- Clinical biochemistry findings:
- effects observed, treatment-related
- Urinalysis findings:
- not examined
- Organ weight findings including organ / body weight ratios:
- effects observed, treatment-related
- Histopathological findings: non-neoplastic:
- effects observed, treatment-related
- Details on results:
- Body weight gain was suppressed in the 20% group (286 mg/kg bw/day) and the body weight was decreased in the 30% group (427 mg/kg bw/day). An infiltrating, yellowish-reddish brown crust was observed after 2-3 days in the lower dose group, and after 1-2 days in the high dose group. After 4-6 days the crust was abraded and erosion occurred at the abraded site.
Histological examinations of the application site revealed severe necrosis of the region from the epidermis cuticle to the upper layer of the dermis, severe infiltration of leukocytes in the necrotic site, and diffuse inflammatory cell infiltration of all layers of the corium.
The effects on body weight are to be considered related to the LAS irritation.
Effect levels
open allclose all
- Dose descriptor:
- NOAEL
- Effect level:
- < 286 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- body weight and weight gain
- clinical signs
- mortality
- Dose descriptor:
- LOAEL
- Effect level:
- 286 mg/kg bw/day (nominal)
- Based on:
- test mat.
- Sex:
- male
- Basis for effect level:
- body weight and weight gain
- clinical signs
- mortality
Target system / organ toxicity
- Critical effects observed:
- not specified
Applicant's summary and conclusion
- Conclusions:
- The LOAEL for body weight effects is 286 mg/kg bw/day, the lower dose tested.
The NOAEL for body weight effects is <286 mg/kg bw/day. - Executive summary:
LAS was applied for 15 days to the backs of male rats, at daily doses of 0.5 g of solutions at 20 and 30% (about 286 and 427 mg/kg bw/day). On the 16thday of the experiment, the animals were assessed. Body weight gain was suppressed in the 20% group (286 mg/kg bw/day) and the body weight was decreased in the 30% group (427 mg/kg bw/day). An infiltrating, yellowish-reddish brown crust was observed after 2-3 days in the lower dose group, and after 1-2 days in the high dose group. After 4-6 days the crust was abraded and erosion occurred at the abraded site. Histological examinations of the application site revealed severe necrosis of the region from the epidermis cuticle to the upper layer of the dermis, severe infiltration of leukocytes in the necrotic site, and diffuse inflammatory cell infiltration of all layers of the corium. The effects on body weight are to be considered related to the LAS irritation.
The LOAEL for these effects is 286 mg/kg bw/day, the lower dose tested.
The NOAEL for these effects is<286 mg/kg bw/day.
Information on Registered Substances comes from registration dossiers which have been assigned a registration number. The assignment of a registration number does however not guarantee that the information in the dossier is correct or that the dossier is compliant with Regulation (EC) No 1907/2006 (the REACH Regulation). This information has not been reviewed or verified by the Agency or any other authority. The content is subject to change without prior notice.
Reproduction or further distribution of this information may be subject to copyright protection. Use of the information without obtaining the permission from the owner(s) of the respective information might violate the rights of the owner.
