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EC number: 701-068-0 | CAS number: 2156592-58-2
Long-term toxicity to aquatic invertebrates
Administrative data
- Endpoint:
- long-term toxicity to aquatic invertebrates
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 2 (reliable with restrictions)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study with restictions
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 002
- Report date:
- 2002
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 211 (Daphnia magna Reproduction Test)
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Amines, tallow alkyl
- EC Number:
- 263-125-1
- EC Name:
- Amines, tallow alkyl
- Cas Number:
- 61790-33-8
- IUPAC Name:
- 61790-33-8
- Reference substance name:
- Amines, tallow alkyl
- IUPAC Name:
- Amines, tallow alkyl
- Details on test material:
- - Name of test material (as cited in study report): Tallowalkylamine
Constituent 1
Constituent 2
Test solutions
- Vehicle:
- no
- Details on test solutions:
- PREPARATION AND APPLICATION OF TEST SOLUTION (especially for difficult test substances)
- Dispersion treatment: Stock solution was 10 mg/L. The test item was applied to natural river water. This solution was treated for 30 minutes with ultrasound at 40 °C.
Test organisms
- Test organisms (species):
- Daphnia magna
- Details on test organisms:
- TEST ORGANISM
- Common name: Water flea
- Strain: Daphnia magna STRAUS (Clone 5)
- Source: Institut für Wasser-, Boden- und Lufthygiene (WaBoLu); Breeder DR.U.NOACK-LABORATORIEN, D-31157 Sarstedt, Germany
- Age at study initiation: 2 - 24 h old
- Feeding during test: The daphnids were not fed during the study.
HOUSING:
- Conditions: In 2-3 L glass vessels with approximately 1.8 L culture medium, at 21 °C (temperatures of 20 - 25 °C are tolerated), in an
incubator, 16 h illumination, illumination strength max. 20 μE⋅m-2 ⋅s-1.
- Culture medium: Elendt M4, according to ELENDT (1990), modified to a total hardness of 160 to 180 mg CaCO3/L.
- Type and amount of food: Feeding ad libitum with a mix of Desmodesmus subspicatus and Chlorella vulgaris, with an algae cell density of > 106 cells/mL.
- Feeding frequency: 5x weekly
Study design
- Test type:
- semi-static
- Water media type:
- freshwater
- Limit test:
- no
- Total exposure duration:
- 21 d
Test conditions
- Test temperature:
- 20±2 °C
- Nominal and measured concentrations:
- Nominal concetrations: 0.013, 0.032, 0.08, 0.2, 0.5 mg/L
- Details on test conditions:
- TEST SYSTEM
- Test vessel:
- Material, size, fill volume: Glass beakers (5 cm ID x 8 cm H), 100 mL; Volume of the study medium is 50 mL
- No. of organisms per vessel: 1
- No. of vessels per concentration (replicates): 10
- No. of vessels per control (replicates): 10
- Renewal rate of test solution: 3 times per week ( monday, wednesday and friday)
TEST MEDIUM / WATER PARAMETERS
- Source/preparation of dilution water: A natural river water of agricultural backround, middle reach of the river "Böhme", Lower-Saxony was used.
Test water parameter:
pH: 8.2
Conductivity [μS/cm]: 397
DOC [mg C/L]: 7.3
TOC [mg C/L]: 9.9
Ammonium-N [mg N/L]: 0.141
Nitrate-N [mg N/L]: 2.38
o-Phosphate-P [mg P/L]: 0.031
Total Phosphate [mg P/L]: 0.128
Suspended Matter [mg/L]: 17.4
Total Hardness [mg CO3/L]: 91.3
- Culture medium different from test medium: yes
OTHER TEST CONDITIONS
- Photoperiod: 16/8 hours light dark cycle
- Light intensity: Diffuse light, illumination range max. 20 μE⋅m-2 ⋅ s-1
EFFECT PARAMETERS MEASURED: reproduction and mortality
- Reference substance (positive control):
- yes
- Remarks:
- potassiumdichromate
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 21 d
- Dose descriptor:
- NOEC
- Effect conc.:
- 0.013 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- LOEC
- Effect conc.:
- 0.032 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Duration:
- 21 d
- Dose descriptor:
- EC50
- Effect conc.:
- 0.24 mg/L
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Results with reference substance (positive control):
- - Results with reference substance valid? yes
- Relevant effect levels: EC50 = 2.1 (24h)
Any other information on results incl. tables
Table 1: Summary of all test item related effects in the test concentrations
Effects |
Nominal concentrations (mg/L) |
||||
0.013 |
0.032 |
0.08 |
0.02 |
0.5 |
|
Reproduction rate |
-- |
++ |
++ |
++ |
++ |
Stillborn juveniles |
-- |
-- |
++ |
++ |
-- |
Aborded eggs |
-- |
-- |
-- |
-- |
-- |
immobilization |
-- |
-- |
-- |
-- |
++ |
Dry weight |
-- |
-- |
-- |
-- |
++ |
Lenght |
-- |
-- |
-- |
-- |
++ |
Males/winter eggs |
-- |
-- |
-- |
-- |
-- |
Summary: |
-- |
++ |
++ |
++ |
++ |
-- no significant effect
++ significant effect
Applicant's summary and conclusion
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