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EC number: 701-068-0 | CAS number: 2156592-58-2
- Life Cycle description
- Uses advised against
- Endpoint summary
- Appearance / physical state / colour
- Melting point / freezing point
- Boiling point
- Density
- Particle size distribution (Granulometry)
- Vapour pressure
- Partition coefficient
- Water solubility
- Solubility in organic solvents / fat solubility
- Surface tension
- Flash point
- Auto flammability
- Flammability
- Explosiveness
- Oxidising properties
- Oxidation reduction potential
- Stability in organic solvents and identity of relevant degradation products
- Storage stability and reactivity towards container material
- Stability: thermal, sunlight, metals
- pH
- Dissociation constant
- Viscosity
- Additional physico-chemical information
- Additional physico-chemical properties of nanomaterials
- Nanomaterial agglomeration / aggregation
- Nanomaterial crystalline phase
- Nanomaterial crystallite and grain size
- Nanomaterial aspect ratio / shape
- Nanomaterial specific surface area
- Nanomaterial Zeta potential
- Nanomaterial surface chemistry
- Nanomaterial dustiness
- Nanomaterial porosity
- Nanomaterial pour density
- Nanomaterial photocatalytic activity
- Nanomaterial radical formation potential
- Nanomaterial catalytic activity
- Endpoint summary
- Stability
- Biodegradation
- Bioaccumulation
- Transport and distribution
- Environmental data
- Additional information on environmental fate and behaviour
- Ecotoxicological Summary
- Aquatic toxicity
- Endpoint summary
- Short-term toxicity to fish
- Long-term toxicity to fish
- Short-term toxicity to aquatic invertebrates
- Long-term toxicity to aquatic invertebrates
- Toxicity to aquatic algae and cyanobacteria
- Toxicity to aquatic plants other than algae
- Toxicity to microorganisms
- Endocrine disrupter testing in aquatic vertebrates – in vivo
- Toxicity to other aquatic organisms
- Sediment toxicity
- Terrestrial toxicity
- Biological effects monitoring
- Biotransformation and kinetics
- Additional ecotoxological information
- Toxicological Summary
- Toxicokinetics, metabolism and distribution
- Acute Toxicity
- Irritation / corrosion
- Sensitisation
- Repeated dose toxicity
- Genetic toxicity
- Carcinogenicity
- Toxicity to reproduction
- Specific investigations
- Exposure related observations in humans
- Toxic effects on livestock and pets
- Additional toxicological data
Toxicity to soil macroorganisms except arthropods
Administrative data
- Endpoint:
- toxicity to soil macroorganisms except arthropods: long-term
- Type of information:
- migrated information: read-across based on grouping of substances (category approach)
- Adequacy of study:
- key study
- Reliability:
- 1 (reliable without restriction)
- Rationale for reliability incl. deficiencies:
- other: GLP Guideline study
Cross-reference
- Reason / purpose for cross-reference:
- reference to same study
Data source
Reference
- Reference Type:
- study report
- Title:
- Unnamed
- Year:
- 2 006
- Report date:
- 2006
Materials and methods
Test guideline
- Qualifier:
- according to guideline
- Guideline:
- OECD Guideline 222 (Earthworm Reproduction Test (Eisenia fetida/Eisenia andrei))
- GLP compliance:
- yes (incl. QA statement)
Test material
- Reference substance name:
- Amines, C16-18-alkyl
- EC Number:
- 292-550-5
- EC Name:
- Amines, C16-18-alkyl
- Cas Number:
- 90640-32-7
- Molecular formula:
- R-NH2 R = alkyl mainly C16-18-(even numbered)-alkyl
- IUPAC Name:
- C16-18-(even numbered)-alkylamines
- Test material form:
- solid
- Details on test material:
- - Chemical name: C16-18-(even numbered)-alkylamines
- EC number: 292-550-5
Based on the qualitative and quantitative information on the composition, the sample used is representative of the boundary composition shared and agreed by each registrant.
Constituent 1
Test substrate
- Vehicle:
- yes
- Details on preparation and application of test substrate:
- - Method of mixing into soil: For application quartz sand was used as a vehicle. A mixture comprised of 10 g quartz sand with neccessary quantitiy of the test item was perpared per replicate. The mixture was throughly mixed with the pre-moistured soil with a long handled blender for at least four minutes. Afterwards the soil was moistured to 60 % of its maximum water holding capacity.
Test organisms
- Test organisms (species):
- Eisenia fetida
- Animal group:
- annelids
- Details on test organisms:
- TEST ORGANISM
- Common name: earthworm
- Source: own breeding, original source was the Bundesanstalt für Verbraucherschutz und Landwirtschaft (BVL)
- Age at test initiation (mean and range, SD): 2-12 month (the difference of age dit not deviate by more than one month)
- Weight at test initiation: 0.35 - 0.60 g
Housing:
- Housing conditions: Organisms were bred at the testing facility in covered plasitc vessels (volume 60 L) containing potting compost at 20 ± "°C in the dark
ACCLIMATION
- Acclimation period: 2 days
Study design
- Study type:
- laboratory study
- Substrate type:
- natural soil
- Limit test:
- no
- Total exposure duration:
- 8 wk
Test conditions
- Test temperature:
- 20 ± 2 °C
- pH:
- 5.9 - 6.58
- Moisture:
- 30 ± 3 %
- Details on test conditions:
- TEST SYSTEM
- Test container (material, size): plastic boxes with an inner diameter of 15 cm corresponding to a bottom surface area of 177 cm² and a height of 14 cm.
- Amount of soil or substrate: 600 g natural soil (dry weight)
- No. of organisms per container (treatment): 10
- No. of replicates per treatment group: 4
- No. of replicates per control: 8
- No. of replicates per vehicle control: 4
SOURCE AND PROPERTIES OF SUBSTRATE (if soil)
- Geographic location: LUFA 2.2 soil, Speyer
- Pesticide use history at the collection site: no pesticide treatment in the sampling year and 4 former years.
- Collection procedures: the soil was sampled 13 days prior to use and refrigerated at 4-7 °C in the testing facility until use.
- Sampling depth (cm): ca. 25
- Soil texture (if natural soil)
- % sand: ca 77
- % silt: ca 15
- % clay: 7.9
- Organic carbon (%): 2.33 ± 0.18
- Maximum water holding capacity (in % dry weigth): 49 ± 4.0
OTHER TEST CONDITIONS
- Photoperiod: 16 hours
- Light intensity: 400/800 lux
EFFECT PARAMETERS MEASURED (with observation intervals if applicable) : mortality, weight and reproduction - Nominal and measured concentrations:
- Nominal concatrations: control, 50, 100, 200, 500, 1000 mg/kg dry weight.
- Reference substance (positive control):
- yes
- Remarks:
- Carbendazim
Results and discussion
Effect concentrationsopen allclose all
- Duration:
- 8 wk
- Dose descriptor:
- NOEC
- Effect conc.:
- 200 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks:
- mortality and body weight
- Duration:
- 8 wk
- Dose descriptor:
- LOEC
- Effect conc.:
- 500 mg/kg soil dw
- Nominal / measured:
- nominal
- Conc. based on:
- test mat.
- Basis for effect:
- reproduction
- Remarks:
- mortality and body weight
Applicant's summary and conclusion
- Executive summary:
Body length, eggs per worm and % of gravid worms are shown in Figure 1. Threshold values (NOEC, LOEC) are given in Table 1. Mean values of the single replicates are shown in Appendix B (Table I-IV). Sodium tetrapropenyl benzene sulfonate: The range finding test showed that there was no effect at 135 mg/Kg Sed (dw), whereas we found a total inhibition of all test parameters at 500 mg/Kg Sed (dw). In order to render NOEC and LOEC more precisely, smaller concentration steps were investigated in the main test. The results of the main test agreed well with those of the range finding test. The NOEC was found at 100, the LOEC at 200 mg/Kg Sed (dw). Cocos dimethylamine: The results of the range finding test and the main test are slightly different, however they are in the same order of magnitude (Fig. 1). We took the results of the main test for calculations of NOEC and LOEC, because of the higher number of replicates and thus higher statistical significance. Di hardened tallow dimethyl ammonium chloride: Here we did not find an effect in the range finding test up to the highest tested concentration of 1350 mg/Kg Sed (dw). Thus we investigated higher concentrations (up to 5410 mg/Kg Sed (dw)) in the main test. The NOEC-value was found at 1350, the LOEC-value at 2030 mg/Kg Sed (dw). Tallow alkylamine: For this substance we did not find an effect up to the highest tested concentration of 2030 mg/Kg Sed (dw). Here we reached the limit of solubility. When the stock solution was added to the sediment, a white precipitate occurred at high concentrations.
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